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Biochem J
1996 Aug 01;317 ( Pt 3):643-6. doi: 10.1042/bj3170643.
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Inhibition of protein synthesis in frog (Xenopus laevis) egg extracts by an antibody against phosphatidylinositol 4,5-bisphosphate.
Keating TJ
,
Fukami K
,
Robinson KR
.
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The antibody kt10, which is directed against the phospholipid PtdIns(4,5)P2, inhibits protein synthesis when added to cytosolic extracts prepared from frog eggs. Addition of stable analogues of diacylglycerol and Ins(1,4,5)P3 failed to rescue the inhibition of translation, suggesting that the effect of the antibody was not to block hydrolysis of PtdIns(4,5)P2. Neomycin, which also binds PtdIns(4,5)P2, produced a similar reduction in protein-synthesis levels in the extract system, supporting the idea that it is the interaction of the antibody with PtdIns(4,5)P2 that is producing the effect.
Baudet,
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Baudet,
How to make and use calcium-specific mini- and microelectrodes.
1994,
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Berridge,
Inositol trisphosphate and calcium signalling.
1993,
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Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
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Carney,
Phosphoinositides in mitogenesis: neomycin inhibits thrombin-stimulated phosphoinositide turnover and initiation of cell proliferation.
1985,
Pubmed
Divecha,
Phospholipid signaling.
1995,
Pubmed
Divecha,
Inositides and the nucleus and inositides in the nucleus.
1993,
Pubmed
Fukami,
Antibody to phosphatidylinositol 4,5-bisphosphate inhibits oncogene-induced mitogenesis.
1988,
Pubmed
Fukami,
Requirement of phosphatidylinositol 4,5-bisphosphate for alpha-actinin function.
1992,
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Garcin,
Herpes simplex virus type-1 immediate-early gene expression and shut off of host protein synthesis are inhibited in neomycin-treated human epidermoid carcinoma 2 cells.
1990,
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Han,
Reducing inositol lipid hydrolysis, Ins(1,4,5)P3 receptor availability, or Ca2+ gradients lengthens the duration of the cell cycle in Xenopus laevis blastomeres.
1992,
Pubmed
,
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Harlan,
Pleckstrin homology domains bind to phosphatidylinositol-4,5-bisphosphate.
1994,
Pubmed
Janmey,
Phosphoinositides and calcium as regulators of cellular actin assembly and disassembly.
1994,
Pubmed
Kanki,
The cell cycle dependence of protein synthesis during Xenopus laevis development.
1991,
Pubmed
,
Xenbase
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Murray,
Cell cycle extracts.
1991,
Pubmed
Niggli,
Lipid-cytoskeleton interactions.
1993,
Pubmed
Sullivan,
Calcium mobilization is required for nuclear vesicle fusion in vitro: implications for membrane traffic and IP3 receptor function.
1993,
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,
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Yao,
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,
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