Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1997 Mar 01;173:1543-51. doi: 10.1128/MCB.17.3.1543.
Show Gene links
Show Anatomy links
Genes encoding farnesyl cysteine carboxyl methyltransferase in Schizosaccharomyces pombe and Xenopus laevis.
Imai Y
,
Davey J
,
Kawagishi-Kobayashi M
,
Yamamoto M
.
???displayArticle.abstract???
The mam4 mutation of Schizosaccharomyces pombe causes mating deficiency in h- cells but not in h+ cells. h- cells defective in mam4 do not secrete active mating pheromone M-factor. We cloned mam4 by complementation. The mam4 gene encodes a protein of 236 amino acids, with several potential membrane-spanning domains, which is 44% identical with farnesyl cysteine carboxyl methyltransferase encoded by STE14 and required for the modification of a-factor in Saccharomyces cerevisiae. Analysis of membrane fractions revealed that mam4 is responsible for the methyltransferase activity in S. pombe. Cells defective in mam4 produced farnesylated but unmethylated cysteine and small peptides but no intact M-factor. These observations strongly suggest that the mam4 gene product is farnesyl cysteine carboxyl methyltransferase that modifies M-factor. Furthermore, transcomplementation of S. pombe mam4 allowed us to isolate an apparent homolog of mam4 from Xenopus laevis (Xmam4). In addition to its sequence similarity to S. pombe mam4, the product of Xmam4 was shown to have a farnesyl cysteine carboxyl methyltransferase activity in S. pombe cells. The isolation of a vertebrate gene encoding farnesyl cysteine carboxyl methyltransferase opens the way to in-depth studies of the role of methylation in a large body of proteins, including Ras superfamily proteins.
Akopyan,
Proteolytic processing of farnesylated peptides: assay and partial purification from pig brain membranes of an endopeptidase which has the characteristics of E.C. 3.4.24.15.
1994, Pubmed
Akopyan,
Proteolytic processing of farnesylated peptides: assay and partial purification from pig brain membranes of an endopeptidase which has the characteristics of E.C. 3.4.24.15.
1994,
Pubmed
Anderegg,
Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component.
1988,
Pubmed
Armstrong,
cDNA cloning and expression of the alpha and beta subunits of rat Rab geranylgeranyl transferase.
1993,
Pubmed
Ashby,
Isolation and DNA sequence of the STE14 gene encoding farnesyl cysteine: carboxyl methyltransferase.
1993,
Pubmed
Beach,
ran1+ controls the transition from mitotic division to meiosis in fission yeast.
1985,
Pubmed
Beach,
Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation.
1982,
Pubmed
Chen,
cDNA cloning and expression of the peptide-binding beta subunit of rat p21ras farnesyltransferase, the counterpart of yeast DPR1/RAM1.
1991,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Clarke,
Protein isoprenylation and methylation at carboxyl-terminal cysteine residues.
1992,
Pubmed
Clarke,
Protein carboxyl methyltransferases: two distinct classes of enzymes.
1985,
Pubmed
Danjoh,
Enzymic characterization of fission yeast farnesyl transferase: recognition of the -CAAL motif at the C-terminus.
1996,
Pubmed
Davey,
Mating pheromones of the fission yeast Schizosaccharomyces pombe: purification and structural characterization of M-factor and isolation and analysis of two genes encoding the pheromone.
1992,
Pubmed
Edwards,
Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.
1991,
Pubmed
Egel,
Genes involved in mating type expression of fission yeast.
1973,
Pubmed
Egel,
Premeiotic DNA synthesis in fission yeast.
1974,
Pubmed
Egel,
Assessment of pheromone production and response in fission yeast by a halo test of induced sporulation.
1994,
Pubmed
Fukui,
Role of a ras homolog in the life cycle of Schizosaccharomyces pombe.
1986,
Pubmed
Fukui,
Mating pheromone-like diffusible factor released by Schizosaccharomyces pombe.
1986,
Pubmed
Gibbs,
The ras oncogene--an important regulatory element in lower eucaryotic organisms.
1989,
Pubmed
Goodman,
Mutants of Saccharomyces cerevisiae defective in the farnesylation of Ras proteins.
1990,
Pubmed
Grimm,
Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker.
1988,
Pubmed
He,
RAM2, an essential gene of yeast, and RAM1 encode the two polypeptide components of the farnesyltransferase that prenylates a-factor and Ras proteins.
1991,
Pubmed
Hrycyna,
The Saccharomyces cerevisiae STE14 gene encodes a methyltransferase that mediates C-terminal methylation of a-factor and RAS proteins.
1991,
Pubmed
Hrycyna,
Maturation of isoprenylated proteins in Saccharomyces cerevisiae. Multiple activities catalyze the cleavage of the three carboxyl-terminal amino acids from farnesylated substrates in vitro.
1992,
Pubmed
Hrycyna,
Farnesyl cysteine C-terminal methyltransferase activity is dependent upon the STE14 gene product in Saccharomyces cerevisiae.
1990,
Pubmed
Imai,
The fission yeast mating pheromone P-factor: its molecular structure, gene structure, and ability to induce gene expression and G1 arrest in the mating partner.
1994,
Pubmed
Imai,
Identification of a GTPase-activating protein homolog in Schizosaccharomyces pombe.
1991,
Pubmed
Kitamura,
The Schizosaccharomyces pombe mam2 gene encodes a putative pheromone receptor which has a significant homology with the Saccharomyces cerevisiae Ste2 protein.
1991,
Pubmed
Kjaerulff,
Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3.
1994,
Pubmed
Kohl,
Structural homology among mammalian and Saccharomyces cerevisiae isoprenyl-protein transferases.
1991,
Pubmed
Kozak,
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
1987,
Pubmed
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Leiser,
Evidence that Rap1 carboxylmethylation is involved in regulated insulin secretion.
1995,
Pubmed
Li,
Feedback control of mitosis in budding yeast.
1991,
Pubmed
Ma,
Substrate specificity of the isoprenylated protein endoprotease.
1992,
Pubmed
Marcus,
Significance of C-terminal cysteine modifications to the biological activity of the Saccharomyces cerevisiae a-factor mating pheromone.
1991,
Pubmed
Marr,
Saccharomyces cerevisiae STE14 gene is required for COOH-terminal methylation of a-factor mating pheromone.
1990,
Pubmed
Maundrell,
Thiamine-repressible expression vectors pREP and pRIP for fission yeast.
1993,
Pubmed
Mayer,
CDC43 and RAM2 encode the polypeptide subunits of a yeast type I protein geranylgeranyltransferase.
1992,
Pubmed
McLeod,
The product of the mei3+ gene, expressed under control of the mating-type locus, induces meiosis and sporulation in fission yeast.
1987,
Pubmed
Nadin-Davis,
Involvement of ras in sexual differentiation but not in growth control in fission yeast.
1986,
Pubmed
Nielsen,
Pheromone communication in the fission yeast Schizosaccharomyces pombe.
1995,
Pubmed
Obara,
Isolation and characterization of a gene encoding a G-protein alpha subunit from Schizosaccharomyces pombe: involvement in mating and sporulation pathways.
1991,
Pubmed
Ohya,
Yeast CAL1 is a structural and functional homologue to the DPR1 (RAM) gene involved in ras processing.
1991,
Pubmed
Okazaki,
High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe.
1990,
Pubmed
Philips,
Carboxyl methylation of Ras-related proteins during signal transduction in neutrophils.
1993,
Pubmed
Pérez-Sala,
Methylation and demethylation reactions of guanine nucleotide-binding proteins of retinal rod outer segments.
1991,
Pubmed
Rossi,
Dependence of Ypt1 and Sec4 membrane attachment on Bet2.
1991,
Pubmed
Rothstein,
One-step gene disruption in yeast.
1983,
Pubmed
Sapperstein,
Nucleotide sequence of the yeast STE14 gene, which encodes farnesylcysteine carboxyl methyltransferase, and demonstration of its essential role in a-factor export.
1994,
Pubmed
Schafer,
Enzymatic coupling of cholesterol intermediates to a mating pheromone precursor and to the ras protein.
1990,
Pubmed
Schafer,
Protein prenylation: genes, enzymes, targets, and functions.
1992,
Pubmed
Sinensky,
The prenylation of proteins.
1992,
Pubmed
Sipiczki,
Protoplast fusion of Schizosaccharomyces pombe Auxotrophic mutants of identical mating-type.
1977,
Pubmed
Southern,
Gel electrophoresis of restriction fragments.
1979,
Pubmed
Springer,
Protein methylation in behavioural control mechanisms and in signal transduction.
1979,
Pubmed
Stephenson,
Identification of a C-terminal protein carboxyl methyltransferase in rat liver membranes utilizing a synthetic farnesyl cysteine-containing peptide substrate.
1990,
Pubmed
Tanaka,
Schizosaccharomyces pombe map3+ encodes the putative M-factor receptor.
1993,
Pubmed
Volker,
A single activity carboxyl methylates both farnesyl and geranylgeranyl cysteine residues.
1991,
Pubmed
Willer,
Two-step activation of meiosis by the mat1 locus in Schizosaccharomyces pombe.
1995,
Pubmed
Xue,
Solution phase synthesis of Saccharomyces cerevisiae a-mating factor and its analogs.
1990,
Pubmed
Yabana,
Schizosaccharomyces pombe map1+ encodes a MADS-box-family protein required for cell-type-specific gene expression.
1996,
Pubmed
Yamamoto,
The molecular control mechanisms of meiosis in fission yeast.
1996,
Pubmed
Zhang,
cDNA cloning and expression of rat and human protein geranylgeranyltransferase type-I.
1994,
Pubmed