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J Membr Biol
1997 May 01;1571:53-61. doi: 10.1007/s002329900215.
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Cross talk of bumetanide-sensitive and HCO3--dependent transporters activated by IBMX in renal epithelial A6 cells.
Niisato N
,
Marunaka Y
.
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We studied cAMP-dependent regulation of ion transport in aldosterone-untreated renal epithelial A6 cells by measuring short-circuit current (Isc). Biphasic increases in Isc, a transient phase followed by a sustained one, were elicited in response to 1 mm 3-isobutyl-1-methylxanthine (IBMX, an inhibitor of phosphodiesterase) which increased cytosolic cAMP concentration. IBMX increased the apical Cl- conductance. The sustained phase of Isc induced by IBMX was reduced by 50 microM bumetanide (Na+/K+/2 Cl- cotransporter inhibitor) or 100 microM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS, an inhibitor of Cl-/HCO3- exchanger). Under the normal condition, the inhibitory effect of bumetanide was much larger than that of DIDS. On the other hand, under a low Cl- condition, the effect of DIDS was more effective than that of bumetanide. Further, under a Cl--free condition Na+/HCO3- symporter contributed to the IBMX-generated Isc. Taken together, our observations suggest that in A6 cells (i) IBMX stimulates Cl- secretion associated with an increase in apical Cl- conductances, (ii) the ionic components to generate the IBMX-induced Isc are mainly maintained by bumetanide-sensitive Na+/K+/2 Cl- cotransporter and DIDS-sensitive Cl-/HCO3- exchanger, (iii) Cl-/HCO3- exchanger coupled to Na+/HCO3- symporter under a low-Cl- condition or Na+/HCO3- symporter under a Cl--free condition contributes to the IBMX-induced Isc, compensating for diminishment of the Na+/K+/2Cl- cotransporter-mediated Cl- secretion, (iv) IBMX increases Cl- and HCO3- conductances in the apical membrane.
Baggio,
Functional correlation between the Ser/Thr-phosphorylation of band-3 and band-3-mediated transmembrane anion transport in human erythrocytes.
1993, Pubmed
Baggio,
Functional correlation between the Ser/Thr-phosphorylation of band-3 and band-3-mediated transmembrane anion transport in human erythrocytes.
1993,
Pubmed
Chalfant,
Distinct regulation of Na+ reabsorption and Cl- secretion by arginine vasopressin in the amphibian cell line A6.
1993,
Pubmed
,
Xenbase
Chen,
Role of cyclic AMP-induced Cl conductance in aqueous humour formation by the dog ciliary epithelium.
1994,
Pubmed
Clari,
DIDS-effect on Ser/Thr- and Tyr-phosphorylation of membrane proteins in human erythrocytes.
1992,
Pubmed
De Smet,
Regulatory volume decrease in a renal distal tubular cell line (A6). I. Role of K+ and Cl-.
1995,
Pubmed
,
Xenbase
Dharmsathaphorn,
Mechanism of chloride secretion induced by carbachol in a colonic epithelial cell line.
1986,
Pubmed
Doi,
Antidiuretic hormone decreases the intracellular pH in distal nephron epithelium (A6) by inhibiting Na+/H+ exchange.
1995,
Pubmed
Doi,
Amiloride-sensitive and HCO3(-)-dependent ion transport activated by aldosterone and vasotocin in A6 cells.
1995,
Pubmed
Frizzell,
Sodium-coupled chloride transport by epithelial tissues.
1979,
Pubmed
Fuller,
Forskolin- but not ionomycin-evoked Cl- secretion in colonic epithelia depends on intact microtubules.
1994,
Pubmed
Granitzer,
Effect of dexamethasone on sodium channel block and densities in A6 cells.
1995,
Pubmed
,
Xenbase
Greger,
Cl- -channels in the apical cell membrane of the rectal gland "induced" by cAMP.
1985,
Pubmed
Greger,
Mechanism of NaCl secretion in rectal gland tubules of spiny dogfish (Squalus acanthias). III. Effects of stimulation of secretion by cyclic AMP.
1984,
Pubmed
Guerra,
Vasopressin-dependent control of basolateral Na/H-exchange in highly differentiated A6-cell monolayers.
1993,
Pubmed
,
Xenbase
Haas,
The Na-K-Cl cotransporters.
1994,
Pubmed
He,
Beta-adrenergic control of cell volume and chloride transport in an established rat submandibular cell line.
1989,
Pubmed
Johnsen,
Enhanced sensitivity to stimulation of sodium transport and cyclic AMP by antidiuretic hormone after Ca2+ depletion of isolated frog skin epithelium.
1982,
Pubmed
Kizer,
Vasopressin and cAMP stimulate electrogenic chloride secretion in an IMCD cell line.
1995,
Pubmed
Kleyman,
Amiloride and its analogs as tools in the study of ion transport.
1988,
Pubmed
Lang,
Adenosine stimulates sodium transport in kidney A6 epithelia in culture.
1985,
Pubmed
,
Xenbase
Lang,
Neurohypophysial peptide potencies in cultured anuran epithelia (A6).
1986,
Pubmed
,
Xenbase
Lang,
Complete response to vasopressin requires epithelial organization in A6 cells in culture.
1986,
Pubmed
MacLeod,
Isotonic volume reduction associated with cAMP stimulation of 36Cl efflux from jejunal crypt epithelial cells.
1994,
Pubmed
Mandel,
Characterization of a cyclic AMP-activated Cl-transport pathway in the apical membrane of a human colonic epithelial cell line.
1986,
Pubmed
Marunaka,
Antidiuretic hormone-responding nonselective cation channel in distal nephron epithelium (A6).
1994,
Pubmed
Marunaka,
Effects of insulin and phosphatase on a Ca2(+)-dependent Cl- channel in a distal nephron cell line (A6).
1990,
Pubmed
Marunaka,
Modification of Ca(2+)-sensitivity of Ca(2+)-activated Cl- channel by vasopressin and cholera toxin.
1993,
Pubmed
Marunaka,
Effects of vasopressin on single Cl- channels in the apical membrane of distal nephron cells (A6).
1993,
Pubmed
Marunaka,
Insulin activates single amiloride-blockable Na channels in a distal nephron cell line (A6).
1992,
Pubmed
Marunaka,
Chloride channels in the apical membrane of a distal nephron A6 cell line.
1990,
Pubmed
Marunaka,
Effects of vasopressin and cAMP on single amiloride-blockable Na channels.
1991,
Pubmed
Nagy,
Vasopressin activates a chloride conductance in cultured cortical collecting duct cells.
1994,
Pubmed
Nakahari,
ADH-evoked [Cl-]i-dependent transient in whole cell current of distal nephron cell line A6.
1995,
Pubmed
Nakahari,
Regulation of cell volume by beta2-adrenergic stimulation in rat fetal distal lung epithelial cells.
1996,
Pubmed
Niisato,
Hyposmolality-induced enhancement of ADH action on amiloride-sensitive Isc in renal epithelial A6 cells.
1997,
Pubmed
Perkins,
Transport properties of toad kidney epithelia in culture.
1981,
Pubmed
,
Xenbase
Raat,
Regulation of Na(+)-K(+)-2Cl- cotransport activity in rabbit proximal tubule primary culture.
1994,
Pubmed
Shintani,
Regulation of chloride channel trafficking by cyclic AMP via protein kinase A-independent pathway in A6 renal epithelial cells.
1996,
Pubmed
Van Scott,
Intracellular Ca2+ and regulation of ion transport across rabbit Clara cells.
1992,
Pubmed
Verrey,
Antidiuretic hormone action in A6 cells: effect on apical Cl and Na conductances and synergism with aldosterone for NaCl reabsorption.
1994,
Pubmed
,
Xenbase
Wills,
Na+ channel activity in cultured renal (A6) epithelium: regulation by solution osmolarity.
1991,
Pubmed
,
Xenbase
Worrell,
CaMKII mediates stimulation of chloride conductance by calcium in T84 cells.
1991,
Pubmed
Yanase,
Adenosine 3',5'-cyclic monophosphate stimulates chloride secretion in A6 epithelia.
1986,
Pubmed
,
Xenbase
Yannoukakos,
Phosphorylation sites in human erythrocyte band 3 protein.
1991,
Pubmed
