Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nucleic Acids Res
1998 Feb 15;264:1019-25. doi: 10.1093/nar/26.4.1019.
Show Gene links
Show Anatomy links
Transcription initiation from a poly(dA) tract.
Shain DH
,
Zuber MX
,
Neuman T
.
???displayArticle.abstract???
The mammalian ME1 gene encodes a non-tissue-specific, helix-loop-helix transcription factor that is enriched in morphogenetically active regions during development. Regulation of mouse ME1 gene expression is controlled by a novel initiator (ME1 Inr) that promotes transcription from the center of a 13 bp poly(dA) tract. We show here that the ME1 Inr autonomously directs initiation from the poly(dA) tract both in vitro and in vivo. This transcription was dependent upon two protein complexes; MBPalpha, which associated directly with the poly(dA) tract, and MBPbeta, which introduced an approximately 60 degrees bend immediately downstream of the poly(dA) tract. The MBPalpha and MBPbeta binding sites were strikingly conserved in homologous DNA from several mammalian species and the frog Xenopus laevis. These results suggest that the ME1 Inr constitutes a robust nucleation site that promotes transcription initiation in the absence of conventional promoter elements.
Becker,
DNA loops induced by cooperative binding of transcriptional activator proteins and preinitiation complexes.
1995, Pubmed
Becker,
DNA loops induced by cooperative binding of transcriptional activator proteins and preinitiation complexes.
1995,
Pubmed
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase
Boettcher,
Transcription initiation and nuclease-sensitive sites upstream of the epsilon-globin gene in K562 cells are related to poly (dA).poly (dT) sequences.
1990,
Pubmed
Graham,
A new technique for the assay of infectivity of human adenovirus 5 DNA.
1973,
Pubmed
Horikoshi,
Transcription factor TFIID induces DNA bending upon binding to the TATA element.
1992,
Pubmed
Huber,
Retinoblastoma protein reverses DNA bending by transcription factor E2F.
1994,
Pubmed
Iyer,
Poly(dA:dT), a ubiquitous promoter element that stimulates transcription via its intrinsic DNA structure.
1995,
Pubmed
Kahn,
DNA bending in transcription initiation.
1993,
Pubmed
Kim,
Bending of DNA by gene-regulatory proteins: construction and use of a DNA bending vector.
1989,
Pubmed
Klein,
Tissue-specific RNA splicing generates an ankyrin-like domain that affects the dimerization and DNA-binding properties of a bHLH protein.
1993,
Pubmed
Kunkel,
Nucleosomes will not form on double-stranded RNa or over poly(dA).poly(dT) tracts in recombinant DNA.
1981,
Pubmed
Martinez,
TATA-binding protein-associated factor(s) in TFIID function through the initiator to direct basal transcription from a TATA-less class II promoter.
1994,
Pubmed
Nardulli,
Estrogen receptor-induced DNA bending: orientation of the bend and replacement of an estrogen response element with an intrinsic DNA bending sequence.
1995,
Pubmed
Nelson,
The structure of an oligo(dA).oligo(dT) tract and its biological implications.
,
Pubmed
Neuman,
ME1 and GE1: basic helix-loop-helix transcription factors expressed at high levels in the developing nervous system and in morphogenetically active regions.
1993,
Pubmed
Parvin,
Pre-bending of a promoter sequence enhances affinity for the TATA-binding factor.
1995,
Pubmed
Proudfoot,
3' non-coding region sequences in eukaryotic messenger RNA.
1976,
Pubmed
Prunell,
Nucleosome reconstitution on plasmid-inserted poly(dA) . poly(dT).
1982,
Pubmed
Pugh,
Mechanism of transcriptional activation by Sp1: evidence for coactivators.
1990,
Pubmed
Pérez-Martín,
Promoters responsive to DNA bending: a common theme in prokaryotic gene expression.
1994,
Pubmed
Shain,
A novel initiator regulates expression of the nontissue-specific helix-loop-helix gene ME1.
1995,
Pubmed
Shain,
Embryonic expression and evolution of duplicated E-protein genes in Xenopus laevis: parallels with ancestral E-protein genes.
1997,
Pubmed
,
Xenbase
Sharrocks,
DNA bending in the ternary nucleoprotein complex at the c-fos promoter.
1995,
Pubmed
Smale,
The "initiator" as a transcription control element.
1989,
Pubmed
Smale,
Transcriptional activation by Sp1 as directed through TATA or initiator: specific requirement for mammalian transcription factor IID.
1990,
Pubmed
Usheva,
TATA-binding protein-independent initiation: YY1, TFIIB, and RNA polymerase II direct basal transcription on supercoiled template DNA.
1994,
Pubmed
Wiley,
Functional binding of the "TATA" box binding component of transcription factor TFIID to the -30 region of TATA-less promoters.
1992,
Pubmed
Winter,
A DNA binding protein that recognizes oligo(dA).oligo(dT) tracts.
1989,
Pubmed
Wu,
The locus of sequence-directed and protein-induced DNA bending.
,
Pubmed
Zenzie-Gregory,
Similar mechanisms for transcription initiation mediated through a TATA box or an initiator element.
1992,
Pubmed
Zhou,
Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter.
1992,
Pubmed
Zinkel,
DNA bend direction by phase sensitive detection.
,
Pubmed
