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XB-ART-13569
FEBS Lett 1999 Jan 15;4422-3:173-7.
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Evidence for calmodulin inter-domain compaction in solution induced by W-7 binding.

Osawa M , Kuwamoto S , Izumi Y , Yap KL , Ikura M , Shibanuma T , Yokokura H , Hidaka H , Matsushima N .


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Small-angle X-ray scattering and nuclear magnetic resonance were used to investigate the structural change of calcium-bound calmodulin (Ca2+/CaM) in solution upon binding to its antagonist, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7). The radius of gyration was 17.4+/-0.3 A for Ca2+/CaM-W-7 with a molar ratio of 1:5 and 20.3+/-0.7 A for Ca2+/CaM. Comparison of the radius of gyration and the pair distance distribution function of the Ca2+/CaM-W-7 complex with those of other complexes indicates that binding of two W-7 molecules induces a globular shape for Ca2+/CaM, probably caused by an inter-domain compaction. The results suggest a tendency for Ca2+/CaM to form a globular structure in solution, which is inducible by a small compound like W-7.

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