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The U14 snoRNA is required for 2'-O-methylation of the pre-18S rRNA in Xenopus oocytes.
Dunbar DA
,
Baserga SJ
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We have studied the role of the U14 small nucleolar RNA (snoRNA) in pre-rRNA methylation and processing in Xenopus oocytes. Depletion of U14 in Xenopus oocytes was achieved by co-injecting two nonoverlapping antisense oligonucleotides. Focusing on the earliest precursor, depletion experiments revealed that the U14 snoRNA is essential for 2'-O-ribose methylation at nt 427 of the 18S rRNA. Injection of U14-depleted oocytes with specific U14 mutant snoRNAs indicated that conserved domain B, but not domain A, of U14 is required for the methylation reaction. When the effect of U14 on pre-rRNA processing is assayed, we find only modest effects on 18S rRNA levels, and no effect on the type or accumulation of 18S precursors, suggesting a role for U14 in a step in ribosome biogenesis other than cleavage of the pre-rRNA. Xenopus U14 is, therefore, a Box C/D fibrillarin-associated snoRNA that is required for site-specific 2'-O-ribose methylation of pre-rRNA.
Anderson,
Synthesis of heterogeneous nuclear RNA in full-grown oocytes of Xenopus laevis (Daudin).
1977, Pubmed,
Xenbase
Anderson,
Synthesis of heterogeneous nuclear RNA in full-grown oocytes of Xenopus laevis (Daudin).
1977,
Pubmed
,
Xenbase
Balakin,
The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions.
1996,
Pubmed
Baserga,
An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs.
1991,
Pubmed
Cavaillé,
Targeted ribose methylation of RNA in vivo directed by tailored antisense RNA guides.
1996,
Pubmed
Dunbar,
The U18 snRNA is not essential for pre-rRNA processing in Xenopus laevis.
1996,
Pubmed
,
Xenbase
Enright,
5'ETS rRNA processing facilitated by four small RNAs: U14, E3, U17, and U3.
1996,
Pubmed
Ganot,
Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs.
1997,
Pubmed
Hughes,
Depletion of U3 small nucleolar RNA inhibits cleavage in the 5' external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA.
1991,
Pubmed
Inoue,
Sequence-dependent hydrolysis of RNA using modified oligonucleotide splints and RNase H.
1987,
Pubmed
Kass,
The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing.
1990,
Pubmed
Kiss-László,
Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs.
1996,
Pubmed
Kumar,
Ribosome synthesis in Tetrahymena pyriformis.
1970,
Pubmed
Lapham,
The position of site-directed cleavage of RNA using RNase H and 2'-O-methyl oligonucleotides is dependent on the enzyme source.
1997,
Pubmed
Lapham,
RNase H cleavage for processing of in vitro transcribed RNA for NMR studies and RNA ligation.
1996,
Pubmed
Leader,
Molecular characterisation of plant U14 small nucleolar RNA genes: closely linked genes are transcribed as polycistronic U14 transcripts.
1994,
Pubmed
Li,
Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae.
1990,
Pubmed
Li,
U14 function in Saccharomyces cerevisiae can be provided by large deletion variants of yeast U14 and hybrid mouse-yeast U14 RNAs.
1992,
Pubmed
Liang,
U14 base-pairs with 18S rRNA: a novel snoRNA interaction required for rRNA processing.
1995,
Pubmed
Liang,
The rRNA-processing function of the yeast U14 small nucleolar RNA can be rescued by a conserved RNA helicase-like protein.
1997,
Pubmed
Lindahl,
A new rRNA processing mutant of Saccharomyces cerevisiae.
1992,
Pubmed
Liu,
Mouse U14 snRNA is encoded in an intron of the mouse cognate hsc70 heat shock gene.
1990,
Pubmed
Maden,
The numerous modified nucleotides in eukaryotic ribosomal RNA.
1990,
Pubmed
Maden,
Eukaryotic ribosomal RNA: the recent excitement in the nucleotide modification problem.
1997,
Pubmed
Maxwell,
The small nucleolar RNAs.
1995,
Pubmed
Mishra,
Three small nucleolar RNAs that are involved in ribosomal RNA precursor processing.
1997,
Pubmed
,
Xenbase
Morrissey,
Yeast snR30 is a small nucleolar RNA required for 18S rRNA synthesis.
1993,
Pubmed
Morrissey,
U14 small nucleolar RNA makes multiple contacts with the pre-ribosomal RNA.
1997,
Pubmed
Mougey,
The terminal balls characteristic of eukaryotic rRNA transcription units in chromatin spreads are rRNA processing complexes.
1993,
Pubmed
,
Xenbase
Mougey,
A U3 small nuclear ribonucleoprotein-requiring processing event in the 5' external transcribed spacer of Xenopus precursor rRNA.
1993,
Pubmed
,
Xenbase
Ni,
Small nucleolar RNAs direct site-specific synthesis of pseudouridine in ribosomal RNA.
1997,
Pubmed
Nicoloso,
Intron-encoded, antisense small nucleolar RNAs: the characterization of nine novel species points to their direct role as guides for the 2'-O-ribose methylation of rRNAs.
1996,
Pubmed
Pan,
Assembly of functional U1 and U2 human-amphibian hybrid snRNPs in Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Peculis,
Disruption of U8 nucleolar snRNA inhibits 5.8S and 28S rRNA processing in the Xenopus oocyte.
1993,
Pubmed
,
Xenbase
Peculis,
Ribosomal RNA: small nucleolar RNAs make their mark.
1996,
Pubmed
Rebagliati,
Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activity.
1987,
Pubmed
,
Xenbase
Samarsky,
An essential domain in Saccharomyces cerevisiae U14 snoRNA is absent in vertebrates, but conserved in other yeasts.
1996,
Pubmed
Savino,
In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing.
1990,
Pubmed
,
Xenbase
Schmitt,
Nuclear RNase MRP is required for correct processing of pre-5.8S rRNA in Saccharomyces cerevisiae.
1993,
Pubmed
Shuai,
A temperature sensitive mutant of Saccharomyces cerevisiae defective in pre-rRNA processing.
1991,
Pubmed
Smith,
Sno storm in the nucleolus: new roles for myriad small RNPs.
1997,
Pubmed
Tollervey,
Small nucleolar RNAs guide ribosomal RNA methylation.
1996,
Pubmed
Trapman,
Maturation of ribosomes in yeast. I Kinetic analysis by labelling of high molecular weight rRNA species.
1976,
Pubmed
Tycowski,
Requirement for intron-encoded U22 small nucleolar RNA in 18S ribosomal RNA maturation.
1994,
Pubmed
,
Xenbase
Tycowski,
A small nucleolar RNA requirement for site-specific ribose methylation of rRNA in Xenopus.
1996,
Pubmed
,
Xenbase
Udem,
Ribosomal RNA synthesis in Saccharomyces cerevisiae.
1972,
Pubmed
Venema,
Processing of pre-ribosomal RNA in Saccharomyces cerevisiae.
1995,
Pubmed
Warner,
Ribosome formation in HeLa cells in the absence of protein synthesis.
1966,
Pubmed
Watkins,
Elements essential for processing intronic U14 snoRNA are located at the termini of the mature snoRNA sequence and include conserved nucleotide boxes C and D.
1996,
Pubmed
,
Xenbase
Xia,
Intronic U14 snoRNAs of Xenopus laevis are located in two different parent genes and can be processed from their introns during early oogenesis.
1995,
Pubmed
,
Xenbase
Xia,
Identification of specific nucleotide sequences and structural elements required for intronic U14 snoRNA processing.
1997,
Pubmed
Yu,
A new method for detecting sites of 2'-O-methylation in RNA molecules.
1997,
Pubmed
,
Xenbase
Zafarullah,
Molecular cloning and characterization of a constitutively expressed heat-shock-cognate hsc71 gene from rainbow trout.
1992,
Pubmed
Zagorski,
Characterization of an SNR gene locus in Saccharomyces cerevisiae that specifies both dispensible and essential small nuclear RNAs.
1988,
Pubmed
