Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
A mutation affecting dihydropyridine-sensitive current levels and activation kinetics in Drosophila muscle and mammalian heart calcium channels.
Ren D
,
Xu H
,
Eberl DF
,
Chopra M
,
Hall LM
.
???displayArticle.abstract???
The Dmca1D gene encodes a Drosophila calcium channel alpha1 subunit. We describe the first functional characterization of a mutation in this gene. This alpha1 subunit mediates the dihydropyridine-sensitive calcium channel current in larval muscle but does not contribute to the amiloride-sensitive current in that tissue. A mutation, which changes a highly conserved Cys to Tyr in transmembrane domain IS1, identifies a residue important for channel function not only in Drosophila muscle but also in mammalian cardiac channels. In both cases, mutations in this Cys residue slow channel activation and reduce expressed currents. Amino acid substitutions at this Cys position in the cardiac alpha1 subunit show that the size of the side chain, rather than its ability to form disulfide bonds, affects channel activation.
Adams,
Ca2+ current activation rate correlates with alpha 1 subunit density.
1996, Pubmed
Adams,
Ca2+ current activation rate correlates with alpha 1 subunit density.
1996,
Pubmed
Ashburner,
The genetics of a small autosomal region of Drosophila melanogaster containing the structural gene for alcohol dehydrogenase. VII. Characterization of the region around the snail and cactus loci.
1990,
Pubmed
Catterall,
Receptor sites for Ca2+ channel antagonists.
1992,
Pubmed
Catterall,
Cellular and molecular biology of voltage-gated sodium channels.
1992,
Pubmed
Catterall,
Structure and function of voltage-gated ion channels.
1995,
Pubmed
Cormack,
Directed mutagenesis using the polymerase chain reaction.
2001,
Pubmed
Dargent,
Down-regulation of voltage-dependent sodium channels initiated by sodium influx in developing neurons.
1990,
Pubmed
Dargent,
Sodium channel internalization in developing neurons.
1994,
Pubmed
Dunlap,
Exocytotic Ca2+ channels in mammalian central neurons.
1995,
Pubmed
Eberl,
Genetic and developmental characterization of Dmca1D, a calcium channel alpha1 subunit gene in Drosophila melanogaster.
1998,
Pubmed
Ellis,
Sequence and expression of mRNAs encoding the alpha 1 and alpha 2 subunits of a DHP-sensitive calcium channel.
1988,
Pubmed
García,
Role of S4 segments and the leucine heptad motif in the activation of an L-type calcium channel.
1997,
Pubmed
Gielow,
Resolution and pharmacological analysis of the voltage-dependent calcium channels of Drosophila larval muscles.
1995,
Pubmed
Heinemann,
Calcium channel characteristics conferred on the sodium channel by single mutations.
1992,
Pubmed
,
Xenbase
Hofmann,
Molecular basis for Ca2+ channel diversity.
1994,
Pubmed
Hui,
Molecular cloning of multiple subtypes of a novel rat brain isoform of the alpha 1 subunit of the voltage-dependent calcium channel.
1991,
Pubmed
Jan,
Properties of the larval neuromuscular junction in Drosophila melanogaster.
1976,
Pubmed
Larsson,
Transmembrane movement of the shaker K+ channel S4.
1996,
Pubmed
,
Xenbase
Leung,
Characterization of single calcium channels in Drosophila embryonic nerve and muscle cells.
1991,
Pubmed
Maricq,
Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel.
1991,
Pubmed
,
Xenbase
Mikami,
Primary structure and functional expression of the cardiac dihydropyridine-sensitive calcium channel.
1989,
Pubmed
,
Xenbase
Mori,
Primary structure and functional expression from complementary DNA of a brain calcium channel.
1991,
Pubmed
,
Xenbase
Nakai,
Critical roles of the S3 segment and S3-S4 linker of repeat I in activation of L-type calcium channels.
1994,
Pubmed
Neely,
Potentiation by the beta subunit of the ratio of the ionic current to the charge movement in the cardiac calcium channel.
1993,
Pubmed
,
Xenbase
Offord,
Electrical activity, cAMP, and cytosolic calcium regulate mRNA encoding sodium channel alpha subunits in rat muscle cells.
1989,
Pubmed
Pelzer,
Diversity and novel pharmacological properties of Ca2+ channels in Drosophila brain membranes.
1989,
Pubmed
Perez-Reyes,
Cloning and expression of a cardiac/brain beta subunit of the L-type calcium channel.
1992,
Pubmed
,
Xenbase
Pongs,
Shaker encodes a family of putative potassium channel proteins in the nervous system of Drosophila.
1988,
Pubmed
Pragnell,
Cloning and tissue-specific expression of the brain calcium channel beta-subunit.
1991,
Pubmed
Pragnell,
Calcium channel beta-subunit binds to a conserved motif in the I-II cytoplasmic linker of the alpha 1-subunit.
1994,
Pubmed
Schreibmayer,
Voltage clamping of Xenopus laevis oocytes utilizing agarose-cushion electrodes.
1994,
Pubmed
,
Xenbase
Singh,
Complete separation of four potassium currents in Drosophila.
1989,
Pubmed
Smith,
A Drosophila calcium channel alpha1 subunit gene maps to a genetic locus associated with behavioral and visual defects.
1996,
Pubmed
Soong,
Structure and functional expression of a member of the low voltage-activated calcium channel family.
1993,
Pubmed
Stewart,
Improved stability of Drosophila larval neuromuscular preparations in haemolymph-like physiological solutions.
1994,
Pubmed
Tanabe,
Repeat I of the dihydropyridine receptor is critical in determining calcium channel activation kinetics.
1991,
Pubmed
Tanabe,
Primary structure of the receptor for calcium channel blockers from skeletal muscle.
,
Pubmed
Tang,
Molecular localization of ion selectivity sites within the pore of a human L-type cardiac calcium channel.
1993,
Pubmed
Tsien,
Molecular diversity of voltage-dependent Ca2+ channels.
1991,
Pubmed
Varadi,
Molecular determinants of Ca2+ channel function and drug action.
1995,
Pubmed
Wang,
Chimeric L-type Ca2+ channels expressed in Xenopus laevis oocytes reveal role of repeats III and IV in activation gating.
1995,
Pubmed
,
Xenbase
Wei,
Heterologous regulation of the cardiac Ca2+ channel alpha 1 subunit by skeletal muscle beta and gamma subunits. Implications for the structure of cardiac L-type Ca2+ channels.
1991,
Pubmed
,
Xenbase
Williams,
Structure and functional expression of an omega-conotoxin-sensitive human N-type calcium channel.
1992,
Pubmed
Wu,
Voltage clamp analysis of membrane currents in larval muscle fibers of Drosophila: alteration of potassium currents in Shaker mutants.
1985,
Pubmed
Yang,
Molecular basis of charge movement in voltage-gated sodium channels.
1996,
Pubmed
Yang,
Molecular determinants of Ca2+ selectivity and ion permeation in L-type Ca2+ channels.
1993,
Pubmed
,
Xenbase
Zheng,
Cloning and characterization of a calcium channel alpha 1 subunit from Drosophila melanogaster with similarity to the rat brain type D isoform.
1995,
Pubmed