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Nucleic Acids Res
1996 Oct 15;2420:4057-62. doi: 10.1093/nar/24.20.4057.
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Repair of DNA damage in a mitochondrial lysate of Xenopus laevis oocytes.
Ryoji M
,
Katayama H
,
Fusamae H
,
Matsuda A
,
Sakai F
,
Utano H
.
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We examined DNA repair activities of a mitochondrial lysate derived from Xenopus laevis oocytes. Plasmid DNA, exposed to HCl, H2O2 or UV light, was used as the substrate for the in vitro repair reaction. DNA synthesis in the lysate was stimulated 2-8-fold by such lesions, indicating the presence of excision repair activities. This repair DNA synthesis was not affected by aphidicolin, but was sensitive to N-ethylmaleimide. Thus the mitochondrial DNA polymerase, i.e., pol gamma is indeed involved in the reaction. Actual repair of the depurinated DNA was demonstrated by using the polymerase chain reaction (PCR), where the amount of the amplified DNA fragment increased significantly if the depurinated template was incubated in the lysate prior to the PCR. UV-irradiated DNA, on the other hand, restored its ability as a PCR template only if the repair reaction was carried out under the light. Therefore, in this system, UV-induced damage is repaired mainly by photoreactivation. These results show that mitochondria of Xenopus oocytes possess excision repair as well as photolyase activities, and that the in vitro repair system described here should be useful for further molecular characterization of such DNA repair machinery.
Brun,
DNA topoisomerase I from mitochondria of Xenopus laevis oocytes.
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Brun,
DNA topoisomerase I from mitochondria of Xenopus laevis oocytes.
1981,
Pubmed
,
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Callen,
Changes in D-loop frequency and superhelicity among the mitochondrial DNA molecules in relation to organelle biogenesis in oocytes of Xenopus laevis.
1983,
Pubmed
,
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Cheng,
8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G----T and A----C substitutions.
1992,
Pubmed
Chi,
Purification and characterization of MSH1, a yeast mitochondrial protein that binds to DNA mismatches.
1994,
Pubmed
Clayton,
The absence of a pyrimidine dimer repair mechanism in mammalian mitochondria.
1974,
Pubmed
Driggers,
Repair of oxidative damage within the mitochondrial DNA of RINr 38 cells.
1993,
Pubmed
Dunon-Bluteau,
DNA synthesis in a mitochondrial lysate of Xenopus laevis oocytes. H strand replication in vitro.
1987,
Pubmed
,
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Floyd,
Role of oxygen free radicals in carcinogenesis and brain ischemia.
1990,
Pubmed
Goscin,
DNA polymerase delta: one polypeptide, two activities.
1982,
Pubmed
Holt,
Deletions of muscle mitochondrial DNA in patients with mitochondrial myopathies.
1988,
Pubmed
Hübscher,
DNA polymerase epsilon: in search of a function.
1992,
Pubmed
Ikegami,
Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-alpha.
1978,
Pubmed
Kalinowski,
Analysis of DNA damage and repair in murine leukemia L1210 cells using a quantitative polymerase chain reaction assay.
1992,
Pubmed
Krokan,
Involvement of eucaryotic deoxyribonucleic acid polymerases alpha and gamma in the replication of cellular and viral deoxyribonucleic acid.
1979,
Pubmed
Lee,
Structural and functional properties of calf thymus DNA polymerase delta.
1981,
Pubmed
Marinos,
Mitochondrial number, cytochrome oxidase and succinic dehydrogenase activity in Xenopus laevis oocytes.
1981,
Pubmed
,
Xenbase
Mullis,
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
1987,
Pubmed
Myers,
Repair of alkylated purines in the hepatic DNA of mitochondria and nuclei in the rat.
1988,
Pubmed
Pettepher,
Repair of alkali-labile sites within the mitochondrial DNA of RINr 38 cells after exposure to the nitrosourea streptozotocin.
1991,
Pubmed
Prakash,
Repair of pyrimidine dimers in nuclear and mitochondrial DNA of yeast irradiated with low doses of ultraviolet light.
1975,
Pubmed
Richter,
Normal oxidative damage to mitochondrial and nuclear DNA is extensive.
1988,
Pubmed
Ryoji,
Minichromosome assembly accompanying repair-type DNA synthesis in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Satoh,
DNA excision-repair defect of xeroderma pigmentosum prevents removal of a class of oxygen free radical-induced base lesions.
1993,
Pubmed
Sugiyama,
Quantitative analysis of age-associated accumulation of mitochondrial DNA with deletion in human hearts.
1991,
Pubmed
Tomkinson,
Mitochondrial endonuclease activities specific for apurinic/apyrimidinic sites in DNA from mouse cells.
1988,
Pubmed
Tomkinson,
Mammalian mitochondrial endonuclease activities specific for ultraviolet-irradiated DNA.
1990,
Pubmed