Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1997 Nov 25;9424:12839-44. doi: 10.1073/pnas.94.24.12839.
Show Gene links
Show Anatomy links
Anti-idiotype RNA selected with an anti-nuclear export signal antibody is actively transported in oocytes and inhibits Rev- and cap-dependent RNA export.
Hamm J
,
Huber J
,
Lührmann R
.
???displayArticle.abstract???
The anti-idiotype approach is based on the assumption that an antibody specific for a receptor-binding domain of a ligand could be structurally related to the receptor. Therefore, a structural mimic of a receptor-binding domain, selected with an anti-ligand antibody, might be a functional substrate for the receptor. This hypothesis was addressed here by generating antibodies recognizing the Rev-nuclear export signal (NES). A functional NES is required for active export, presumably by interacting directly or indirectly with the nuclear pore complex. Anti-NES antibodies were used to isolate RNA mimics of the NES peptide from combinatorial RNA libraries. The RNA-mimics are exported actively, block Rev-dependent export of a reporter RNA, and inhibit cap-dependent U1 snRNA export in Xenopus oocytes, properties previously reported for NES-peptide conjugates.
Cheong,
Solution structure of an unusually stable RNA tetraplex containing G- and U-quartet structures.
1992, Pubmed
Cheong,
Solution structure of an unusually stable RNA tetraplex containing G- and U-quartet structures.
1992,
Pubmed
Davis,
Antibody and HIV-1 gp120 recognition of CD4 undermines the concept of mimicry between antibodies and receptors.
1992,
Pubmed
Doudna,
Selection of an RNA molecule that mimics a major autoantigenic epitope of human insulin receptor.
1995,
Pubmed
Ellington,
In vitro selection of RNA molecules that bind specific ligands.
1990,
Pubmed
Emerman,
The rev gene product of the human immunodeficiency virus affects envelope-specific RNA localization.
1989,
Pubmed
Fedor,
A slippery grip.
1994,
Pubmed
Felber,
rev protein of human immunodeficiency virus type 1 affects the stability and transport of the viral mRNA.
1989,
Pubmed
Fischer,
Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA.
1994,
Pubmed
,
Xenbase
Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed
Gandini-Attardi,
Transfer RNA splicing endonuclease from Xenopus laevis.
1990,
Pubmed
,
Xenbase
Gaulton,
Idiotypic mimicry of biological receptors.
1986,
Pubmed
Gerace,
Nuclear export signals and the fast track to the cytoplasm.
1995,
Pubmed
Giver,
Selective optimization of the Rev-binding element of HIV-1.
1993,
Pubmed
Gold,
Diversity of oligonucleotide functions.
1995,
Pubmed
Görlich,
Nucleocytoplasmic transport.
1996,
Pubmed
Hamm,
Characterisation of antibody-binding RNAs selected from structurally constrained libraries.
1996,
Pubmed
Hamm,
The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal.
1990,
Pubmed
,
Xenbase
Hamm,
Monomethylated cap structures facilitate RNA export from the nucleus.
1990,
Pubmed
,
Xenbase
Kenan,
Exploring molecular diversity with combinatorial shape libraries.
1994,
Pubmed
Koepp,
A GTPase controlling nuclear trafficking: running the right way or walking RANdomly?
1996,
Pubmed
Malim,
The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA.
1989,
Pubmed
Michael,
A nuclear export signal in hnRNP A1: a signal-mediated, temperature-dependent nuclear protein export pathway.
1995,
Pubmed
Nigg,
Nucleocytoplasmic transport: signals, mechanisms and regulation.
1997,
Pubmed
Rhodes,
Telomere structure and function.
1995,
Pubmed
Sege,
Use of anti-idiotypic antibodies as cell-surface receptor probes.
1978,
Pubmed
Sundquist,
Evidence for interstrand quadruplex formation in the dimerization of human immunodeficiency virus 1 genomic RNA.
1993,
Pubmed
Tsai,
In vitro selection of an RNA epitope immunologically cross-reactive with a peptide.
1992,
Pubmed
Tuerk,
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.
1990,
Pubmed
Wen,
Identification of a signal for rapid export of proteins from the nucleus.
1995,
Pubmed
Zasloff,
Impaired nuclear transport of a human variant tRNAiMet.
1982,
Pubmed
,
Xenbase
