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J Neurosci
2005 Jun 29;2526:6145-55. doi: 10.1523/JNEUROSCI.1005-05.2005.
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Rapid, nongenomic responses to ecdysteroids and catecholamines mediated by a novel Drosophila G-protein-coupled receptor.
Srivastava DP
,
Yu EJ
,
Kennedy K
,
Chatwin H
,
Reale V
,
Hamon M
,
Smith T
,
Evans PD
.
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Nongenomic response pathways mediate many of the rapid actions of steroid hormones, but the mechanisms underlying such responses remain controversial. In some cases, cell-surface expression of classical nuclear steroid receptors has been suggested to mediate these effects, but, in a few instances, specific G-protein-coupled receptors (GPCRs) have been reported to be responsible. Here, we describe the activation of a novel, neuronally expressed Drosophila GPCR by the insect ecdysteroids ecdysone (E) and 20-hydroxyecdysone (20E). This is the first report of an identified insect GPCR interacting with steroids. The Drosophila melanogaster dopamine/ecdysteroid receptor (DmDopEcR) shows sequence homology with vertebrate beta-adrenergic receptors and is activated by dopamine (DA) to increase cAMP levels and to activate the phosphoinositide 3-kinase pathway. Conversely, E and 20E show high affinity for the receptor in binding studies and can inhibit the effects of DA, as well as coupling the receptor to a rapid activation of the mitogen-activated protein kinase pathway. The receptor may thus represent the Drosophila homolog of the vertebrate "gamma-adrenergic receptors," which are responsible for the modulation of various activities in brain, blood vessels, and pancreas. Thus, DmDopEcR can function as a cell-surface GPCR that may be responsible for some of the rapid, nongenomic actions of ecdysteroids, during both development and signaling in the mature adult nervous system.
Adams,
The genome sequence of Drosophila melanogaster.
2000, Pubmed
Adams,
The genome sequence of Drosophila melanogaster.
2000,
Pubmed
Airriess,
Selective inhibition of adenylyl cyclase by octopamine via a human cloned alpha 2A-adrenoceptor.
1997,
Pubmed
Arakawa,
Cloning, localization, and permanent expression of a Drosophila octopamine receptor.
1990,
Pubmed
Awad,
Postembryonic development of the midline glia in the CNS of Drosophila: proliferation, programmed cell death, and endocrine regulation.
1997,
Pubmed
Balmanno,
Sustained MAP kinase activation is required for the expression of cyclin D1, p21Cip1 and a subset of AP-1 proteins in CCL39 cells.
1999,
Pubmed
Bandoh,
Molecular cloning and characterization of a novel human G-protein-coupled receptor, EDG7, for lysophosphatidic acid.
1999,
Pubmed
Bier,
Localized activation of RTK/MAPK pathways during Drosophila development.
1998,
Pubmed
Bockaert,
Molecular tinkering of G protein-coupled receptors: an evolutionary success.
1999,
Pubmed
Brennan,
Broad-complex, but not ecdysone receptor, is required for progression of the morphogenetic furrow in the Drosophila eye.
2001,
Pubmed
Champlin,
Ecdysteroid coordinates optic lobe neurogenesis via a nitric oxide signaling pathway.
2000,
Pubmed
Champlin,
Ecdysteroid control of cell proliferation during optic lobe neurogenesis in the moth Manduca sexta.
1998,
Pubmed
Christopoulos,
G protein-coupled receptor allosterism and complexing.
2002,
Pubmed
Dhadialla,
New insecticides with ecdysteroidal and juvenile hormone activity.
1998,
Pubmed
Elmogy,
Presence of membrane ecdysone receptor in the anterior silk gland of the silkworm Bombyx mori.
2004,
Pubmed
Evans,
Agonist-specific coupling of G-protein-coupled receptors to second-messenger systems.
1995,
Pubmed
Filardo,
Estrogen-induced activation of Erk-1 and Erk-2 requires the G protein-coupled receptor homolog, GPR30, and occurs via trans-activation of the epidermal growth factor receptor through release of HB-EGF.
2000,
Pubmed
Gazi,
Functional coupling of the human dopamine D2 receptor with G alpha i1, G alpha i2, G alpha i3 and G alpha o G proteins: evidence for agonist regulation of G protein selectivity.
2003,
Pubmed
Hannan,
Temporal and spatial expression patterns of two G-protein coupled receptors in Drosophila melanogaster.
1996,
Pubmed
Haynie,
Development of the eye-antenna imaginal disc and morphogenesis of the adult head in Drosophila melanogaster.
1986,
Pubmed
Hirst,
Noradrenaline receptors on the rat basilar artery.
1982,
Pubmed
Huang,
Distinct roles for Src tyrosine kinase in beta2-adrenergic receptor signaling to MAPK and in receptor internalization.
2004,
Pubmed
Insel,
Forskolin as a tool for examining adenylyl cyclase expression, regulation, and G protein signaling.
2003,
Pubmed
Kenakin,
Drug efficacy at G protein-coupled receptors.
2002,
Pubmed
Kenakin,
Principles: receptor theory in pharmacology.
2004,
Pubmed
Kenakin,
Agonist-receptor efficacy. II. Agonist trafficking of receptor signals.
1995,
Pubmed
Losel,
Nongenomic steroid action: controversies, questions, and answers.
2003,
Pubmed
Luttrell,
Signaling in time and space: G protein-coupled receptors and mitogen-activated protein kinases.
2003,
Pubmed
Lösel,
Nongenomic actions of steroid hormones.
2003,
Pubmed
Nadal,
Nongenomic actions of estrogens and xenoestrogens by binding at a plasma membrane receptor unrelated to estrogen receptor alpha and estrogen receptor beta.
2000,
Pubmed
Nakagawa,
Inhibition of [(3)H]ponasterone a binding by ecdysone agonists in the intact Sf-9 cell line.
2000,
Pubmed
Nakagawa,
Inhibition of [3H]ponasterone A binding by ecdysone agonists in the intact Kc cell line.
2002,
Pubmed
Näsman,
Dual signalling by different octopamine receptors converges on adenylate cyclase in Sf9 cells.
2002,
Pubmed
Pendleton,
A new role for catecholamines: ontogenesis.
1998,
Pubmed
Reale,
The activation of G-protein gated inwardly rectifying K+ channels by a cloned Drosophila melanogaster neuropeptide F-like receptor.
2004,
Pubmed
,
Xenbase
Reale,
Agonist-specific coupling of a cloned Drosophila melanogaster D1-like dopamine receptor to multiple second messenger pathways by synthetic agonists.
1997,
Pubmed
,
Xenbase
Redfern,
Evidence for the presence of makisterone A in Drosophila larvae and the secretion of 20-deoxymakisterone A by the ring gland.
1984,
Pubmed
Robb,
Agonist-specific coupling of a cloned Drosophila octopamine/tyramine receptor to multiple second messenger systems.
1994,
Pubmed
Ropero,
A nonclassical estrogen membrane receptor triggers rapid differential actions in the endocrine pancreas.
2002,
Pubmed
Sachpatzidis,
Identification of allosteric peptide agonists of CXCR4.
2003,
Pubmed
Scheid,
PKB/AKT: functional insights from genetic models.
2001,
Pubmed
Shi,
The binding site of aminergic G protein-coupled receptors: the transmembrane segments and second extracellular loop.
2002,
Pubmed
Spengler,
Differential signal transduction by five splice variants of the PACAP receptor.
1993,
Pubmed
,
Xenbase
Srivastava,
Rapid, nongenomic responses to ecdysteroids and catecholamines mediated by a novel Drosophila G-protein-coupled receptor.
2005,
Pubmed
,
Xenbase
Swatton,
Increased MAP kinase activity in Alzheimer's and Down syndrome but not in schizophrenia human brain.
2004,
Pubmed
Takeda,
Identification of surrogate ligands for orphan G protein-coupled receptors.
2003,
Pubmed
Thummel,
Steroid signaling in plants and insects--common themes, different pathways.
2002,
Pubmed
Truman,
Steroid receptors and nervous system metamorphosis in insects.
1996,
Pubmed
Yawo,
Involvement of cGMP-dependent protein kinase in adrenergic potentiation of transmitter release from the calyx-type presynaptic terminal.
1999,
Pubmed
Zhu,
Cloning, expression, and characterization of a membrane progestin receptor and evidence it is an intermediary in meiotic maturation of fish oocytes.
2003,
Pubmed