Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-14967
Neuroreport 1998 Apr 20;96:1089-92. doi: 10.1097/00001756-199804200-00024.
Show Gene links Show Anatomy links

Gamma subunit dependent modulation by nitric oxide (NO) in recombinant GABAA receptor.

Fukami S , Uchida I , Mashimo T , Takenoshita M , Yoshiya I .


???displayArticle.abstract???
The effect of nitric oxide (NO) on GABA induced Cl- current of recombinant GABAA receptors was studied. Either alpha 1 beta 2 gamma 2s or alpha 1 beta 2 subunit mRNAs synthesized from cDNA of mouse brain were injected into Xenopus oocytes and functional GABAA receptors were expressed. GABA-induced Cl- current was measured with the two electrode voltage clamp technique. The NO donor NOC-18 reduced the GABA-induced Cl- current in the alpha 1 beta 2 gamma 2s subunit receptor in a dose-dependent manner. In alpha 1,beta 2 subunit receptor, NOC-18 had no effects on GABA-induced currents at low concentrations but showed potentiation at high concentration. These effects were antagonized by the NO extinguisher, carboxy-PTIO. The cGMP analogue 8-Br-cGMP failed to induce NO-like effects. NO directly acts at the GABAA receptor and the gamma 2s subunit is involved in its action.

???displayArticle.pubmedLink??? 9601673
???displayArticle.link??? Neuroreport


Species referenced: Xenopus
Genes referenced: gabarap noct uqcc6