Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biochem J
1998 Oct 01;335 ( Pt 1):43-50. doi: 10.1042/bj3350043.
Show Gene links
Show Anatomy links
Characterization of p96h2bk: immunoreaction with an anti-Erk(extracellular-signal-regulated kinase) peptide antibody and activity in Xenopus oocytes and eggs.
Chen DH
,
Chen CT
,
Zhang Y
,
Liu MA
,
Campos-Gonzalez R
,
Pan BT
.
???displayArticle.abstract???
We have shown previously that oncogenic Ras induces cell cycle arrest in activated Xenopus egg extracts [Pan, Chen and Lin (1994) J. Biol. Chem. 269, 5968-5975]. The cell cycle arrest correlates with the stimulation of a protein kinase activity that phosphorylates histone H2b in vitro (designated p96(h2bk)) [Chen and Pan (1994) J. Biol. Chem. 269, 28034-28043]. We report here that p96(h2bk) is likely to be p96(ram), a protein of approx. 96 kDa that immunoreacts with a monoclonal antibody (Mk-1) raised against a synthetic peptide derived from a sequence highly conserved in Erk1/Erk2 (where Erk is extracellular-signal-regulated kinase). This is supported by two lines of evidence. First, activation/inactivation of p96(h2bk) correlates with upward/downward bandshifts of p96(ram) in polyacrylamide gels. Secondly, both p96(h2bk) and p96(ram) can be immunoprecipitated by antibody Mk-1. We also studied the activity of p96(h2bk)/p96(ram) in Xenopus oocytes and eggs. p96(h2bk)/p96(ram) was inactive in stage 6 oocytes, was active in unfertilized eggs, and became inactive again in eggs after fertilization. Since stage 6 oocytes are at G2-phase of the cell cycle, unfertilized eggs arrest at M-phase and eggs exit M-phase arrest after fertilization, the results thus indicate that p96(h2bk)/p96(ram) activity is cell cycle dependent. Moreover, microinjection of oncogenic Ras into fertilized eggs at the one-cell stage arrests the embryos at the two-cell stage, and this induced arrest is correlated with an inappropriate activation of p96(h2bk)/p96(ram). The data are consistent with the concept that inappropriate activation of p96(h2bk)/p96(ram) plays a role in the cell cycle arrest induced by oncogenic Ras.
Avruch,
Raf meets Ras: completing the framework of a signal transduction pathway.
1994, Pubmed
Avruch,
Raf meets Ras: completing the framework of a signal transduction pathway.
1994,
Pubmed
Barrett,
Ha-rasVal-12,Thr-59 activates S6 kinase and p34cdc2 kinase in Xenopus oocytes: evidence for c-mosxe-dependent and -independent pathways.
1990,
Pubmed
,
Xenbase
Bialojan,
Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatases. Specificity and kinetics.
1988,
Pubmed
Birchmeier,
ras proteins can induce meiosis in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Blenis,
Signal transduction via the MAP kinases: proceed at your own RSK.
1993,
Pubmed
Boulton,
ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.
1991,
Pubmed
Boulton,
Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies.
1991,
Pubmed
Burgering,
Epidermal growth factor induces phosphorylation of extracellular signal-regulated kinase 2 via multiple pathways.
1993,
Pubmed
Campos-González,
Temperature-dependent tyrosine phosphorylation of microtubule-associated protein kinase in epidermal growth factor-stimulated human fibroblasts.
1991,
Pubmed
Chen,
Oncogenic ras stimulates a 96-kDa histone H2b kinase activity in activated Xenopus egg extracts. Correlation with the suppression of p34cdc2 kinase.
1994,
Pubmed
,
Xenbase
Cobb,
How MAP kinases are regulated.
1995,
Pubmed
Cohen,
The structure and regulation of protein phosphatases.
1989,
Pubmed
Crews,
Extracellular signals and reversible protein phosphorylation: what to Mek of it all.
1993,
Pubmed
Daar,
The ras oncoprotein and M-phase activity.
1991,
Pubmed
,
Xenbase
Davis,
Transcriptional regulation by MAP kinases.
1995,
Pubmed
Fabian,
Requirement for Raf and MAP kinase function during the meiotic maturation of Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Fabian,
Critical tyrosine residues regulate the enzymatic and biological activity of Raf-1 kinase.
1993,
Pubmed
,
Xenbase
Feig,
Evidence for a Ras/Ral signaling cascade.
1996,
Pubmed
Fukuda,
Analysis of the Ras p21/mitogen-activated protein kinase signaling in vitro and in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Gotoh,
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
1991,
Pubmed
,
Xenbase
Gotoh,
Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade.
1995,
Pubmed
,
Xenbase
Haccard,
Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase.
1993,
Pubmed
,
Xenbase
Haccard,
Induction of Xenopus oocyte meiotic maturation by MAP kinase.
1995,
Pubmed
,
Xenbase
Hattori,
Activation of mitogen-activated protein kinase and its activator by ras in intact cells and in a cell-free system.
1992,
Pubmed
,
Xenbase
Hill,
Transcriptional regulation by extracellular signals: mechanisms and specificity.
1995,
Pubmed
Hirakawa,
Rescue of cells from ras oncogene-induced growth arrest by a second, complementing, oncogene.
1988,
Pubmed
Huang,
Biochemical and biological analysis of Mek1 phosphorylation site mutants.
1995,
Pubmed
,
Xenbase
Katz,
Signal transduction from multiple Ras effectors.
1997,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
MacNicol,
Regulation of Raf-1-dependent signaling during early Xenopus development.
1995,
Pubmed
,
Xenbase
Maller,
Regulation of amphibian oocyte maturation.
1985,
Pubmed
,
Xenbase
Marshall,
Specificity of receptor tyrosine kinase signaling: transient versus sustained extracellular signal-regulated kinase activation.
1995,
Pubmed
Marshall,
Ras effectors.
1996,
Pubmed
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Muslin,
Raf-1 protein kinase is important for progesterone-induced Xenopus oocyte maturation and acts downstream of mos.
1993,
Pubmed
,
Xenbase
Newport,
Regulation of the cell cycle during early Xenopus development.
1984,
Pubmed
,
Xenbase
Pan,
Role of phosphatidylinositide metabolism in ras-induced Xenopus oocyte maturation.
1990,
Pubmed
,
Xenbase
Pan,
Oncogenic Ras blocks cell cycle progression and inhibits p34cdc2 kinase in activated Xenopus egg extracts.
1994,
Pubmed
,
Xenbase
Pan,
The 96 kDa protein kinase activated by oncogenic Ras in Xenopus egg extracts is also activated by constitutively active Mek: activation requires serine/threonine phosphorylation.
1997,
Pubmed
,
Xenbase
Pomerance,
Stimulation of mitogen-activated protein kinase by oncogenic Ras p21 in Xenopus oocytes. Requirement for Ras p21-GTPase-activating protein interaction.
1992,
Pubmed
,
Xenbase
Posada,
Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
1991,
Pubmed
,
Xenbase
Ridley,
Ras-mediated cell cycle arrest is altered by nuclear oncogenes to induce Schwann cell transformation.
1988,
Pubmed
Rodriguez-Viciana,
Phosphatidylinositol-3-OH kinase as a direct target of Ras.
1994,
Pubmed
Rodriguez-Viciana,
Role of phosphoinositide 3-OH kinase in cell transformation and control of the actin cytoskeleton by Ras.
1997,
Pubmed
Shibuya,
Oncogenic ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Sturgill,
Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
1988,
Pubmed
,
Xenbase
Wasserman,
The maturation response of stage IV, V, and VI Xenopus oocytes to progesterone stimulation in vitro.
1984,
Pubmed
,
Xenbase
Zhou,
Components of a new human protein kinase signal transduction pathway.
1995,
Pubmed
Zhu,
Cloning and characterization of p97MAPK, a novel human homolog of rat ERK-3.
1994,
Pubmed
