Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cloning and characterization of the urea transporter UT3: localization in rat kidney and testis.
Tsukaguchi H
,
Shayakul C
,
Berger UV
,
Tokui T
,
Brown D
,
Hediger MA
.
???displayArticle.abstract???
Urea transport in the kidney plays an important role in urinary concentration and nitrogen balance. Recently, three types of urea transporters have been cloned, UT1 and UT2 from rat and rabbit kidney and HUT11 from human bone marrow. To elucidate the physiological role of the latter urea transporter, we have isolated the rat homologue (UT3) of HUT11 and studied its distribution of expression and functional characteristics. UT3 cDNA encodes a 384 amino acid residue protein, which has 80% identity to the human HUT11 and 62% identity to rat UT2. Functional expression in Xenopus oocytes induced a large (approximately 50-fold) increase in the uptake of urea compared with water-injected oocytes. The uptake was inhibited by phloretin (0.75 mM) and pCMBS (0.5 mM) (55 and 32% inhibition, respectively). Northern analysis gave a single band of 3.8 kb in kidney inner and outer medulla, testis, brain, bone marrow, spleen, thymus, and lung. In situ hybridization of rat kidney revealed that UT3 mRNA is expressed in the inner stripe of the outer medulla, inner medulla, the papillary surface epithelium, and the transitional urinary epithelium of urinary tracts. Co-staining experiments using antibody against von Willebrand factor showed that UT3 mRNA in the inner stripe of the outer medulla is expressed in descending vasa recta. These data suggest that UT3 in kidney is involved in counter current exchange between ascending and descending vasa recta, to enhance the cortico-papillary osmolality gradient. In situ hybridization of testis revealed that UT3 is located in Sertoli cells of seminiferous tubules. The signal was only detected in Sertoli cells associated with the early stages of spermatocyte development, suggesting that urea may play a role in spermatogenesis.
Agre,
Aquaporin water channels: unanswered questions and unresolved controversies.
1995, Pubmed
Agre,
Aquaporin water channels: unanswered questions and unresolved controversies.
1995,
Pubmed
Arnt-Ramos,
Immunohistochemical localization of argininosuccinate synthetase in the rat brain in relation to nitric oxide synthase-containing neurons.
1992,
Pubmed
Bonventre,
Effect of urea concentration of pelvic fluid on renal concentrating ability.
1980,
Pubmed
Buniatian,
Urea synthesis in brain.
1966,
Pubmed
Chou,
Concentration dependence of urea and thiourea transport in rat inner medullary collecting duct.
1990,
Pubmed
Effros,
Reversible inhibition of urea exchange in rat hepatocytes.
1993,
Pubmed
Fushimi,
Cloning and expression of apical membrane water channel of rat kidney collecting tubule.
1993,
Pubmed
,
Xenbase
Hasegawa,
Functional expression of cAMP-dependent and independent urea transporters in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Hediger,
Structure, regulation and physiological roles of urea transporters.
1996,
Pubmed
,
Xenbase
Howards,
The blood-testis barrier: a morphologic or physiologic phenomenon?
1978,
Pubmed
Knepper,
Pathways of urea transport in the mammalian kidney.
1987,
Pubmed
Knepper,
The vasopressin-regulated urea transporter in renal inner medullary collecting duct.
1990,
Pubmed
Kozak,
Structural features in eukaryotic mRNAs that modulate the initiation of translation.
1991,
Pubmed
Krawetz,
Sequence similarities of the protamine genes: implications for regulation and evolution.
1988,
Pubmed
Macey,
Osmotic stability of red cells in renal circulation requires rapid urea transport.
1988,
Pubmed
Nielsen,
Cellular and subcellular localization of the vasopressin- regulated urea transporter in rat kidney.
1996,
Pubmed
Olives,
Cloning and functional expression of a urea transporter from human bone marrow cells.
1994,
Pubmed
,
Xenbase
Olivès,
Kidd blood group and urea transport function of human erythrocytes are carried by the same protein.
1995,
Pubmed
Pallone,
Transport of sodium and urea in outer medullary descending vasa recta.
1994,
Pubmed
Pallone,
Characterization of the urea transporter in outer medullary descending vasa recta.
1994,
Pubmed
Pallone,
Diffusive transport of solute in the rat medullary microcirculation.
1995,
Pubmed
Piperno,
Microtubules containing acetylated alpha-tubulin in mammalian cells in culture.
1987,
Pubmed
Promeneur,
Evidence for distinct vascular and tubular urea transporters in the rat kidney.
1996,
Pubmed
Sabolić,
Localization of the CHIP28 water channel in rat kidney.
1992,
Pubmed
Sands,
Countercurrent system.
1990,
Pubmed
Sands,
Urea permeability of mammalian inner medullary collecting duct system and papillary surface epithelium.
1987,
Pubmed
Schaeren-Wiemers,
A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes.
1993,
Pubmed
Shayakul,
Molecular cloning and characterization of the vasopressin-regulated urea transporter of rat kidney collecting ducts.
1996,
Pubmed
,
Xenbase
Tabor,
Polyamines.
1984,
Pubmed
Walser,
Urea flux in the ureter.
1988,
Pubmed
You,
Cloning and characterization of the vasopressin-regulated urea transporter.
1993,
Pubmed
,
Xenbase