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The effect of PPADS as an antagonist of inositol (1,4,5)trisphosphate induced intracellular calcium mobilization.
Vigne P
,
Pacaud P
,
Urbach V
,
Feolde E
,
Breittmayer JP
,
Frelin C
.
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1. Brain capillary endothelial cells responded to uridine 5'-triphosphate (UTP) and adenosine 5'-triphosphate (ATP) by activation of phospholipase C and by large changes in [Ca2+]i. These cells expressed mRNA sequences identical to the sequence of the P2Y2-purinoceptor of rat pituitaries. 2. Pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS) at 100 microM did not prevent UTP and ATP induced accumulations of total [3H]-inositol (poly)phosphates. It inhibited UTP and ATP induced intracellular Ca2+ mobilization (IC50 = 30 microM) by non competitive mechanism. 3. PPADS (100 microM) inhibited endothelin-1 induced accumulation of total [3H]-inositol (poly)phosphates by less than 20% and prevented most of endothelin-1 induced intracellular Ca2+ mobilization (IC50 = 30 microM). 4. PPADS (100 microM) had no action on ionomycin induced intracellular Ca2+ mobilization. 5. Microinjection of inositol (1,4,5)trisphosphate (InsP3) into Xenopus oocytes induced large Ca2+ activated Cl- currents that were prevented by heparin and by PPADS. 6. It is concluded that PPADS does not recognize rat P2Y2-purinoceptors and prevents UTP and ATP induced intracellular Ca2+ mobilization by a non-specific mechanism that could involve the inhibition of InsP3 channels.
Abbracchio,
Purinoceptors: are there families of P2X and P2Y purinoceptors?
1994, Pubmed
Abbracchio,
Purinoceptors: are there families of P2X and P2Y purinoceptors?
1994,
Pubmed
Brown,
PPADS: an antagonist at endothelial P2Y-purinoceptors but not P2U-purinoceptors.
1995,
Pubmed
Dubyak,
Signal transduction via P2-purinergic receptors for extracellular ATP and other nucleotides.
1993,
Pubmed
Feolde,
ATP, a partial agonist of atypical P2Y purinoceptors in rat brain microvascular endothelial cells.
1995,
Pubmed
Fredholm,
Nomenclature and classification of purinoceptors.
1994,
Pubmed
Frelin,
ADP induces inositol phosphate-independent intracellular Ca2+ mobilization in brain capillary endothelial cells.
1993,
Pubmed
Guillemare,
Effects of the level of mRNA expression on biophysical properties, sensitivity to neurotoxins, and regulation of the brain delayed-rectifier K+ channels Kv1.2.
1992,
Pubmed
,
Xenbase
Guillemette,
Differential effects of heparin on inositol 1,4,5-trisphosphate binding, metabolism, and calcium release activity in the bovine adrenal cortex.
1989,
Pubmed
Ho,
A novel P2-purinoceptor expressed by a subpopulation of astrocytes from the dorsal spinal cord of the rat.
1995,
Pubmed
Lambrecht,
PPADS, a novel functionally selective antagonist of P2 purinoceptor-mediated responses.
1992,
Pubmed
Parker,
Inositol tetrakisphosphate liberates stored Ca2+ in Xenopus oocytes and facilitates responses to inositol trisphosphate.
1991,
Pubmed
,
Xenbase
Vigne,
Endothelin stimulates phosphatidylinositol hydrolysis and DNA synthesis in brain capillary endothelial cells.
1990,
Pubmed
Vigne,
Competitive and non competitive interactions of BQ-123 with endothelin ETA receptors.
1993,
Pubmed
Vigne,
Characterization of the effects of 2-methylthio-ATP and 2-chloro-ATP on brain capillary endothelial cells: similarities to ADP and differences from ATP.
1994,
Pubmed
Vigne,
A new type of amiloride-sensitive cationic channel in endothelial cells of brain microvessels.
1989,
Pubmed
Ziganshin,
PPADS selectively antagonizes P2X-purinoceptor-mediated responses in the rabbit urinary bladder.
1993,
Pubmed
