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Proc Natl Acad Sci U S A
1997 Jul 08;9414:7441-5. doi: 10.1073/pnas.94.14.7441.
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Photorepair mutants of Arabidopsis.
Jiang CZ
,
Yee J
,
Mitchell DL
,
Britt AB
.
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UV radiation induces two major DNA damage products, the cyclobutane pyrimidine dimer (CPD) and, at a lower frequency, the pyrimidine (6-4) pyrimidinone dimer (6-4 product). Although Escherichia coli and Saccharomyes cerevisiae produce a CPD-specific photolyase that eliminates only this class of dimer, Arabidopsis thaliana, Drosphila melanogaster, Crotalus atrox, and Xenopus laevis have recently been shown to photoreactivate both CPDs and 6-4 products. We describe the isolation and characterization of two new classes of mutants of Arabidopsis, termed uvr2 and uvr3, that are defective in the photoreactivation of CPDs and 6-4 products, respectively. We demonstrate that the CPD photolyase mutation is genetically linked to a DNA sequence encoding a type II (metazoan) CPD photolyase. In addition, we are able to generate plants in which only CPDs or 6-4 products are photoreactivated in the nuclear genome by exposing these mutants to UV light and then allowing them to repair one or the other class of dimers. This provides us with a unique opportunity to study the biological consequences of each of these two major UV-induced photoproducts in an intact living system.
Ahmad,
An enzyme similar to animal type II photolyases mediates photoreactivation in Arabidopsis.
1997, Pubmed
Ahmad,
An enzyme similar to animal type II photolyases mediates photoreactivation in Arabidopsis.
1997,
Pubmed
Ahmad,
HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor.
1993,
Pubmed
Britt,
A UV-sensitive mutant of Arabidopsis defective in the repair of pyrimidine-pyrimidinone(6-4) dimers.
1993,
Pubmed
Brunk,
Assay for nanogram quantities of DNA in cellular homogenates.
1979,
Pubmed
Chen,
A Light-Dependent Pathway for the Elimination of UV-Induced Pyrimidine (6-4) Pyrimidinone Photoproducts in Arabidopsis.
1994,
Pubmed
Chen,
Little or No Repair of Cyclobutyl Pyrimidine Dimers Is Observed in the Organellar Genomes of the Young Arabidopsis Seedling.
1996,
Pubmed
Donahue,
Transcript cleavage by RNA polymerase II arrested by a cyclobutane pyrimidine dimer in the DNA template.
1994,
Pubmed
Elledge,
Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.
1991,
Pubmed
Franklin,
The role of the (6-4) photoproduct in ultraviolet light-induced transition mutations in E. coli.
1986,
Pubmed
Hoeijmakers,
Nucleotide excision repair. II: From yeast to mammals.
1993,
Pubmed
Hoffman,
PHH1, a novel gene from Arabidopsis thaliana that encodes a protein similar to plant blue-light photoreceptors and microbial photolyases.
1996,
Pubmed
Kato,
Cloning of a marsupial DNA photolyase gene and the lack of related nucleotide sequences in placental mammals.
1994,
Pubmed
Kim,
Characterization of (6-4) photoproduct DNA photolyase.
1994,
Pubmed
Kim,
Purification and partial characterization of (6-4) photoproduct DNA photolyase from Xenopus laevis.
1996,
Pubmed
,
Xenbase
Landry,
An Arabidopsis photolyase mutant is hypersensitive to ultraviolet-B radiation.
1997,
Pubmed
Lawrence,
Mutagenesis induced by single UV photoproducts in E. coli and yeast.
1993,
Pubmed
Lin,
Association of flavin adenine dinucleotide with the Arabidopsis blue light receptor CRY1.
1995,
Pubmed
Livneh,
Replication of UV-irradiated single-stranded DNA by DNA polymerase III holoenzyme of Escherichia coli: evidence for bypass of pyrimidine photodimers.
1986,
Pubmed
Malhotra,
Putative blue-light photoreceptors from Arabidopsis thaliana and Sinapis alba with a high degree of sequence homology to DNA photolyase contain the two photolyase cofactors but lack DNA repair activity.
1995,
Pubmed
Mitchell,
Inhibition of transient gene expression in Chinese hamster ovary cells by cyclobutane dimers and (6-4) photoproducts in transfected ultraviolet-irradiated plasmid DNA.
1989,
Pubmed
Mitchell,
The biology of the (6-4) photoproduct.
1989,
Pubmed
Nelson,
Thymine-thymine dimer bypass by yeast DNA polymerase zeta.
1996,
Pubmed
Oda,
Arrested DNA replication in Xenopus and release by Escherichia coli mutagenesis proteins.
1996,
Pubmed
,
Xenbase
Painter,
Inhibition and recovery of DNA synthesis in human cells after exposure to ultraviolet light.
1985,
Pubmed
Pang,
UV-B-Inducible and Temperature-Sensitive Photoreactivation of Cyclobutane Pyrimidine Dimers in Arabidopsis thaliana.
1991,
Pubmed
Patrick,
Studies on thymine-derived UV photoproducts in DNA--I. Formation and biological role of pyrimidine adducts in DNA.
1977,
Pubmed
Protić-Sabljić,
One pyrimidine dimer inactivates expression of a transfected gene in xeroderma pigmentosum cells.
1985,
Pubmed
Selby,
Transcription preferentially inhibits nucleotide excision repair of the template DNA strand in vitro.
1990,
Pubmed
Taylor,
Solution-state structure of the Dewar pyrimidinone photoproduct of thymidylyl-(3'----5')-thymidine.
1988,
Pubmed
Taylor,
1H NMR assignment and melting temperature study of cis-syn and trans-syn thymine dimer containing duplexes of d(CGTATTATGC).d(GCATAATACG).
1990,
Pubmed
Thomas,
Replication of UV-irradiated DNA in human cell extracts: evidence for mutagenic bypass of pyrimidine dimers.
1993,
Pubmed
Todo,
Similarity among the Drosophila (6-4)photolyase, a human photolyase homolog, and the DNA photolyase-blue-light photoreceptor family.
1996,
Pubmed
Todo,
A new photoreactivating enzyme that specifically repairs ultraviolet light-induced (6-4)photoproducts.
1993,
Pubmed
Verma,
UV irradiation inhibits initiation of DNA replication from oriC in Escherichia coli.
1989,
Pubmed
Yasui,
A new class of DNA photolyases present in various organisms including aplacental mammals.
1994,
Pubmed