Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Constitutive secretion of exogenous neurotransmitter by nonneuronal cells: implications for neuronal secretion.
Chang S
,
Girod R
,
Morimoto T
,
O'Donoghue M
,
Popov S
.
???displayArticle.abstract???
Fibroblasts in cell culture were loaded with exogenous neurotransmitter acetylcholine (ACh). ACh secretion from loaded cells was detected by whole-cell patch clamp recordings from Xenopus myocytes manipulated into contact with ACh-loaded cells. Two different approaches were used for ACh loading. In the first approach, fibroblasts were incubated in the culture medium containing ACh. Recordings from myocytes revealed fast inward currents that resemble miniature endplate currents found at neuromuscular synapses. The currents observed in recordings from myocytes were due to exocytosis of ACh-containing vesicles. Although exogenous ACh penetrated through the plasma membrane of fibroblasts during incubation and was present in the cytoplasm at detectable levels, cytoplasmic ACh did not contribute to the quantal ACh secretion. In the second approach, exogenous ACh was loaded into the cytoplasm of fibroblasts by microinjection. Under these experimental conditions, fibroblasts also exhibited spontaneous quantal ACh secretion. Analysis of the exocytotic events in fibroblasts following two different protocols of ACh loading revealed that the vesicular compartments responsible for uptake of exogenous ACh are associated with the endocytic recycling pathway. Extrapolation of our results to neuronal cells suggest that in cholinergic neurons, in addition to genuine synaptic vesicles, ACh can be secreted by the vesicles participating in endosomal membrane recycling.
Alder,
Calcium-dependent transmitter secretion reconstituted in Xenopus oocytes: requirement for synaptophysin.
1992, Pubmed,
Xenbase
Alder,
Calcium-dependent transmitter secretion reconstituted in Xenopus oocytes: requirement for synaptophysin.
1992,
Pubmed
,
Xenbase
Alfonso,
The Caenorhabditis elegans unc-17 gene: a putative vesicular acetylcholine transporter.
1993,
Pubmed
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Augustine,
Calcium action in synaptic transmitter release.
1987,
Pubmed
Beckers,
Calcium and GTP: essential components in vesicular trafficking between the endoplasmic reticulum and Golgi apparatus.
1989,
Pubmed
Bekkers,
Origin of variability in quantal size in cultured hippocampal neurons and hippocampal slices.
1990,
Pubmed
Bennett,
The molecular machinery for secretion is conserved from yeast to neurons.
1993,
Pubmed
Bennett,
Ca2+ and the regulation of neurotransmitter secretion.
1997,
Pubmed
Buys,
Fusion of intracellular membrane pools with cell surfaces of macrophages stimulated by phorbol esters and calcium ionophores.
1984,
Pubmed
Calakos,
Synaptic vesicle biogenesis, docking, and fusion: a molecular description.
1996,
Pubmed
Cavalli,
Release of acetylcholine by Xenopus oocytes injected with mRNAs from cholinergic neurons.
1991,
Pubmed
,
Xenbase
Chavez,
A biosynthetic regulated secretory pathway in constitutive secretory cells.
1996,
Pubmed
Chow,
Release of acetylcholine from embryonic neurons upon contact with muscle cell.
1985,
Pubmed
,
Xenbase
Chow,
Delay in vesicle fusion revealed by electrochemical monitoring of single secretory events in adrenal chromaffin cells.
1992,
Pubmed
Coorssen,
Ca2+ triggers massive exocytosis in Chinese hamster ovary cells.
1996,
Pubmed
DEL CASTILLO,
Quantal components of the end-plate potential.
1954,
Pubmed
Dai,
Dynamics of synaptic vesicles in cultured spinal cord neurons in relationship to synaptogenesis.
1996,
Pubmed
,
Xenbase
Dan,
Evoked neuronal secretion of false transmitters.
1994,
Pubmed
,
Xenbase
Dan,
Quantal transmitter secretion from myocytes loaded with acetylcholine.
1992,
Pubmed
,
Xenbase
Evers,
Studies of nerve-muscle interactions in Xenopus cell culture: analysis of early synaptic currents.
1989,
Pubmed
,
Xenbase
Falk-Vairant,
Quantal acetylcholine release induced by mediatophore transfection.
1996,
Pubmed
Falk-Vairant,
Enhancement of quantal transmitter release and mediatophore expression by cyclic AMP in fibroblasts loaded with acetylcholine.
1996,
Pubmed
,
Xenbase
Girod,
Space and time characteristics of transmitter release at the nerve-electroplaque junction of Torpedo.
1993,
Pubmed
Girod,
Spontaneous quantal transmitter secretion from myocytes and fibroblasts: comparison with neuronal secretion.
1995,
Pubmed
,
Xenbase
Graessmann,
Microinjection of early SV40 DNA fragments and T antigen.
1980,
Pubmed
Griffiths,
A quantitative analysis of the endocytic pathway in baby hamster kidney cells.
1989,
Pubmed
Grynkiewicz,
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
1985,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Hanson,
Neurotransmitter release - four years of SNARE complexes.
1997,
Pubmed
Herreros,
Tetanus toxin inhibits spontaneous quantal release and cleaves VAMP/synaptobrevin.
1995,
Pubmed
Kidokoro,
Two types of miniature endplate potentials in Xenopus nerve-muscle cultures.
1984,
Pubmed
,
Xenbase
Kraszewski,
Synaptic vesicle dynamics in living cultured hippocampal neurons visualized with CY3-conjugated antibodies directed against the lumenal domain of synaptotagmin.
1995,
Pubmed
Kriebel,
Multimodal distribution of frog miniature endplate potentials in adult denervated and tadpole leg muscle.
1974,
Pubmed
Kriebel,
Hypertonic treatment reversibly increases the ratio of giant skew-miniature endplate potentials to bell-miniature endplate potentials.
1996,
Pubmed
Kriebel,
Spontaneous potentials and fine structure of identified frog denervated neuromuscular junctions.
1980,
Pubmed
Kriebel,
Effect of hypertonic saline on quantal size and synaptic vesicles in identified neuromuscular junction of the frog.
1987,
Pubmed
Mayor,
Sorting of membrane components from endosomes and subsequent recycling to the cell surface occurs by a bulk flow process.
1993,
Pubmed
Miyake,
Vesicle accumulation and exocytosis at sites of plasma membrane disruption.
1995,
Pubmed
Morimoto,
Calcium-dependent transmitter secretion from fibroblasts: modulation by synaptotagmin I.
1995,
Pubmed
,
Xenbase
Muniak,
Changes in MEPP and EPP amplitude distributions in the mouse diaphragm during synapse formation and degeneration.
1982,
Pubmed
Neher,
Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells.
1982,
Pubmed
Ninomiya,
Ca2+-dependent exocytotic pathways in Chinese hamster ovary fibroblasts revealed by a caged-Ca2+ compound.
1996,
Pubmed
Nusser,
Differences in synaptic GABA(A) receptor number underlie variation in GABA mini amplitude.
1997,
Pubmed
Parsons,
Acetylcholine transport, storage, and release.
1993,
Pubmed
Rodríguez,
Lysosomes behave as Ca2+-regulated exocytic vesicles in fibroblasts and epithelial cells.
1997,
Pubmed
Salter,
ATP-evoked increases in intracellular calcium in neurons and glia from the dorsal spinal cord.
1994,
Pubmed
Schiavo,
Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin.
1992,
Pubmed
Searl,
The effects of L-vesamicol, an inhibitor of vesicular acetylcholine uptake, on two populations of miniature endplate currents at the snake neuromuscular junction.
1990,
Pubmed
Song,
Expression of a putative vesicular acetylcholine transporter facilitates quantal transmitter packaging.
1997,
Pubmed
,
Xenbase
Steinhardt,
Cell membrane resealing by a vesicular mechanism similar to neurotransmitter release.
1994,
Pubmed
Söllner,
SNAP receptors implicated in vesicle targeting and fusion.
1993,
Pubmed
Südhof,
The synaptic vesicle cycle: a cascade of protein-protein interactions.
1995,
Pubmed
Terasaki,
Large plasma membrane disruptions are rapidly resealed by Ca2+-dependent vesicle-vesicle fusion events.
1997,
Pubmed
Vautrin,
Miniature endplate potentials induced by ammonium chloride, hypertonic shock, and botulinum toxin.
1992,
Pubmed
Vautrin,
Characteristics of slow-miniature endplate currents show a subunit composition.
1991,
Pubmed
Wightman,
Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells.
1991,
Pubmed
Williams,
How does a vesicle know it is full?
1997,
Pubmed
Xie,
Activity-dependent expression of NT-3 in muscle cells in culture: implications in the development of neuromuscular junctions.
1997,
Pubmed
,
Xenbase
Xie,
Initial events in the formation of neuromuscular synapse: rapid induction of acetylcholine release from embryonic neuron.
1986,
Pubmed
,
Xenbase
Zheng,
Turning of nerve growth cones induced by neurotransmitters.
1994,
Pubmed
,
Xenbase
