Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Structure, functional expression, and cerebral localization of the levocabastine-sensitive neurotensin/neuromedin N receptor from mouse brain.
Mazella J
,
Botto JM
,
Guillemare E
,
Coppola T
,
Sarret P
,
Vincent JP
.
???displayArticle.abstract???
This work describes the cloning and expression of the levocabastine-sensitive neurotensin (NT) receptor from mouse brain. The receptor protein comprises 417 amino acids and bears the characteristics of G-protein-coupled receptors. This new NT receptor (NTR) type is 39% homologous to, but pharmacologically distinct from, the only other NTR cloned to date from the rat brain and the human HT29 cell line. When the receptor is expressed in Xenopus laevis oocytes, the H1 antihistaminic drug levocabastine, like NT and neuromedin N, triggers an inward current. The pharmacological properties of this receptor correspond to those of the low-affinity, levocabastine-sensitive NT binding site described initially in membranes prepared from rat and mouse brain. It is expressed maximally in the cerebellum, hippocampus, piriform cortex, and neocortex of adult mouse brain.
Baldwin,
The probable arrangement of the helices in G protein-coupled receptors.
1993, Pubmed
Baldwin,
The probable arrangement of the helices in G protein-coupled receptors.
1993,
Pubmed
Chabry,
Stable expression of the cloned rat brain neurotensin receptor into fibroblasts: binding properties, photoaffinity labeling, transduction mechanisms, and internalization.
1994,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Cullen,
Use of eukaryotic expression technology in the functional analysis of cloned genes.
1987,
Pubmed
Dobner,
Cloning and sequence analysis of cDNA for the canine neurotensin/neuromedin N precursor.
1987,
Pubmed
Dubuc,
The nonpeptide neurotensin antagonist, SR 48692, used as a tool to reveal putative neurotensin receptor subtypes.
1994,
Pubmed
Elde,
Localization of neuropeptide receptor mRNA in rat brain: initial observations using probes for neurotensin and substance P receptors.
1990,
Pubmed
Feurle,
Identification of xenin, a xenopsin-related peptide, in the human gastric mucosa and its effect on exocrine pancreatic secretion.
1992,
Pubmed
,
Xenbase
Gully,
Biochemical and pharmacological profile of a potent and selective nonpeptide antagonist of the neurotensin receptor.
1993,
Pubmed
Hermans,
Phospholipase C activation by neurotensin and neuromedin N in Chinese hamster ovary cells expressing the rat neurotensin receptor.
1992,
Pubmed
Kitabgi,
Two populations of neurotensin binding sites in murine brain: discrimination by the antihistamine levocabastine reveals markedly different radioautographic distribution.
1987,
Pubmed
Kong,
Mutation of an aspartate at residue 89 in somatostatin receptor subtype 2 prevents Na+ regulation of agonist binding but does not alter receptor-G protein association.
1993,
Pubmed
Kozak,
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
1987,
Pubmed
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Labbé-Jullié,
In vivo and in vitro structure-activity studies with peptide and pseudopeptide neurotensin analogs suggest the existence of distinct central neurotensin receptor subtypes.
1994,
Pubmed
Laduron,
Functional consequences of retrograde axonal transport of receptor-bound neurotensin.
1995,
Pubmed
Lomasney,
Expansion of the alpha 2-adrenergic receptor family: cloning and characterization of a human alpha 2-adrenergic receptor subtype, the gene for which is located on chromosome 2.
1990,
Pubmed
Masu,
cDNA cloning of bovine substance-K receptor through oocyte expression system.
,
Pubmed
,
Xenbase
Mazella,
Monoiodo-[Trp11]neurotensin, a highly radioactive ligand of neurotensin receptors. Preparation, biological activity, and binding properties to rat brain synaptic membranes.
1983,
Pubmed
Palacios,
The ontogeny of brain neurotensin receptors studied by autoradiography.
1988,
Pubmed
Pettibone,
Heterogeneity of [3H]neurotensin bindings: studies with dynorphin, L-156,903 and levocabastine.
1988,
Pubmed
Probst,
Sequence alignment of the G-protein coupled receptor superfamily.
1992,
Pubmed
Sadoul,
Preparation of neurotensin selectively iodinated on the tyrosine 3 residue. Biological activity and binding properties on mammalian neurotensin receptors.
1984,
Pubmed
Schotte,
Different postnatal ontogeny of two [3H]neurotensin binding sites in rat brain.
1987,
Pubmed
Schotte,
Evidence for a displaceable non-specific [3H]neurotensin binding site in rat brain.
1986,
Pubmed
Tanaka,
Structure and functional expression of the cloned rat neurotensin receptor.
1990,
Pubmed
,
Xenbase
Vincent,
Neurotensin receptors: binding properties, transduction pathways, and structure.
1995,
Pubmed
Vita,
Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor.
1993,
Pubmed
Watson,
The rat neurotensin receptor expressed in Chinese hamster ovary cells mediates the release of inositol phosphates.
1992,
Pubmed
Zsürger,
Ontogenesis and binding properties of high-affinity neurotensin receptors in human brain.
1992,
Pubmed
al-Rodhan,
Structure-antinociceptive activity of neurotensin and some novel analogues in the periaqueductal gray region of the brainstem.
1991,
Pubmed
