XB-ART-1440
J Biol Chem
2005 Oct 28;28043:36150-7. doi: 10.1074/jbc.M507706200.
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Identification and functional characterization of a voltage-gated chloride channel and its novel splice variant in taste bud cells.
Huang L
,
Cao J
,
Wang H
,
Vo LA
,
Brand JG
.
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Taste bud cells are epithelial cells with neuronal properties. Voltage-dependent ion channels have been physiologically described in these cells. Here, we report the molecular identification and functional characterization of a voltage-gated chloride channel (ClC-4) and its novel splice variant (ClC-4A) from taste bud cells. ClC-4A skipped an exon near its 5'-end, incurring the loss of 60 amino acids at the N terminus. In situ hybridization and immunohistochemistry localized these two channels' transcripts and proteins to a subset of taste bud cells. Electrophysiological recordings of the heterologously expressed channels in Xenopus oocytes showed that ClC-4 and ClC-4A have opposite sensitivity to pH and unique ion selectivity. The chloride channel blockers niflumic acid and 5-nitro-2-(3-phenylpropylamino)benzoic acid had a slight or no inhibitory effect on the conductance of ClC-4, but both blockers inhibited ClC-4A, suggesting that ClC-4A is a candidate channel for an acid-induced 5-nitro-2-(3-phenylpropylamino)benzoic acid-sensitive current. Furthermore, these two channels may play a role in bitter-, sweet-, and umami-mediated taste transmission by regulating transmitter uptake into synaptic vesicles.
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References [+] :
Adler,
A novel family of mammalian taste receptors.
2000, Pubmed
Adler, A novel family of mammalian taste receptors. 2000, Pubmed
Adler, Evidence of evolutionary up-regulation of the single active X chromosome in mammals based on Clc4 expression levels in Mus spretus and Mus musculus. 1997, Pubmed
Avenet, Noninvasive recording of receptor cell action potentials and sustained currents from single taste buds maintained in the tongue: the response to mucosal NaCl and amiloride. 1991, Pubmed
Bachmanov, Positional cloning of the mouse saccharin preference (Sac) locus. 2001, Pubmed
Behrens, The human taste receptor hTAS2R14 responds to a variety of different bitter compounds. 2004, Pubmed
Bufe, The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides. 2002, Pubmed
Béhé, Membrane currents in taste cells of the rat fungiform papilla. Evidence for two types of Ca currents and inhibition of K currents by saccharin. 1990, Pubmed
Caicedo, Role of the G-protein subunit alpha-gustducin in taste cell responses to bitter stimuli. 2003, Pubmed
Campos-Xavier, Chloride channel 7 (CLCN7) gene mutations in intermediate autosomal recessive osteopetrosis. 2003, Pubmed
Chandrashekar, T2Rs function as bitter taste receptors. 2000, Pubmed
Charlet-B, Loss of the muscle-specific chloride channel in type 1 myotonic dystrophy due to misregulated alternative splicing. 2002, Pubmed
Chaudhari, Molecular and physiological evidence for glutamate (umami) taste transduction via a G protein-coupled receptor. 1998, Pubmed
Chen, Exon 17 skipping in CLCN1 leads to recessive myotonia congenita. 2004, Pubmed , Xenbase
Chen, Characteristics of action potentials and their underlying outward currents in rat taste receptor cells. 1996, Pubmed
Chen, Electrophysiological actions of quinine on voltage-dependent currents in dissociated rat taste cells. 1997, Pubmed
Chu, A short CIC-2 mRNA transcript is produced by exon skipping. 1996, Pubmed
Chu, Alternative mRNA splice variants of the rat ClC-2 chloride channel gene are expressed in lung: genomic sequence and organization of ClC-2. 1997, Pubmed
Clapp, Immunocytochemical evidence for co-expression of Type III IP3 receptor with signaling components of bitter taste transduction. 2001, Pubmed
Cummings, Sweet taste transduction in hamster taste cells: evidence for the role of cyclic nucleotides. 1993, Pubmed
Dutzler, X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity. 2002, Pubmed
Eggermont, Alternative splicing of ClC-6 (a member of the CIC chloride-channel family) transcripts generates three truncated isoforms one of which, ClC-6c, is kidney-specific. 1997, Pubmed
Forino, Identification of a novel splice site mutation of CLCN5 gene and characterization of a new alternative 5' UTR end of ClC-5 mRNA in human renal tissue and leukocytes. 2004, Pubmed
Friedrich, Mutational analysis demonstrates that ClC-4 and ClC-5 directly mediate plasma membrane currents. 1999, Pubmed , Xenbase
Ghiaroni, Electrophysiological heterogeneity in a functional subset of mouse taste cells during postnatal development. 2003, Pubmed
Gilbertson, Hypoosmotic stimuli activate a chloride conductance in rat taste cells. 2002, Pubmed
Gilbertson, Proton currents through amiloride-sensitive Na channels in hamster taste cells. Role in acid transduction. 1992, Pubmed
Gilbertson, The molecular physiology of taste transduction. 2000, Pubmed
Goldin, Preparation of RNA for injection into Xenopus oocytes. 1992, Pubmed , Xenbase
Hara-Chikuma, Impaired acidification in early endosomes of ClC-5 deficient proximal tubule. 2005, Pubmed
Herness, Characterization of chloride currents and their noradrenergic modulation in rat taste receptor cells. 1999, Pubmed
Herness, Voltage-dependent sodium currents recorded from dissociated rat taste cells. 1995, Pubmed
Hofmann, TRPM5 is a voltage-modulated and Ca(2+)-activated monovalent selective cation channel. 2003, Pubmed
Hoon, Putative mammalian taste receptors: a class of taste-specific GPCRs with distinct topographic selectivity. 1999, Pubmed
Huang, Ggamma13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium. 1999, Pubmed
Kashiwayanagi, Voltage-dependent Ca2+ channel and Na+ channel in frog taste cells. 1983, Pubmed
Kawasaki, Identification of an acid-activated Cl(-) channel from human skeletal muscles. 1999, Pubmed
Kinnamon, Apical localization of K+ channels in taste cells provides the basis for sour taste transduction. 1988, Pubmed
Kitagawa, Molecular genetic identification of a candidate receptor gene for sweet taste. 2001, Pubmed
Li, Human receptors for sweet and umami taste. 2002, Pubmed
Lin, Acid-activated cation currents in rat vallate taste receptor cells. 2002, Pubmed
Lin, Epithelial Na+ channel subunits in rat taste cells: localization and regulation by aldosterone. 1999, Pubmed
Lindemann, Taste reception. 1996, Pubmed
Liu, Intracellular Ca2+ and the phospholipid PIP2 regulate the taste transduction ion channel TRPM5. 2003, Pubmed
Liu, Acidic stimuli activates two distinct pathways in taste receptor cells from rat fungiform papillae. 2001, Pubmed
Loewen, Isoform-specific exon skipping in a variant form of ClC-2. 2000, Pubmed
Ludewig, Two physically distinct pores in the dimeric ClC-0 chloride channel. 1996, Pubmed , Xenbase
Lyall, Decrease in rat taste receptor cell intracellular pH is the proximate stimulus in sour taste transduction. 2001, Pubmed
Lyall, Excitation and adaptation in the detection of hydrogen ions by taste receptor cells: a role for cAMP and Ca(2+). 2002, Pubmed
Matsunami, A family of candidate taste receptors in human and mouse. 2000, Pubmed
Max, Tas1r3, encoding a new candidate taste receptor, is allelic to the sweet responsiveness locus Sac. 2001, Pubmed
McLaughlin, Molecular cloning of G proteins and phosphodiesterases from rat taste cells. 1994, Pubmed
Mellman, Acidification of the endocytic and exocytic pathways. 1986, Pubmed
Middleton, Homodimeric architecture of a ClC-type chloride ion channel. 1996, Pubmed
Miyamoto, Sour transduction involves activation of NPPB-sensitive conductance in mouse taste cells. 1998, Pubmed
Mohammad-Panah, The chloride channel ClC-4 contributes to endosomal acidification and trafficking. 2003, Pubmed
Mohammad-Panah, The chloride channel ClC-4 co-localizes with cystic fibrosis transmembrane conductance regulator and may mediate chloride flux across the apical membrane of intestinal epithelia. 2002, Pubmed
Montmayeur, A candidate taste receptor gene near a sweet taste locus. 2001, Pubmed
Nelson, An amino-acid taste receptor. 2002, Pubmed
Nelson, Mammalian sweet taste receptors. 2001, Pubmed
Ogura, ClC-3B, a novel ClC-3 splicing variant that interacts with EBP50 and facilitates expression of CFTR-regulated ORCC. 2002, Pubmed
Prawitt, TRPM5 is a transient Ca2+-activated cation channel responding to rapid changes in [Ca2+]i. 2003, Pubmed
Pusch, Gating of the voltage-dependent chloride channel CIC-0 by the permeant anion. 1995, Pubmed , Xenbase
Pérez, A transient receptor potential channel expressed in taste receptor cells. 2002, Pubmed , Xenbase
Robinson, Identification of an N-terminal amino acid of the CLC-3 chloride channel critical in phosphorylation-dependent activation of a CaMKII-activated chloride current. 2004, Pubmed
Roper, Regenerative impulses in taste cells. 1983, Pubmed
Rugarli, Different chromosomal localization of the Clcn4 gene in Mus spretus and C57BL/6J mice. 1995, Pubmed
Ruiz, Behavioral evidence for a role of alpha-gustducin in glutamate taste. 2003, Pubmed
Rössler, G protein betagamma complexes in circumvallate taste cells involved in bitter transduction. 2000, Pubmed
Rössler, Identification of a phospholipase C beta subtype in rat taste cells. 1998, Pubmed
Sainz, Identification of a novel member of the T1R family of putative taste receptors. 2001, Pubmed
Schaeren-Wiemers, A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes. 1993, Pubmed
Shimada, Expression and canalicular localization of two isoforms of the ClC-3 chloride channel from rat hepatocytes. 2000, Pubmed
Stevens, Hyperpolarization-activated channels HCN1 and HCN4 mediate responses to sour stimuli. 2001, Pubmed
Stobrawa, Disruption of ClC-3, a chloride channel expressed on synaptic vesicles, leads to a loss of the hippocampus. 2001, Pubmed
Sun, Characterization of inwardly rectifying potassium currents from dissociated rat taste receptor cells. 1996, Pubmed
Ugawa, Receptor that leaves a sour taste in the mouth. 1998, Pubmed , Xenbase
Van der Kloot, A chloride channel blocker reduces acetylcholine uptake into synaptic vesicles at the frog neuromuscular junction. 2003, Pubmed
Vanoye, Functional characterization of recombinant human ClC-4 chloride channels in cultured mammalian cells. 2002, Pubmed
Varkevisser, Neural networks distinguish between taste qualities based on receptor cell population responses. 2001, Pubmed
Wladkowski, A basolateral chloride conductance in rat lingual epithelium. 1998, Pubmed
Wong, Transduction of bitter and sweet taste by gustducin. 1996, Pubmed
Yan, Bitter taste transduced by PLC-beta(2)-dependent rise in IP(3) and alpha-gustducin-dependent fall in cyclic nucleotides. 2001, Pubmed
Zhang, Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways. 2003, Pubmed