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Biochem J
1997 Dec 01;328 ( Pt 2):657-64. doi: 10.1042/bj3280657.
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Cloning, functional expression and dietary regulation of the mouse neutral and basic amino acid transporter (NBAT).
Segawa H
,
Miyamoto K
,
Ogura Y
,
Haga H
,
Morita K
,
Katai K
,
Tatsumi S
,
Nii T
,
Taketani Y
,
Takeda E
.
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The Na+-independent dibasic and neutral amino acid transporter NBAT is among the least hydrophobic of mammalian amino acid transporters. The transporter contains one to four transmembrane domains and induces amino acid transport activity via a b0,+-like system when expressed in Xenopus oocytes. However, the physiological role of NBAT remains unclear. Complementary DNA clones encoding mouse NBAT have now been isolated. The expression of mouse NBAT in Xenopus oocytes also induced an obligatory amino acid exchange activity similar to that of the b0,+-like system. The amount of NBAT mRNA in mouse kidney increased during postnatal development, consistent with the increase in renal cystine and dibasic transport activity. Dietary aspartate induced a marked increase in cystine transport via the b0,+ system in mouse ileum. A high-aspartate diet also increased the amount of NBAT mRNA in mouse ileum. In the ileum of mice fed on the aspartate diet, the extent of cystine transport was further increased by preloading brush border membrane vesicles with lysine. Hybrid depletion of NBAT mRNA from ileal polyadenylated RNA revealed that the increase in cystine transport activity induced by the high-aspartate diet, as measured in Xenopus oocytes, was attributable to NBAT. These results demonstrate that mouse NBAT has an important role in cystine transport.
Ahmed,
Sodium-independent currents of opposite polarity evoked by neutral and cationic amino acids in neutral and basic amino acid transporter cRNA-injected oocytes.
1995, Pubmed,
Xenbase
Ahmed,
Sodium-independent currents of opposite polarity evoked by neutral and cationic amino acids in neutral and basic amino acid transporter cRNA-injected oocytes.
1995,
Pubmed
,
Xenbase
Ahmed,
Electrogenic L-histidine transport in neutral and basic amino acid transporter (NBAT)-expressing Xenopus laevis oocytes. Evidence for two functionally distinct transport mechanisms induced by NBAT expression.
1997,
Pubmed
,
Xenbase
Bertran,
Expression cloning of a cDNA from rabbit kidney cortex that induces a single transport system for cystine and dibasic and neutral amino acids.
1992,
Pubmed
,
Xenbase
Bertran,
Expression cloning of a human renal cDNA that induces high affinity transport of L-cystine shared with dibasic amino acids in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Busch,
Opposite directed currents induced by the transport of dibasic and neutral amino acids in Xenopus oocytes expressing the protein rBAT.
1994,
Pubmed
,
Xenbase
Calonge,
Cystinuria caused by mutations in rBAT, a gene involved in the transport of cystine.
1994,
Pubmed
,
Xenbase
Calonge,
Genetic heterogeneity in cystinuria: the SLC3A1 gene is linked to type I but not to type III cystinuria.
1995,
Pubmed
Chillarón,
Obligatory amino acid exchange via systems bo,+-like and y+L-like. A tertiary active transport mechanism for renal reabsorption of cystine and dibasic amino acids.
1996,
Pubmed
,
Xenbase
Coady,
Electrogenic amino acid exchange via the rBAT transporter.
1994,
Pubmed
,
Xenbase
Erickson,
Regional expression and dietary regulation of rat small intestinal peptide and amino acid transporter mRNAs.
1995,
Pubmed
Ferraris,
Specific regulation of intestinal nutrient transporters by their dietary substrates.
1989,
Pubmed
Furriols,
rBAT, related to L-cysteine transport, is localized to the microvilli of proximal straight tubules, and its expression is regulated in kidney by development.
1993,
Pubmed
,
Xenbase
Gasparini,
Molecular genetics of cystinuria: identification of four new mutations and seven polymorphisms, and evidence for genetic heterogeneity.
1995,
Pubmed
Hisano,
The basic amino acid transporter (rBAT)-like immunoreactivity in paraventricular and supraoptic magnocellular neurons of the rat hypothalamus.
1996,
Pubmed
Kanai,
Expression of mRNA (D2) encoding a protein involved in amino acid transport in S3 proximal tubule.
1992,
Pubmed
,
Xenbase
Kim,
Transport of cationic amino acids by the mouse ecotropic retrovirus receptor.
1991,
Pubmed
,
Xenbase
Lee,
Cloning and chromosomal localization of a human kidney cDNA involved in cystine, dibasic, and neutral amino acid transport.
1993,
Pubmed
,
Xenbase
Magagnin,
Poly(A)+ RNA from rabbit intestinal mucosa induces b0,+ and y+ amino acid transport activities in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
McNamara,
Cystine uptake by rat renal brush-border vesicles.
1981,
Pubmed
Minami,
Inhibition of glucose absorption by phlorizin affects intestinal functions in rats.
1993,
Pubmed
Miyamoto,
Characterization of the rabbit intestinal fructose transporter (GLUT5).
1994,
Pubmed
,
Xenbase
Miyamoto,
Cloning and functional expression of a Na(+)-dependent phosphate co-transporter from human kidney: cDNA cloning and functional expression.
1995,
Pubmed
,
Xenbase
Miyamoto,
Mutations of the basic amino acid transporter gene associated with cystinuria.
1995,
Pubmed
,
Xenbase
Miyamoto,
Effects of truncation of the COOH-terminal region of a Na+-independent neutral and basic amino acid transporter on amino acid transport in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Pickel,
Ultrastructural localization of a neutral and basic amino acid transporter in rat kidney and intestine.
1993,
Pubmed
Pras,
Localization of a gene causing cystinuria to chromosome 2p.
1994,
Pubmed
Quackenbush,
Molecular cloning of complementary DNAs encoding the heavy chain of the human 4F2 cell-surface antigen: a type II membrane glycoprotein involved in normal and neoplastic cell growth.
1987,
Pubmed
Stein,
Comparison of different dietary amino acids as inducers of intestinal amino acid transport.
1987,
Pubmed
Tate,
Expression cloning of a Na(+)-independent neutral amino acid transporter from rat kidney.
1992,
Pubmed
,
Xenbase
Van Winkle,
Na+-independent transport of basic and zwitterionic amino acids in mouse blastocysts by a shared system and by processes which distinguish between these substrates.
1988,
Pubmed
Wang,
Oligomeric structure of a renal cystine transporter: implications in cystinuria.
1995,
Pubmed
,
Xenbase
Wells,
Cloning of a rat kidney cDNA that stimulates dibasic and neutral amino acid transport and has sequence similarity to glucosidases.
1992,
Pubmed
,
Xenbase
Yan,
Characterization of the promoter region of the gene for the rat neutral and basic amino acid transporter and chromosomal localization of the human gene.
1994,
Pubmed
