Lopashov GV et al. (1997), Inductive capacity of living eye tissues from a...

XB-ART-16527

Differentiation 1997 May 01;614:237-42.

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Inductive capacity of living eye tissues from adult frogs.

Lopashov GV , Golubeva ON , Zviadadze KG .

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The aim of the present work has been to demonstrate the inducing capacity of living homogenous undamaged tissues from adult frogs. Tissues from the eyes of adult frogs Xenopus laevis: retina (R), pigmented epithelium (PE), lens epithelium (LE), and the forebrain (B) as a control, were tested for their inducing capacity using early gastrula ectoderm. To exclude the possibility of ad-mixture of inducing cells in early gastrula ectoderm the transfilter induction technique was used throughout. The results show that the tissues used in the cases of most intense induction tended to induce similar cell types: both R and PE induce R + PE together with adjoining neural cells and secondary lens cells. LE induces lentoids (L) and B cells induce neuroids. In each case epidermis surrounds the explants filled with ectomesenchyme (EM) and melanophores (M). Immunofluorescence reactions clarified the nature of lens cells. This discovery indicates that cells of adult tissues continue throughout life producing substances which are able to promote the appearance of cells of the same type during development. Probably they also serve to mediate interrelations between cells of these tissues, regulating the stability of their differentiation in the adult state, as well.

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	Fig. 1 Scheme of experiments with inductive influences of eye tissues of adult frogs on early gastrula ectoderm (EGE) of Xenopus laevis st. NF 10. A piece of retina (R), cells of pigmented epithelium (PE), a piece of lens epithelium (LE)
	Fig. 2 Relative distribution of frequencies of cell types evoked by different inducers in EGE. MS mesenchyme of any origin, including ectomesenchyme (EM).:
	Fig. 3 Inductions by R and PE in EGE. A Typical view of a living explant after 5 days of cultivation. B A case of lower-level induction by R; ectomesenchyme and melanophores are surrounded by thickened epidermis. C Induction of R with two small lenses near which a layer of PE surrounds R. D Case of almost typical eyes, induced by PE. Induction of R with a large lens; a layer of PE surrounds R; due to a small quantity of EM PE is poorly pigmented. Bar, 25 mμ (B, C); 40 mμ (D).:
	Fig. 4A–D Lenses induced by LE in EGE. A, B Typical lentoids (L). C, D The same lentoid: ordinary staining (C) and immunofluorescent reaction (D). Bar, 25 mμ (A, B); 15 mμ (C, D).:cgi&/