Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Identifying a species-specific region of yeast TF11B in vivo.
Shaw SP
,
Wingfield J
,
Dorsey MJ
,
Ma J
.
???displayArticle.abstract???
The general transcription factor IIB (TFIIB) is required for RNA polymerase II transcription in eukaryotes. It provides a physical link between the TATA-binding protein (TBP) and the RNA polymerase and is a component previously suggested to respond to transcriptional activators in vitro. In this report, we compare the yeast (Saccharomyces cerevisiae) and human forms of the protein in yeast cells to study their functional differences. We demonstrate that human TFIIB fails to functionally replace yeast TFIIB in yeast cells. By analyzing various human-yeast hybrid TFIIB molecules, we show that a 14-amino-acid region at the amino terminus of the first repeat of yeast TFIIB plays an important role in determining species specificity in vivo. In addition, we identify four amino acids in this region that are critical for an amphipathic helix unique to yeast TFIIB. By site-directed mutagenesis analyses we demonstrate that these four amino acids are important for yeast TFIIB's activity in vivo. Finally, we show that mutations in the species-specific region of yeast TFIIB can differentially affect the expression of genes activated by different activators in vivo. These results provide strong evidence suggesting that yeast TFIIB is involved in the process of transcriptional activation in living cells.
Bagby,
Solution structure of the C-terminal core domain of human TFIIB: similarity to cyclin A and interaction with TATA-binding protein.
1995, Pubmed
Bagby,
Solution structure of the C-terminal core domain of human TFIIB: similarity to cyclin A and interaction with TATA-binding protein.
1995,
Pubmed
Baniahmad,
Interaction of human thyroid hormone receptor beta with transcription factor TFIIB may mediate target gene derepression and activation by thyroid hormone.
1993,
Pubmed
Barberis,
Delineation of two functional regions of transcription factor TFIIB.
1993,
Pubmed
Berger,
Genetic isolation of ADA2: a potential transcriptional adaptor required for function of certain acidic activation domains.
1992,
Pubmed
Boeke,
5-Fluoroorotic acid as a selective agent in yeast molecular genetics.
1987,
Pubmed
Buratowski,
Five intermediate complexes in transcription initiation by RNA polymerase II.
1989,
Pubmed
Buratowski,
Functional domains of transcription factor TFIIB.
1993,
Pubmed
,
Xenbase
Chao,
A mammalian SRB protein associated with an RNA polymerase II holoenzyme.
1996,
Pubmed
Chevray,
Protein interaction cloning in yeast: identification of mammalian proteins that react with the leucine zipper of Jun.
1992,
Pubmed
Colgan,
Interaction between a transcriptional activator and transcription factor IIB in vivo.
1993,
Pubmed
Colicelli,
Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase.
1989,
Pubmed
Cormack,
Functional differences between yeast and human TFIID are localized to the highly conserved region.
1991,
Pubmed
Côté,
Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex.
1994,
Pubmed
Gill,
A highly conserved domain of TFIID displays species specificity in vivo.
1991,
Pubmed
Giniger,
Specific DNA binding of GAL4, a positive regulatory protein of yeast.
1985,
Pubmed
Goodrich,
Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB.
1993,
Pubmed
Ha,
Cloning of a human gene encoding the general transcription initiation factor IIB.
1991,
Pubmed
Ha,
Multiple functional domains of human transcription factor IIB: distinct interactions with two general transcription factors and RNA polymerase II.
1993,
Pubmed
Hahn,
Transcription. Clamping the TBP stirrup.
1995,
Pubmed
Himmelfarb,
GAL11P: a yeast mutation that potentiates the effect of weak GAL4-derived activators.
1990,
Pubmed
Hisatake,
Conserved structural motifs between Xenopus and human TFIIB.
1991,
Pubmed
,
Xenbase
Hoey,
Molecular cloning and functional analysis of Drosophila TAF110 reveal properties expected of coactivators.
1993,
Pubmed
Ingles,
Reduced binding of TFIID to transcriptionally compromised mutants of VP16.
1991,
Pubmed
Joliot,
Interaction with RAP74 subunit of TFIIF is required for transcriptional activation by serum response factor.
1995,
Pubmed
Kim,
Crystal structure of a yeast TBP/TATA-box complex.
1993,
Pubmed
Kim,
Co-crystal structure of TBP recognizing the minor groove of a TATA element.
1993,
Pubmed
Kim,
Proline-rich activator CTF1 targets the TFIIB assembly step during transcriptional activation.
1994,
Pubmed
Kim,
A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II.
1994,
Pubmed
Knaus,
Yeast SUB1 is a suppressor of TFIIB mutations and has homology to the human co-activator PC4.
1996,
Pubmed
Koleske,
A novel transcription factor reveals a functional link between the RNA polymerase II CTD and TFIID.
1992,
Pubmed
Koleske,
An RNA polymerase II holoenzyme responsive to activators.
1994,
Pubmed
Lee,
Model for binding of transcription factor TFIIB to the TBP-DNA complex.
1995,
Pubmed
Lessard,
Two monoclonal antibodies to actin: one muscle selective and one generally reactive.
1988,
Pubmed
Li,
RNA polymerase II initiation factor interactions and transcription start site selection.
1994,
Pubmed
Liao,
A kinase-cyclin pair in the RNA polymerase II holoenzyme.
1995,
Pubmed
Lin,
Mechanism of action of an acidic transcriptional activator in vitro.
1991,
Pubmed
Lin,
Binding of general transcription factor TFIIB to an acidic activating region.
1991,
Pubmed
Ma,
Deletion analysis of GAL4 defines two transcriptional activating segments.
1987,
Pubmed
Maldonado,
News on initiation and elongation of transcription by RNA polymerase II.
1995,
Pubmed
Malik,
Sequence of general transcription factor TFIIB and relationships to other initiation factors.
1991,
Pubmed
Nikolov,
Crystal structure of a TFIIB-TBP-TATA-element ternary complex.
1995,
Pubmed
Ossipow,
A mammalian RNA polymerase II holoenzyme containing all components required for promoter-specific transcription initiation.
1995,
Pubmed
Ozer,
Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription.
1994,
Pubmed
Pinto,
The yeast SUA7 gene encodes a homolog of human transcription factor TFIIB and is required for normal start site selection in vivo.
1992,
Pubmed
Piña,
ADA3: a gene, identified by resistance to GAL4-VP16, with properties similar to and different from those of ADA2.
1993,
Pubmed
Roberts,
Interaction between an acidic activator and transcription factor TFIIB is required for transcriptional activation.
1993,
Pubmed
Roberts,
Activator-induced conformational change in general transcription factor TFIIB.
1994,
Pubmed
Sauer,
Control of transcription by Krüppel through interactions with TFIIB and TFIIE beta.
1995,
Pubmed
Stringer,
Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID.
1990,
Pubmed
Swaffield,
Alterations in a yeast protein resembling HIV Tat-binding protein relieve requirement for an acidic activation domain in GAL4.
1992,
Pubmed
Thompson,
A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast.
1993,
Pubmed
Tsuboi,
RNA polymerase II initiation factor alpha from rat liver is almost identical to human TFIIB.
1992,
Pubmed
Wampler,
Functional analysis of Drosophila transcription factor IIB.
1992,
Pubmed
Wilson,
RNA polymerase II holoenzyme contains SWI/SNF regulators involved in chromatin remodeling.
1996,
Pubmed
Xiao,
Binding of basal transcription factor TFIIH to the acidic activation domains of VP16 and p53.
1994,
Pubmed
Yamashita,
Transcription factor TFIIB sites important for interaction with promoter-bound TFIID.
1993,
Pubmed
Yocum,
Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.
1984,
Pubmed
Zawel,
Common themes in assembly and function of eukaryotic transcription complexes.
1995,
Pubmed
