Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1998 Nov 10;9523:13442-7. doi: 10.1073/pnas.95.23.13442.
Show Gene links
Show Anatomy links
Retinoic acid X receptor in the diploblast, Tripedalia cystophora.
Kostrouch Z
,
Kostrouchova M
,
Love W
,
Jannini E
,
Piatigorsky J
,
Rall JE
.
???displayArticle.abstract???
Nuclear hormone receptors comprise a characteristic family of transcription factors found in vertebrates, insects and nematodes. Here we show by cDNA and gene cloning that a Cnidarian, Tripedalia cystophora, possesses a retinoid receptor (jRXR) with remarkable homology to vertebrate retinoic acid X receptors (RXRs). Like vertebrate RXRs, jRXR binds 9-cis retinoic acid (Kd = 4 x 10(-10) M) and binds to the DNA sequence, PuGGTCA as a monomer in vitro. jRXR also heterodimerizes with Xenopus TR beta on a thyroid responsive element of a direct repeat separated by 4 bp. A jRXR binding half-site capable of interacting with (His6)jRXR fusion protein was identified in the promoters of three T. cystophora crystallin genes that are expressed highly in the eyelens of this jellyfish. Because crystallin gene expression is regulated by retionoid signaling in vertebrates, the jellyfish crystallin genes are candidate in vivo targets for jRXR. Finally, an antibody prepared against (His6)jRXR showed that full-length jRXR is expressed at all developmental stages of T. cystophora except the ephydra, where a smaller form replaces is. These data show that Cnidaria, a diploblastic phylum ancestral to the triploblastic invertebrate and subsequent vertebrate lineages, already have an RXR suggesting that RXR is an early component of the regulatory mechanisms of metazoa.
Blumberg,
BXR, an embryonic orphan nuclear receptor activated by a novel class of endogenous benzoate metabolites.
1998, Pubmed,
Xenbase
Blumberg,
BXR, an embryonic orphan nuclear receptor activated by a novel class of endogenous benzoate metabolites.
1998,
Pubmed
,
Xenbase
Clagett-Dame,
Generating and characterizing retinoid receptors from Escherichia coli and insect cell expression systems.
1997,
Pubmed
Cvekl,
Lens development and crystallin gene expression: many roles for Pax-6.
1996,
Pubmed
Escriva,
Ligand binding was acquired during evolution of nuclear receptors.
1997,
Pubmed
Forman,
Identification of a nuclear receptor that is activated by farnesol metabolites.
1995,
Pubmed
Forman,
The orphan nuclear receptor LXRalpha is positively and negatively regulated by distinct products of mevalonate metabolism.
1997,
Pubmed
Gopal-Srivastava,
Involvement of retinoic acid/retinoid receptors in the regulation of murine alphaB-crystallin/small heat shock protein gene expression in the lens.
1998,
Pubmed
Jones,
Ultraspiracle: an invertebrate nuclear receptor for juvenile hormones.
1997,
Pubmed
Kastner,
Genetic analysis of RXR alpha developmental function: convergence of RXR and RAR signaling pathways in heart and eye morphogenesis.
1994,
Pubmed
Kliewer,
Retinoid X receptor interacts with nuclear receptors in retinoic acid, thyroid hormone and vitamin D3 signalling.
1992,
Pubmed
Kostrouch,
Steroid/thyroid hormone receptor genes in Caenorhabditis elegans.
1995,
Pubmed
Lala,
Activation of the orphan nuclear receptor steroidogenic factor 1 by oxysterols.
1997,
Pubmed
Laudet,
Evolution of the nuclear receptor superfamily: early diversification from an ancestral orphan receptor.
1997,
Pubmed
Laudet,
Evolution of the nuclear receptor gene superfamily.
1992,
Pubmed
Levin,
9-cis retinoic acid stereoisomer binds and activates the nuclear receptor RXR alpha.
1992,
Pubmed
Li,
Lens-preferred activity of chicken delta 1- and delta 2-crystallin enhancers in transgenic mice and evidence for retinoic acid-responsive regulation of the delta 1-crystallin gene.
1997,
Pubmed
Liu,
The mouse retinoid-X receptor-gamma gene: genomic organization and evidence for functional isoforms.
1993,
Pubmed
Mangelsdorf,
The RXR heterodimers and orphan receptors.
1995,
Pubmed
Mangelsdorf,
The nuclear receptor superfamily: the second decade.
1995,
Pubmed
Martínez,
Cnidarian homeoboxes and the zootype.
1998,
Pubmed
Nagata,
The mouse Rxrb gene encoding RXR beta: genomic organization and two mRNA isoforms generated by alternative splicing of transcripts initiated from CpG island promoters.
1994,
Pubmed
Oro,
Relationship between the product of the Drosophila ultraspiracle locus and the vertebrate retinoid X receptor.
1990,
Pubmed
Patek,
Age-related changes in the response of chick lens cells during long-term culture to insulin, cyclic AMP, retinoic acid and a bovine retinal extract.
1990,
Pubmed
Piatigorsky,
The cellular eye lens and crystallins of cubomedusan jellyfish.
1989,
Pubmed
Piatigorsky,
J1-crystallins of the cubomedusan jellyfish lens constitute a novel family encoded in at least three intronless genes.
1993,
Pubmed
Sun,
Evolution of paired domains: isolation and sequencing of jellyfish and hydra Pax genes related to Pax-5 and Pax-6.
1997,
Pubmed
Tini,
An everted repeat mediates retinoic acid induction of the gamma F-crystallin gene: evidence of a direct role for retinoids in lens development.
1993,
Pubmed
Tini,
Functional interactions between retinoic acid receptor-related orphan nuclear receptor (ROR alpha) and the retinoic acid receptors in the regulation of the gamma F-crystallin promoter.
1995,
Pubmed
Tini,
Heterodimeric interaction of the retinoic acid and thyroid hormone receptors in transcriptional regulation on the gamma F-crystallin everted retinoic acid response element.
1994,
Pubmed
Valentine,
Cleavage patterns and the topology of the metazoan tree of life.
1997,
Pubmed
Werner,
Life cycle of Tripedalia cystophora Conant (Cubomedusae).
1971,
Pubmed
Wilson,
Identification of the DNA binding site for NGFI-B by genetic selection in yeast.
1991,
Pubmed