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Mol Cell Biol
1996 Oct 01;1610:5821-9. doi: 10.1128/MCB.16.10.5821.
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DNA length is a critical parameter for eukaryotic transcription in vivo.
Krebs JE
,
Dunaway M
.
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The organization of eukaryotic chromosomes into topological domains has led to the assumption that DNA topology and perhaps supercoiling are involved in eukaryotic nuclear processes. Xenopus oocytes provide a model system for studying the role of DNA topology in transcription. Linear plasmid templates for RNA polymerases (Pols) I and II are not transcribed in Xenopus oocytes, while circular templates are transcriptionally active. Here we show that circularity is not required for transcription of Pol I or Pol II promoters if the linear template is sufficiently long (> 17 to 19 kb). The Xenopus rRNA (Pol I) promoter is active in central positions on a long linear template but is not transcribed when located near an end. Because supercoils generated by transcription could be retained by viscous drag against the long template, these results are consistent with a supercoiling requirement for this promoter. Surprisingly, the herpes simplex virus thymidine kinase (Pol II) promoter is active even 100 bp from the end of the long template, indicating that template length fulfills a critical parameter for transcription that is not consistent with a supercoiling requirement. These results show that DNA length has unrecognized importance for transcription in vivo.
Benyajati,
Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogaster.
1976, Pubmed
Benyajati,
Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogaster.
1976,
Pubmed
Berg,
Diffusion-driven mechanisms of protein translocation on nucleic acids. 1. Models and theory.
1981,
Pubmed
Berg,
Association kinetics with coupled three- and one-dimensional diffusion. Chain-length dependence of the association rate of specific DNA sites.
1982,
Pubmed
Brill,
Transcription-dependent DNA supercoiling in yeast DNA topoisomerase mutants.
1988,
Pubmed
Cook,
Supercoils in human DNA.
1975,
Pubmed
Dunaway,
Local domains of supercoiling activate a eukaryotic promoter in vivo.
1993,
Pubmed
,
Xenbase
Ehbrecht,
Linear diffusion of restriction endonucleases on DNA.
1985,
Pubmed
Forbes,
Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs.
1983,
Pubmed
,
Xenbase
Giaever,
Supercoiling of intracellular DNA can occur in eukaryotic cells.
1988,
Pubmed
Goodrich,
Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA polymerase II.
1994,
Pubmed
Harland,
Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
1983,
Pubmed
,
Xenbase
Hirose,
In vitro transcription of eukaryotic genes is affected differently by the degree of DNA supercoiling.
1988,
Pubmed
Khoury,
Lac repressor-operator interaction: DNA length dependence.
1990,
Pubmed
Labhart,
Enhancer-like properties of the 60/81 bp elements in the ribosomal gene spacer of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Lin,
The general affinity of lac repressor for E. coli DNA: implications for gene regulation in procaryotes and eucaryotes.
1975,
Pubmed
Liu,
Supercoiling of the DNA template during transcription.
1987,
Pubmed
McKnight,
Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: identification of an upstream control region.
1981,
Pubmed
,
Xenbase
Mizushima-Sugano,
Cell-type-specific transcription of an immunoglobulin kappa light chain gene in vitro.
1986,
Pubmed
Murray,
Construction of artificial chromosomes in yeast.
,
Pubmed
Murray,
Chromosome length controls mitotic chromosome segregation in yeast.
1986,
Pubmed
Parvin,
DNA topology and a minimal set of basal factors for transcription by RNA polymerase II.
1993,
Pubmed
Paulson,
The structure of histone-depleted metaphase chromosomes.
1977,
Pubmed
Perucho,
Genetic and physical linkage of exogenous sequences in transformed cells.
1980,
Pubmed
Pruitt,
Effect of topological constraint on transcription of ribosomal DNA in Xenopus oocytes. Comparison of plasmid and endogenous genes.
1984,
Pubmed
,
Xenbase
Riggs,
Lac repressor-operator interaction. I. Equilibrium studies.
1970,
Pubmed
Riggs,
The lac repressor-operator interaction. 3. Kinetic studies.
1970,
Pubmed
Schultz,
Topoisomerases and yeast rRNA transcription: negative supercoiling stimulates initiation and topoisomerase activity is required for elongation.
1992,
Pubmed
Sive,
Multiple sequence elements are required for maximal in vitro transcription of a human histone H2B gene.
1986,
Pubmed
Tsao,
Transcription-driven supercoiling of DNA: direct biochemical evidence from in vitro studies.
1989,
Pubmed
Weber,
Simian virus 40 enhancer increases RNA polymerase density within the linked gene.
,
Pubmed
Weintraub,
Expression of transfected DNA depends on DNA topology.
1986,
Pubmed
Winter,
Diffusion-driven mechanisms of protein translocation on nucleic acids. 2. The Escherichia coli repressor--operator interaction: equilibrium measurements.
1981,
Pubmed
Winter,
Diffusion-driven mechanisms of protein translocation on nucleic acids. 3. The Escherichia coli lac repressor--operator interaction: kinetic measurements and conclusions.
1981,
Pubmed
Wu,
Transcription generates positively and negatively supercoiled domains in the template.
1988,
Pubmed
von Hippel,
Facilitated target location in biological systems.
1989,
Pubmed
