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Histochem J
1998 Mar 01;303:189-96. doi: 10.1023/a:1003247403685.
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Fluorescence microscopy of calcium and synaptic vesicle dynamics during synapse formation in tissue culture.
Dai Z
,
Peng HB
.
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The signal transduction process involved in the development of the nerve terminal is an intriguing question in developmental neurobiology. During the formation of the neuromuscular junction, presynaptic development is induced by growth cone's contact with the target muscle cell. Fluorescence microscopy with specific markers has made it possible to follow signalling events during this process. By using fluorescent calcium indicators, such as fura-2 and fluo-3, we found that a rise in intracellular calcium is elicited in the growth cone upon its contact with a target, and this calcium signal can also be elicited by local application of basic fibroblast growth factor. To monitor the clustering of synaptic vesicles in response to target contact, the fluorescent vesicular probe FMl-43 was used. With this probe, we observed that packets of synaptic vesicle are already present along the length of naïve neurite, which has not encountered its synaptic target. The activity-dependent loading of FMl-43 indicates that these packets can undergo exocytosis and endocytosis upon depolarization. Time-lapse recording showed that these packets are quite mobile. Upon target contact, synaptic vesicles become clustered and immobilized at the contact site. The methodology and instrumentation used in these studies are described in this article.
Betz,
Activity-dependent fluorescent staining and destaining of living vertebrate motor nerve terminals.
1992, Pubmed
Betz,
Activity-dependent fluorescent staining and destaining of living vertebrate motor nerve terminals.
1992,
Pubmed
Betz,
Optical analysis of synaptic vesicle recycling at the frog neuromuscular junction.
1992,
Pubmed
Betz,
Synaptic transmission. Kinetics of synaptic-vesicle recycling.
1995,
Pubmed
Dai,
Presynaptic differentiation induced in cultured neurons by local application of basic fibroblast growth factor.
1995,
Pubmed
,
Xenbase
Dai,
Elevation in presynaptic Ca2+ level accompanying initial nerve-muscle contact in tissue culture.
1993,
Pubmed
,
Xenbase
Dai,
Dynamics of synaptic vesicles in cultured spinal cord neurons in relationship to synaptogenesis.
1996,
Pubmed
,
Xenbase
Dunaevsky,
Long-term maintenance of presynaptic function in the absence of target muscle fibers.
1995,
Pubmed
Evers,
Studies of nerve-muscle interactions in Xenopus cell culture: analysis of early synaptic currents.
1989,
Pubmed
,
Xenbase
Grynkiewicz,
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
1985,
Pubmed
Hall,
Synaptic structure and development: the neuromuscular junction.
1993,
Pubmed
Henkel,
FM1-43 dye ultrastructural localization in and release from frog motor nerve terminals.
1996,
Pubmed
Kidokoro,
Initial synaptic transmission at the growth cone in Xenopus nerve-muscle cultures.
1982,
Pubmed
,
Xenbase
Minta,
Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores.
1989,
Pubmed
Nakajima,
The development of functional neuromuscular junctions in vitro: an ultrastructural and physiological study.
1980,
Pubmed
Peng,
Tissue culture of Xenopus neurons and muscle cells as a model for studying synaptic induction.
1991,
Pubmed
,
Xenbase
Ryan,
The kinetics of synaptic vesicle recycling measured at single presynaptic boutons.
1993,
Pubmed
Takahashi,
Structure and physiology of developing neuromuscular synapses in culture.
1987,
Pubmed
,
Xenbase
Xie,
Initial events in the formation of neuromuscular synapse: rapid induction of acetylcholine release from embryonic neuron.
1986,
Pubmed
,
Xenbase
Zoran,
Neuron-muscle contact changes presynaptic resting calcium set-point.
1993,
Pubmed