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J Bacteriol
1998 Nov 01;18021:5765-8. doi: 10.1128/JB.180.21.5765-5768.1998.
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Methylation of FrzCD defines a discrete step in the developmental program of Myxococcus xanthus.
Geng Y
,
Yang Z
,
Downard J
,
Zusman D
,
Shi W
.
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Myxococcus xanthus is a gram-negative soil bacterium which undergoes fruiting body formation during starvation. The frz signal transduction system has been found to play an important role in this process. FrzCD, a methyl-accepting taxis protein homologue, shows modulated methylation during cellular aggregation, which is thought to be part of an adaptation response to an aggregation signal. In this study, we assayed FrzCD methylation in many known and newly isolated mutants defective in fruiting body formation to determine a possible relationship between the methylation response and fruiting morphology. The results of our analysis indicated that the developmental mutants could be divided into two groups based on their ability to show normal FrzCD methylation during development. Many mutants blocked early in development, i.e., nonaggregating or abnormally aggregating mutants, showed poor FrzCD methylation. The well-characterized asg, bsg, csg, and esg mutants were found to be of this type. The defects in FrzCD methylation of these signaling mutants could be partially rescued by extracellular complementation with wild-type cells or addition of chemicals which restore their fruiting body formation. Mutants blocked in late development, i.e., translucent mounds, showed normal FrzCD methylation. Surprisingly, some mutants blocked in early development also exhibited a normal level of FrzCD methylation. The characterized mutants in this group were found to be defective in social motility. This indicates that FrzCD methylation defines a discrete step in the development of M. xanthus and that social motility mutants are not blocked in these early developmental steps.
Campos,
Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus.
1978, Pubmed
Campos,
Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus.
1978,
Pubmed
Downard,
Branched-chain fatty acids: the case for a novel form of cell-cell signalling during Myxococcus xanthus development.
1995,
Pubmed
Downard,
Identification of esg, a genetic locus involved in cell-cell signaling during Myxococcus xanthus development.
1993,
Pubmed
Gill,
Control of developmental gene expression by cell-to-cell interactions in Myxococcus xanthus.
1986,
Pubmed
Hagen,
Synergism between morphogenetic mutants of Myxococcus xanthus.
1978,
Pubmed
Kaiser,
How and why bacteria talk to each other.
1993,
Pubmed
Kuner,
Introduction of transposon Tn5 into Myxococcus for analysis of developmental and other nonselectable mutants.
1981,
Pubmed
Kuspa,
Genes required for developmental signalling in Myxococcus xanthus: three asg loci.
1989,
Pubmed
McBride,
Methylation of FrzCD, a methyl-accepting taxis protein of Myxococcus xanthus, is correlated with factors affecting cell behavior.
1992,
Pubmed
McBride,
FrzCD, a methyl-accepting taxis protein from Myxococcus xanthus, shows modulated methylation during fruiting body formation.
1993,
Pubmed
McBride,
"Frizzy" aggregation genes of the gliding bacterium Myxococcus xanthus show sequence similarities to the chemotaxis genes of enteric bacteria.
1989,
Pubmed
McCleary,
FrzE of Myxococcus xanthus is homologous to both CheA and CheY of Salmonella typhimurium.
1990,
Pubmed
McCleary,
Developmental sensory transduction in Myxococcus xanthus involves methylation and demethylation of FrzCD.
1990,
Pubmed
O'Connor,
Genetic analysis of Myxococcus xanthus and isolation of gene replacements after transduction under conditions of limited homology.
1986,
Pubmed
Shi,
Isolation and phenotypic characterization of Myxococcus xanthus mutants which are defective in sensing negative stimuli.
1994,
Pubmed
Shi,
Cell density regulates cellular reversal frequency in Myxococcus xanthus.
1996,
Pubmed
Shi,
The two motility systems of Myxococcus xanthus show different selective advantages on various surfaces.
1993,
Pubmed
Shi,
Chemotaxis plays a role in the social behaviour of Myxococcus xanthus.
1993,
Pubmed
Shimkets,
Developmental cell interactions in Myxococcus xanthus and the spoC locus.
1983,
Pubmed
Søgaard-Andersen,
C factor, a cell-surface-associated intercellular signaling protein, stimulates the cytoplasmic Frz signal transduction system in Myxococcus xanthus.
1996,
Pubmed
Toal,
The esg locus of Myxococcus xanthus encodes the E1 alpha and E1 beta subunits of a branched-chain keto acid dehydrogenase.
1995,
Pubmed
Yang,
A new set of chemotaxis homologues is essential for Myxococcus xanthus social motility.
1998,
Pubmed
Yang,
A DnaK homolog in Myxococcus xanthus is involved in social motility and fruiting body formation.
1998,
Pubmed
Zusman,
"Frizzy" mutants: a new class of aggregation-defective developmental mutants of Myxococcus xanthus.
1982,
Pubmed