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TATA-box DNA binding activity and subunit composition for RNA polymerase III transcription factor IIIB from Xenopus laevis.
McBryant SJ
,
Meier E
,
Leresche A
,
Sharp SJ
,
Wolf VJ
,
Gottesfeld JM
.
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The RNA polymerase III transcription initiation factor TFIIIB contains the TATA-box-binding protein (TBP) and polymerase III-specific TBP-associated factors (TAFs). Previous studies have shown that DNA oligonucleotides containing the consensus TATA-box sequence inhibit polymerase III transcription, implying that the DNA binding domain of TBP is exposed in TFIIIB. We have investigated the TATA-box DNA binding activity of Xenopus TFIIIB, using transcription inhibition assays and a gel mobility shift assay. Gel shift competition assays with mutant and nonspecific DNAs demonstrate the specificity of the TFIIIB-TATA box DNA complex. The apparent dissociation constant for this protein-DNA interaction is approximately 0.4 nM, similar to the affinity of yeast TBP for the same sequence. TFIIIB transcriptional activity and TATA-box binding activity cofractionate during a series of four ion-exchange chromatographic steps, and reconstituted transcription reactions demonstrate that the TATA-box DNA-protein complex contains TFIIIB TAF activity. Polypeptides with apparent molecular masses of 75 and 92 kDa are associated with TBP in this complex. These polypeptides were renatured after elution from sodium dodecyl sulfate-gels and tested individually and in combination for TFIIIB TAF activity. Recombinant TBP along with protein fractions containing the 75- and 92-kDa polypeptides were sufficient to reconstitute TFIIIB transcriptional activity and DNA binding activity, suggesting that Xenopus TFIIIB is composed of TBP along with these polypeptides.
Bartholomew,
The subunit structure of Saccharomyces cerevisiae transcription factor IIIC probed with a novel photocrosslinking reagent.
1990, Pubmed
Bartholomew,
The subunit structure of Saccharomyces cerevisiae transcription factor IIIC probed with a novel photocrosslinking reagent.
1990,
Pubmed
Bartholomew,
Two components of Saccharomyces cerevisiae transcription factor IIIB (TFIIIB) are stereospecifically located upstream of a tRNA gene and interact with the second-largest subunit of TFIIIC.
1991,
Pubmed
Buratowski,
Five intermediate complexes in transcription initiation by RNA polymerase II.
1989,
Pubmed
Buratowski,
A suppressor of TBP mutations encodes an RNA polymerase III transcription factor with homology to TFIIB.
1992,
Pubmed
Chiang,
Unique TATA-binding protein-containing complexes and cofactors involved in transcription by RNA polymerases II and III.
1993,
Pubmed
Colbert,
A yeast TFIIB-related factor involved in RNA polymerase III transcription.
1992,
Pubmed
Gottesfeld,
Mitotic repression of RNA polymerase III transcription in vitro mediated by phosphorylation of a TFIIIB component.
1994,
Pubmed
,
Xenbase
Hager,
Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.
1980,
Pubmed
Hartl,
Mitotic repression of transcription in vitro.
1993,
Pubmed
,
Xenbase
Hashimoto,
Conserved structural motifs within the N-terminal domain of TFIID tau from Xenopus, mouse and human.
1992,
Pubmed
,
Xenbase
Henry,
A TBP-TAF complex required for transcription of human snRNA genes by RNA polymerase II and III.
1995,
Pubmed
Hernandez,
TBP, a universal eukaryotic transcription factor?
1993,
Pubmed
Hoopes,
Kinetic analysis of yeast TFIID-TATA box complex formation suggests a multi-step pathway.
1992,
Pubmed
Kassavetis,
S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors.
1990,
Pubmed
Kassavetis,
Cloning, expression, and function of TFC5, the gene encoding the B" component of the Saccharomyces cerevisiae RNA polymerase III transcription factor TFIIIB.
1995,
Pubmed
Kassavetis,
The role of the TATA-binding protein in the assembly and function of the multisubunit yeast RNA polymerase III transcription factor, TFIIIB.
1992,
Pubmed
Khoo,
Conserved functional domains of the RNA polymerase III general transcription factor BRF.
1994,
Pubmed
Kovelman,
Purification and characterization of two forms of human transcription factor IIIC.
1992,
Pubmed
L'Etoile,
Human transcription factor IIIC box B binding subunit.
1994,
Pubmed
Lagna,
Cloning and characterization of an evolutionarily divergent DNA-binding subunit of mammalian TFIIIC.
1994,
Pubmed
Lobo,
A TBP complex essential for transcription from TATA-less but not TATA-containing RNA polymerase III promoters is part of the TFIIIB fraction.
1992,
Pubmed
Léveillard,
Repression and redirection of Saccharomyces cerevisiae tRNA synthesis from upstream of the transcriptional start site.
1993,
Pubmed
López-De-León,
PCF4 encodes an RNA polymerase III transcription factor with homology to TFIIB.
1992,
Pubmed
McBryant,
Repression of vertebrate RNA polymerase III transcription by DNA binding proteins located upstream from the transcription start site.
1995,
Pubmed
,
Xenbase
Meyers,
TATA-binding protein and associated factors in polymerase II and polymerase III transcription.
1993,
Pubmed
Radebaugh,
TATA box-binding protein (TBP) is a constituent of the polymerase I-specific transcription initiation factor TIF-IB (SL1) bound to the rRNA promoter and shows differential sensitivity to TBP-directed reagents in polymerase I, II, and III transcription factors.
1994,
Pubmed
Sadowski,
Targeting TBP to a non-TATA box cis-regulatory element: a TBP-containing complex activates transcription from snRNA promoters through the PSE.
1993,
Pubmed
Segall,
Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
1980,
Pubmed
Sinn,
Cloning and characterization of a TFIIIC2 subunit (TFIIIC beta) whose presence correlates with activation of RNA polymerase III-mediated transcription by adenovirus E1A expression and serum factors.
1995,
Pubmed
Starr,
DNA bending is an important component of site-specific recognition by the TATA binding protein.
1995,
Pubmed
Stutz,
Oocyte and somatic tyrosine tRNA genes in Xenopus laevis.
1989,
Pubmed
,
Xenbase
Taggart,
The TATA-binding protein and associated factors are components of pol III transcription factor TFIIIB.
1992,
Pubmed
Tower,
Polymerase III transcription factor B activity is reduced in extracts of growth-restricted cells.
1988,
Pubmed
,
Xenbase
Wang,
Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains.
1995,
Pubmed
Wang,
RNA polymerase II/III transcription specificity determined by TATA box orientation.
1995,
Pubmed
Werner,
Interaction between a complex of RNA polymerase III subunits and the 70-kDa component of transcription factor IIIB.
1993,
Pubmed
White,
Regulation of RNA polymerase III transcription in response to F9 embryonal carcinoma stem cell differentiation.
1989,
Pubmed
White,
A role for the TATA-box-binding protein component of the transcription factor IID complex as a general RNA polymerase III transcription factor.
1992,
Pubmed
White,
Mitotic regulation of a TATA-binding-protein-containing complex.
1995,
Pubmed
Whitehall,
The symmetry of the yeast U6 RNA gene's TATA box and the orientation of the TATA-binding protein in yeast TFIIIB.
1995,
Pubmed
Yoon,
Proximal sequence element-binding transcription factor (PTF) is a multisubunit complex required for transcription of both RNA polymerase II- and RNA polymerase III-dependent small nuclear RNA genes.
1995,
Pubmed