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Nucleic Acids Res
1998 Feb 01;263:703-9. doi: 10.1093/nar/26.3.703.
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The role of zinc finger linkers in p43 and TFIIIA binding to 5S rRNA and DNA.
Ryan RF
,
Darby MK
.
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Transcription factor IIIA (TFIIIA) and p43 zinc finger protein form distinct complexes with 5S ribosomal RNA in Xenopus oocytes. Additionally, TFIIIA binds the internal promoter of the 5S RNA gene and supports assembly of a transcription initiation complex. Both proteins have nine tandemly repeated zinc fingers with almost identical linker lengths between corresponding fingers, yet p43 has no detectable affinity for the 5S RNA gene. TFIIIA zinc fingers 1-3 are connected by highly conserved linkers, first identified in the Drosophila protein Krüppel, that are found in many DNA binding zinc finger proteins. To understand the role of these linkers in RNA and DNA binding we exchanged three TFIIIA linker amino acids with the equivalent amino acids from p43. The major effect of linker substitution is a 50-fold reduction in DNA specificity, concomitant with an 8-fold reduction in affinity. N-Terminal zinc fingers from either TFIIIA or p43 bind to multiple specific sites on 5S RNA that are resistant to competition by tRNA or poly(rA). This mode of RNA binding is unaffected by linker substitution. These data suggest that zinc finger linkers significantly facilitate the specificity of DNA binding.
Andreazzoli,
RNA binding properties and evolutionary conservation of the Xenopus multifinger protein Xfin.
1993, Pubmed,
Xenbase
Andreazzoli,
RNA binding properties and evolutionary conservation of the Xenopus multifinger protein Xfin.
1993,
Pubmed
,
Xenbase
Bailey,
Interpretation of nitrocellulose filter assays of protein--nucleic acid binding.
1979,
Pubmed
Brown,
The primary structure of transcription factor TFIIIA has 12 consecutive repeats.
1985,
Pubmed
,
Xenbase
Brüschweiler,
Long-range motional restrictions in a multidomain zinc-finger protein from anisotropic tumbling.
1995,
Pubmed
,
Xenbase
Caricasole,
RNA binding by the Wilms tumor suppressor zinc finger proteins.
1996,
Pubmed
Choo,
A role in DNA binding for the linker sequences of the first three zinc fingers of TFIIIA.
1993,
Pubmed
Clemens,
Molecular basis for specific recognition of both RNA and DNA by a zinc finger protein.
1993,
Pubmed
,
Xenbase
Clemens,
Relative contributions of the zinc fingers of transcription factor IIIA to the energetics of DNA binding.
1994,
Pubmed
,
Xenbase
Darby,
Differential binding of zinc fingers from Xenopus TFIIIA and p43 to 5S RNA and the 5S RNA gene.
1992,
Pubmed
,
Xenbase
Del Rio,
The function of individual zinc fingers in sequence-specific DNA recognition by transcription factor IIIA.
1993,
Pubmed
,
Xenbase
Del Río,
High yield purification of active transcription factor IIIA expressed in E. coli.
1991,
Pubmed
,
Xenbase
Fairall,
The crystal structure of a two zinc-finger peptide reveals an extension to the rules for zinc-finger/DNA recognition.
1993,
Pubmed
Fried,
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
1981,
Pubmed
Friesen,
Phage display of RNA binding zinc fingers from transcription factor IIIA.
1997,
Pubmed
,
Xenbase
Hansen,
Dissection of the DNA-binding domain of Xenopus laevis TFIIIA. Quantitative DNase I footprinting analysis of specific complexes between a 5 S RNA gene fragment and N-terminal fragments of TFIIIA containing three, four or five zinc-finger domains.
1993,
Pubmed
,
Xenbase
Joho,
A finger protein structurally similar to TFIIIA that binds exclusively to 5S RNA in Xenopus.
1990,
Pubmed
,
Xenbase
Klocke,
The FAR domain defines a new Xenopus laevis zinc finger protein subfamily with specific RNA homopolymer binding activity.
1994,
Pubmed
,
Xenbase
Köster,
Structure, expression and in vitro functional characterization of a novel RNA binding zinc finger protein from Xenopus.
1991,
Pubmed
,
Xenbase
Larsson,
Subnuclear localization of WT1 in splicing or transcription factor domains is regulated by alternative splicing.
1995,
Pubmed
Liao,
Specific interaction of the first three zinc fingers of TFIIIA with the internal control region of the Xenopus 5 S RNA gene.
1992,
Pubmed
,
Xenbase
Liao,
1H, 15N and 13C resonance assignments for the first three zinc fingers of transcription factor IIIA.
1994,
Pubmed
,
Xenbase
Mao,
A position-dependent transcription-activating domain in TFIIIA.
1993,
Pubmed
,
Xenbase
McBryant,
Interaction of the RNA binding fingers of Xenopus transcription factor IIIA with specific regions of 5 S ribosomal RNA.
1995,
Pubmed
,
Xenbase
Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Nakaseko,
Adjacent zinc-finger motifs in multiple zinc-finger peptides from SWI5 form structurally independent, flexibly linked domains.
1992,
Pubmed
Pavletich,
Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A.
1991,
Pubmed
Pavletich,
Crystal structure of a five-finger GLI-DNA complex: new perspectives on zinc fingers.
1993,
Pubmed
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Rollins,
Role of TFIIIA zinc fingers in vivo: analysis of single-finger function in developing Xenopus embryos.
1993,
Pubmed
,
Xenbase
Romaniuk,
Characterization of the equilibrium binding of Xenopus transcription factor IIIA to the 5 S RNA gene.
1990,
Pubmed
,
Xenbase
Sands,
Two zinc finger proteins from Xenopus laevis bind the same region of 5S RNA but with different nuclease protection patterns.
1991,
Pubmed
,
Xenbase
Schuh,
A conserved family of nuclear proteins containing structural elements of the finger protein encoded by Krüppel, a Drosophila segmentation gene.
1986,
Pubmed
,
Xenbase
Schägger,
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
1987,
Pubmed
Setzer,
Functional interactions between the zinc fingers of Xenopus transcription factor IIIA during 5S rRNA binding.
1996,
Pubmed
,
Xenbase
Smith,
Structural elements in the N-terminal half of transcription factor IIIA required for factor binding to the 5S RNA gene internal control region.
1991,
Pubmed
,
Xenbase
Studier,
Use of T7 RNA polymerase to direct expression of cloned genes.
1990,
Pubmed
Theunissen,
RNA and DNA binding zinc fingers in Xenopus TFIIIA.
1992,
Pubmed
,
Xenbase
Vandeyar,
A simple and rapid method for the selection of oligodeoxynucleotide-directed mutants.
1988,
Pubmed
Vrana,
Mapping functional regions of transcription factor TFIIIA.
1988,
Pubmed
,
Xenbase
Zang,
Effects of zinc finger mutations on the nucleic acid binding activities of Xenopus transcription factor IIIA.
1995,
Pubmed
,
Xenbase
Zang,
Characterization of the 5 S RNA binding activity of Xenopus zinc finger protein p43.
1995,
Pubmed
,
Xenbase
Zwieb,
Absence of substantial bending in Xenopus laevis transcription factor IIIA-DNA complexes.
1990,
Pubmed
,
Xenbase