Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biochem J
1998 Mar 15;330 ( Pt 3):1223-7. doi: 10.1042/bj3301223.
Show Gene links
Show Anatomy links
The gene structure and promoter sequence of mouse hyaluronan synthase 1.
Yamada Y
,
Itano N
,
Zako M
,
Yoshida M
,
Lenas P
,
Niimi A
,
Ueda M
,
Kimata K
.
???displayArticle.abstract???
The structure and organization of mouse hyaluronan synthase 1 gene, HAS1 were determined by direct sequencing of lambda phage clones carrying the entire gene and by application of the long and accurate (LA)-PCR method to amplify regions encompassing the exon-intron boundaries and all of the exons. This gene spans about 11kb of genomic DNA and consists of 5 exons and 4 introns. A similarity in the exon-intron organization was found between the genes of mouse HAS1 and Xenopus laevis DG42 which was recently identified as Xenopus hyaluronan synthase. The transcription initiation site was determined by rapid amplification of the cDNA ends (5'-RACE). Position +1 is located 55 nucleotides upstream of the ATG initiation codon. The promoter region of the HAS1 gene has no typical TATA box, but contains a CCAAT box located 190 nucleotides upstream of the transcription initiation site. Further analysis of 1.4 kb of the 5' flanking region revealed several potential binding motifs for transcription factors. This information about the gene structure may be useful for further studies on the promoter activity.
Campbell,
p53 mutations are common and early events that precede tumor invasion in squamous cell neoplasia of the skin.
1993, Pubmed
Campbell,
p53 mutations are common and early events that precede tumor invasion in squamous cell neoplasia of the skin.
1993,
Pubmed
Connor,
DNA binding and bending properties of the post-meiotically expressed Sry-related protein Sox-5.
1994,
Pubmed
Dailey,
Distinct transcription factors bind specifically to two regions of the human histone H4 promoter.
1986,
Pubmed
DeAngelis,
Molecular cloning, identification, and sequence of the hyaluronan synthase gene from group A Streptococcus pyogenes.
1993,
Pubmed
,
Xenbase
DeAngelis,
Yeast-derived recombinant DG42 protein of Xenopus can synthesize hyaluronan in vitro.
1996,
Pubmed
,
Xenbase
Denny,
An SRY-related gene expressed during spermatogenesis in the mouse encodes a sequence-specific DNA-binding protein.
1992,
Pubmed
Edwards,
Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.
1991,
Pubmed
Foster,
Campomelic dysplasia and autosomal sex reversal caused by mutations in an SRY-related gene.
1994,
Pubmed
Frohman,
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
1988,
Pubmed
Goa,
Hyaluronic acid. A review of its pharmacology and use as a surgical aid in ophthalmology, and its therapeutic potential in joint disease and wound healing.
1994,
Pubmed
Gubbay,
A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes.
1990,
Pubmed
Hall,
Overexpression of the hyaluronan receptor RHAMM is transforming and is also required for H-ras transformation.
1995,
Pubmed
Hall,
Hyaluronan: RHAMM mediated cell locomotion and signaling in tumorigenesis.
1995,
Pubmed
Harada,
Anti-oncogenic and oncogenic potentials of interferon regulatory factors-1 and -2.
1993,
Pubmed
Imagawa,
Transcription factor AP-2 mediates induction by two different signal-transduction pathways: protein kinase C and cAMP.
1987,
Pubmed
Itano,
Expression cloning and molecular characterization of HAS protein, a eukaryotic hyaluronan synthase.
1996,
Pubmed
,
Xenbase
Itano,
Molecular cloning of human hyaluronan synthase.
1996,
Pubmed
,
Xenbase
Kimata,
Increased synthesis of hyaluronic acid by mouse mammary carcinoma cell variants with high metastatic potential.
1983,
Pubmed
Knudson,
The role and regulation of tumour-associated hyaluronan.
1989,
Pubmed
Lassar,
MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancer.
1989,
Pubmed
Laurent,
Hyaluronan.
1992,
Pubmed
Lesley,
CD44 and its interaction with extracellular matrix.
1993,
Pubmed
Levine,
The p53 tumour suppressor gene.
1991,
Pubmed
Meyer,
Cells expressing the DG42 gene from early Xenopus embryos synthesize hyaluronan.
1996,
Pubmed
,
Xenbase
Mukhopadhyay,
Functional inactivation of p53 by antisense RNA induces invasive ability of lung carcinoma cells and downregulates cytokeratin synthesis.
1996,
Pubmed
Rosa,
Accumulation and decay of DG42 gene products follow a gradient pattern during Xenopus embryogenesis.
1988,
Pubmed
,
Xenbase
Shapiro,
RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression.
1987,
Pubmed
Shyjan,
Functional cloning of the cDNA for a human hyaluronan synthase.
1996,
Pubmed
,
Xenbase
Spicer,
Molecular cloning and characterization of a putative mouse hyaluronan synthase.
1996,
Pubmed
,
Xenbase
Spicer,
Molecular cloning and characterization of a cDNA encoding the third putative mammalian hyaluronan synthase.
1997,
Pubmed
,
Xenbase
Tomida,
Effects of adenosine 3':5'-cyclic monophosphate and serum on synthesis of hyaluronic acid in confluent rat fibroblasts.
1977,
Pubmed
Tsukiyama,
ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor.
1994,
Pubmed
Turley,
The role of a cell-associated hyaluronan-binding protein in fibroblast behaviour.
1989,
Pubmed
Vogelstein,
p53 function and dysfunction.
1992,
Pubmed
Watanabe,
Molecular identification of a putative human hyaluronan synthase.
1996,
Pubmed
,
Xenbase
Weigel,
Hyaluronan synthases.
1997,
Pubmed
,
Xenbase
Weintraub,
Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD.
1989,
Pubmed
Williams,
Analysis of the DNA-binding and activation properties of the human transcription factor AP-2.
1991,
Pubmed
Yamamoto,
Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB.
1988,
Pubmed
Yim,
IFN regulatory factor-1 gene transfer into an aggressive, nonimmunogenic sarcoma suppresses the malignant phenotype and enhances immunogenicity in syngeneic mice.
1997,
Pubmed
Yoneda,
Hyaluronic acid modulates proliferation of mouse dermal fibroblasts in culture.
1988,
Pubmed