XB-ART-17888
J Bioenerg Biomembr
1996 Aug 01;284:319-26. doi: 10.1007/bf02110108.
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Connexin expression systems: to what extent do they reflect the situation in the animal?
Willecke K
,
Haubrich S
.
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Intercellular communication is mediated by specialized cell-cell contact areas known as gap junctions. Connexins are the constitutive proteins of gap junction intercellular channels. Various cell expression systems are used to express connexins and, in turn, these expression systems can then be tested for their ability to form functional cell-cell channels. In this review, expression of murine endogenous connexins in primary cells and established cell lines is compared with results obtained by expression of exogenous connexins in Xenopus oocytes and cultured mammalian cells. In addition, first reports on characterization of connexin-deficient mice are discussed.
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References [+] :
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991, Pubmed,
Xenbase
Barrio, Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage. 1991, Pubmed , Xenbase
Bennett, Gap junctions: new tools, new answers, new questions. 1991, Pubmed
Beyer, Gap junctions. 1993, Pubmed
Beyer, Molecular cloning and expression of rat connexin40, a gap junction protein expressed in vascular smooth muscle. 1992, Pubmed
Blackshaw, Low resistance junctions between mesoderm cells during development of trunk muscles. 1976, Pubmed , Xenbase
Brissette, Switch in gap junction protein expression is associated with selective changes in junctional permeability during keratinocyte differentiation. 1994, Pubmed
Bruzzone, Connexin40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins. 1993, Pubmed , Xenbase
Bruzzone, Null mutations of connexin32 in patients with X-linked Charcot-Marie-Tooth disease. 1994, Pubmed , Xenbase
Buehler, Single channel behavior of recombinant beta 2 gap junction connexons reconstituted into planar lipid bilayers. 1995, Pubmed
Bukauskas, Biophysical properties of gap junction channels formed by mouse connexin40 in induced pairs of transfected human HeLa cells. 1995, Pubmed
Bukauskas, Heterotypic gap junction channels (connexin26-connexin32) violate the paradigm of unitary conductance. 1995, Pubmed
Crow, Phosphorylation of connexin43 gap junction protein in uninfected and Rous sarcoma virus-transformed mammalian fibroblasts. 1990, Pubmed
Dahl, Attempts to define functional domains of gap junction proteins with synthetic peptides. 1994, Pubmed , Xenbase
Dahl, Expression of the gap junction proteins connexin31 and connexin43 correlates with communication compartments in extraembryonic tissues and in the gastrulating mouse embryo, respectively. 1996, Pubmed
Dahl, Cell/cell channel formation involves disulfide exchange. 1991, Pubmed
Dahl, Expression of functional cell-cell channels from cloned rat liver gap junction complementary DNA. 1987, Pubmed , Xenbase
Darrow, Expression of multiple connexins in cultured neonatal rat ventricular myocytes. 1995, Pubmed
Delorme, Developmental regulation of connexin 40 gene expression in mouse heart correlates with the differentiation of the conduction system. 1995, Pubmed
Dermietzel, Gap junctions between cultured astrocytes: immunocytochemical, molecular, and electrophysiological analysis. 1991, Pubmed
Donaldson, Reconstitution of channels from preparations enriched in lens gap junction protein MP70. 1992, Pubmed
Eghbali, Expression of gap junction channels in communication-incompetent cells after stable transfection with cDNA encoding connexin 32. 1990, Pubmed
Eghbali, Involvement of gap junctions in tumorigenesis: transfection of tumor cells with connexin 32 cDNA retards growth in vivo. 1991, Pubmed
Elfgang, Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa cells. 1995, Pubmed
Giaume, Gap junctions in cultured astrocytes: single-channel currents and characterization of channel-forming protein. 1991, Pubmed
Giaume, Voltage-clamp analysis of a crayfish rectifying synapse. 1987, Pubmed
Gimlich, Differential regulation of the levels of three gap junction mRNAs in Xenopus embryos. 1990, Pubmed , Xenbase
Harris, Ion channels in single bilayers induced by rat connexin32. 1992, Pubmed
Hennemann, Two gap junction genes, connexin 31.1 and 30.3, are closely linked on mouse chromosome 4 and preferentially expressed in skin. 1992, Pubmed , Xenbase
Hennemann, Characterization of gap junction genes expressed in F9 embryonic carcinoma cells: molecular cloning of mouse connexin31 and -45 cDNAs. 1992, Pubmed
Holder, Gap junction function and cancer. 1993, Pubmed
Jongen, Regulation of connexin 43-mediated gap junctional intercellular communication by Ca2+ in mouse epidermal cells is controlled by E-cadherin. 1991, Pubmed
Kadle, Tissue-specific distribution of differentially phosphorylated forms of Cx43. 1991, Pubmed
Koval, Transfected connexin45 alters gap junction permeability in cells expressing endogenous connexin43. 1995, Pubmed
Laing, Characterization of the gap junction protein, connexin45. 1994, Pubmed
Laird, Turnover and phosphorylation dynamics of connexin43 gap junction protein in cultured cardiac myocytes. 1991, Pubmed
Mazet, Voltage dependence of liver gap-junction channels reconstituted into liposomes and incorporated into planar bilayers. 1992, Pubmed
Mesnil, Negative growth control of HeLa cells by connexin genes: connexin species specificity. 1995, Pubmed
Moreno, Gap junction channels: distinct voltage-sensitive and -insensitive conductance states. 1994, Pubmed
Paul, New functions for gap junctions. 1995, Pubmed
Paul, Connexin46, a novel lens gap junction protein, induces voltage-gated currents in nonjunctional plasma membrane of Xenopus oocytes. 1991, Pubmed , Xenbase
Reaume, Cardiac malformation in neonatal mice lacking connexin43. 1995, Pubmed
Reed, Molecular cloning and functional expression of human connexin37, an endothelial cell gap junction protein. 1993, Pubmed
Rose, Gap-junction protein gene suppresses tumorigenicity. 1993, Pubmed
Rubin, Molecular analysis of voltage dependence of heterotypic gap junctions formed by connexins 26 and 32. 1992, Pubmed , Xenbase
Spray, Characterization of gap junctions between cultured leptomeningeal cells. 1991, Pubmed
Steinberg, Connexin43 and connexin45 form gap junctions with different molecular permeabilities in osteoblastic cells. 1994, Pubmed
Stutenkemper, The hepatocyte-specific phenotype of murine liver cells correlates with high expression of connexin32 and connexin26 but very low expression of connexin43. 1992, Pubmed
Suchyna, Identification of a proline residue as a transduction element involved in voltage gating of gap junctions. 1993, Pubmed , Xenbase
Swenson, Formation of gap junctions by expression of connexins in Xenopus oocyte pairs. 1989, Pubmed , Xenbase
Sáez, Pinealocytes in rats: connexin identification and increase in coupling caused by norepinephrine. 1991, Pubmed
Veenstra, Selective dye and ionic permeability of gap junction channels formed by connexin45. 1994, Pubmed
Veenstra, Connexin37 forms high conductance gap junction channels with subconductance state activity and selective dye and ionic permeabilities. 1994, Pubmed
Verselis, Opposite voltage gating polarities of two closely related connexins. 1994, Pubmed , Xenbase
Werner, Formation of hybrid cell-cell channels. 1989, Pubmed , Xenbase
White, Functional analysis of selective interactions among rodent connexins. 1995, Pubmed , Xenbase
White, The connexin family of intercellular channel forming proteins. 1995, Pubmed
White, Mouse Cx50, a functional member of the connexin family of gap junction proteins, is the lens fiber protein MP70. 1992, Pubmed , Xenbase
White, Selective interactions among the multiple connexin proteins expressed in the vertebrate lens: the second extracellular domain is a determinant of compatibility between connexins. 1994, Pubmed , Xenbase
Willecke, Mouse connexin37: cloning and functional expression of a gap junction gene highly expressed in lung. 1991, Pubmed , Xenbase
Young, Functional assembly of gap junction conductance in lipid bilayers: demonstration that the major 27 kd protein forms the junctional channel. 1987, Pubmed
Zhu, Transfection of C6 glioma cells with connexin 43 cDNA: analysis of expression, intercellular coupling, and cell proliferation. 1991, Pubmed