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Plant Mol Biol
1996 Dec 01;326:1093-101. doi: 10.1007/bf00041393.
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G2-and early-M-specific expression of the NTCYC1 cyclin gene in Nicotiana tabacum cells.
Qin LX
,
Perennes C
,
Richard L
,
Bouvier-Durand M
,
Tréhin C
,
Inzé D
,
Bergounioux C
.
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We have previously reported the isolation of a cDNA encoding a mitotic cyclin, NTCYC1, from a tobacco cell suspension library. Here we describe the expression patterns of NTCYC1 and of Ntsuc1, a suc 1 plant homologue, in synchronized tobacco cell suspensions. Furthermore, the expression pattern of this cyclin is compared to that of Ntcdc2-1, a Nicotiana tabacum homologue of cdc2. While no NTCYC1 transcript was detected in cells synchronized in the G1 and S phases, NTCYC1 expression was observed in late G2 and early M phases, disappearing in the G1' of a new cell cycle. On the other hand, Ntsuc1 and Ntcdc2-1 exhibited a constitutive expression during the cell cycle. A functional analysis performed by microinjecting NTCYC1 mRNA into immature Xenopus oocytes, indicates that NTCYC1 could participate in the control of the G2/M transition in plant cells. Subsequently NTCYC1 expression was used to assess the status of mesophyll cells in expanded leaves of N. tabacum. Depending on leaf position along the shoot axis, a large population of mesophyll cells appeared with a 4C DNA content, suggesting a G2 arrest. It was found that leaves with such a population also contained high levels of NTCYC1 transcripts. With respect to these results concerning a naturally occurring G2-arrested cell population, the regulation of NTCYC1 expression in planta is discussed.
Church,
Genomic sequencing.
1984,
Pubmed
Colas,
Microinjection of suc1 transcripts delays the cell cycle clock in Patella vulgata embryos.
1993,
Pubmed
Dahl,
The D-type alfalfa cyclin gene cycMs4 complements G1 cyclin-deficient yeast and is induced in the G1 phase of the cell cycle.
1995,
Pubmed
Day,
Cloning of a family of cyclins from Arabidopsis thaliana.
1994,
Pubmed
Draetta,
Mammalian G1 cyclins.
1994,
Pubmed
Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed
Ferreira,
Three discrete classes of Arabidopsis cyclins are expressed during different intervals of the cell cycle.
1994,
Pubmed
Ferreira,
Developmental expression of the arabidopsis cyclin gene cyc1At.
1994,
Pubmed
Ferreira,
Control of cell proliferation during plant development.
1994,
Pubmed
Fobert,
Patterns of cell division revealed by transcriptional regulation of genes during the cell cycle in plants.
1994,
Pubmed
Galbraith,
Rapid flow cytometric analysis of the cell cycle in intact plant tissues.
1983,
Pubmed
Girard,
Cyclin A is required for the onset of DNA replication in mammalian fibroblasts.
1991,
Pubmed
Glab,
Olomoucine, an inhibitor of the cdc2/cdk2 kinases activity, blocks plant cells at the G1 to S and G2 to M cell cycle transitions.
1994,
Pubmed
Hadwiger,
The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex.
1989,
Pubmed
Hata,
Isolation and characterization of cDNA clones for plant cyclins.
1991,
Pubmed
,
Xenbase
Hayles,
The fission yeast cell cycle control gene cdc2: isolation of a sequence suc1 that suppresses cdc2 mutant function.
1986,
Pubmed
Hayles,
suc1 is an essential gene involved in both the cell cycle and growth in fission yeast.
1986,
Pubmed
Hemerly,
Genes regulating the plant cell cycle: isolation of a mitotic-like cyclin from Arabidopsis thaliana.
1992,
Pubmed
,
Xenbase
Hindley,
Sucl+ encodes a predicted 13-kilodalton protein that is essential for cell viability and is directly involved in the division cycle of Schizosaccharomyces pombe.
1987,
Pubmed
Hirt,
Alfalfa cyclins: differential expression during the cell cycle and in plant organs.
1992,
Pubmed
Hunt,
Cyclins and their partners: from a simple idea to complicated reality.
1991,
Pubmed
Hunter,
Cyclins and cancer. II: Cyclin D and CDK inhibitors come of age.
1994,
Pubmed
Hunter,
Braking the cycle.
1993,
Pubmed
King,
Mitosis in transition.
1994,
Pubmed
Knoblich,
Synergistic action of Drosophila cyclins A and B during the G2-M transition.
1993,
Pubmed
Kouchi,
Distinct classes of mitotic cyclins are differentially expressed in the soybean shoot apex during the cell cycle.
1995,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Lehner,
The roles of Drosophila cyclins A and B in mitotic control.
1990,
Pubmed
Lew,
A proliferation of cyclins.
1992,
Pubmed
Minshull,
The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.
1990,
Pubmed
,
Xenbase
Morejohn,
Oryzalin, a dinitroaniline herbicide, binds to plant tubulin and inhibits microtubule polymerization in vitro.
1987,
Pubmed
,
Xenbase
Nasmyth,
Control of the yeast cell cycle by the Cdc28 protein kinase.
1993,
Pubmed
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
Ohtsubo,
Human cyclin E, a nuclear protein essential for the G1-to-S phase transition.
1995,
Pubmed
Pagano,
Cyclin A is required at two points in the human cell cycle.
1992,
Pubmed
Peter,
Joining the complex: cyclin-dependent kinase inhibitory proteins and the cell cycle.
1994,
Pubmed
Pines,
Cyclin-dependent kinases: a new cell cycle motif?
1991,
Pubmed
Pines,
Cell proliferation and control.
1992,
Pubmed
Pines,
Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.
1989,
Pubmed
Qin,
Identification of a cell cycle-related gene, cyclin, in Nicotiana tabacum (L.).
1995,
Pubmed
Reed,
G1-specific cyclins: in search of an S-phase-promoting factor.
1991,
Pubmed
Renaudin,
Cloning of four cyclins from maize indicates that higher plants have three structurally distinct groups of mitotic cyclins.
1994,
Pubmed
,
Xenbase
Richardson,
Human cDNAs encoding homologs of the small p34Cdc28/Cdc2-associated protein of Saccharomyces cerevisiae and Schizosaccharomyces pombe.
1990,
Pubmed
Sauter,
Gibberellin promotes histone H1 kinase activity and the expression of cdc2 and cyclin genes during the induction of rapid growth in deepwater rice internodes.
1995,
Pubmed
Savouré,
Isolation of a full-length mitotic cyclin cDNA clone CycIIIMs from Medicago sativa: chromosomal mapping and expression.
1995,
Pubmed
Setiady,
Tobacco mitotic cyclins: cloning, characterization, gene expression and functional assay.
1995,
Pubmed
Soni,
A family of cyclin D homologs from plants differentially controlled by growth regulators and containing the conserved retinoblastoma protein interaction motif.
1995,
Pubmed
Szarka,
Classification and expression of a family of cyclin gene homologues in Brassica napus.
1995,
Pubmed
Whitfield,
The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transition.
1990,
Pubmed
van den Heuvel,
Distinct roles for cyclin-dependent kinases in cell cycle control.
1993,
Pubmed