Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1999 Jun 01;196:4039-46. doi: 10.1128/MCB.19.6.4039.
Show Gene links
Show Anatomy links
The accessory subunit of Xenopus laevis mitochondrial DNA polymerase gamma increases processivity of the catalytic subunit of human DNA polymerase gamma and is related to class II aminoacyl-tRNA synthetases.
Carrodeguas JA
,
Kobayashi R
,
Lim SE
,
Copeland WC
,
Bogenhagen DF
.
???displayArticle.abstract???
Peptide sequences obtained from the accessory subunit of Xenopus laevis mitochondrial DNA (mtDNA) polymerase gamma (pol gamma) were used to clone the cDNA encoding this protein. Amino-terminal sequencing of the mitochondrial protein indicated the presence of a 44-amino-acid mitochondrial targeting sequence, leaving a predicted mature protein with 419 amino acids and a molecular mass of 47.3 kDa. This protein is associated with the larger, catalytic subunit in preparations of active mtDNA polymerase. The small subunit exhibits homology to its human, mouse, and Drosophila counterparts. Interestingly, significant homology to glycyl-tRNA synthetases from prokaryotic organisms reveals a likely evolutionary relationship. Since attempts to produce an enzymatically active recombinant catalytic subunit of Xenopus DNA pol gamma have not been successful, we tested the effects of adding the small subunit of the Xenopus enzyme to the catalytic subunit of human DNA pol gamma purified from baculovirus-infected insect cells. These experiments provide the first functional evidence that the small subunit of DNA pol gamma stimulates processive DNA synthesis by the human catalytic subunit under physiological salt conditions.
Bedford,
The thioredoxin binding domain of bacteriophage T7 DNA polymerase confers processivity on Escherichia coli DNA polymerase I.
1997, Pubmed
Bedford,
The thioredoxin binding domain of bacteriophage T7 DNA polymerase confers processivity on Escherichia coli DNA polymerase I.
1997,
Pubmed
Bochkarev,
Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA.
1997,
Pubmed
Cermakian,
Sequences homologous to yeast mitochondrial and bacteriophage T3 and T7 RNA polymerases are widespread throughout the eukaryotic lineage.
1996,
Pubmed
Curth,
Single-stranded-DNA-binding proteins from human mitochondria and Escherichia coli have analogous physicochemical properties.
1994,
Pubmed
Don,
'Touchdown' PCR to circumvent spurious priming during gene amplification.
1991,
Pubmed
Foury,
Cloning and sequencing of the nuclear gene MIP1 encoding the catalytic subunit of the yeast mitochondrial DNA polymerase.
1989,
Pubmed
Fraser,
Amino acids are not all initially attached to the same position on transfer RNA molecules.
1975,
Pubmed
Freist,
Glycyl-tRNA synthetase.
1996,
Pubmed
Ghrir,
Primary structure of the two variants of Xenopus laevis mtSSB, a mitochondrial DNA binding protein.
1991,
Pubmed
,
Xenbase
Graves,
Expression, purification, and initial kinetic characterization of the large subunit of the human mitochondrial DNA polymerase.
1998,
Pubmed
Gray,
Purification and identification of subunit structure of the human mitochondrial DNA polymerase.
1992,
Pubmed
Guan,
Cytoplasmic tyrosyl-tRNA synthetase rescues the defect in mitochondrial genome maintenance caused by the nuclear mutation mgm104-1 in the yeast Saccharomyces cerevisiae.
1997,
Pubmed
Insdorf,
DNA polymerase gamma from Xenopus laevis. I. The identification of a high molecular weight catalytic subunit by a novel DNA polymerase photolabeling procedure.
1989,
Pubmed
,
Xenbase
Lecrenier,
Mitochondrial DNA polymerases from yeast to man: a new family of polymerases.
1997,
Pubmed
,
Xenbase
Lewis,
Catalytic subunit of mitochondrial DNA polymerase from Drosophila embryos. Cloning, bacterial overexpression, and biochemical characterization.
1996,
Pubmed
Logan,
Crystal structure of glycyl-tRNA synthetase from Thermus thermophilus.
1995,
Pubmed
Longley,
Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease activity and dideoxynucleotide sensitivity.
1998,
Pubmed
Mahoungou,
The amino-terminal sequence of the Xenopus laevis mitochondrial SSB is homologous to that of the Escherichia coli protein.
1988,
Pubmed
,
Xenbase
Masters,
Yeast mitochondrial RNA polymerase is homologous to those encoded by bacteriophages T3 and T7.
1987,
Pubmed
Mazauric,
Glycyl-tRNA synthetase from Thermus thermophilus--wide structural divergence with other prokaryotic glycyl-tRNA synthetases and functional inter-relation with prokaryotic and eukaryotic glycylation systems.
1998,
Pubmed
Mikhailov,
Effects of Xenopus laevis mitochondrial single-stranded DNA-binding protein on primer-template binding and 3'-->5' exonuclease activity of DNA polymerase gamma.
1996,
Pubmed
,
Xenbase
Olson,
Subunit structure of mitochondrial DNA polymerase from Drosophila embryos. Physical and immunological studies.
1995,
Pubmed
Parisi,
Similarity of human mitochondrial transcription factor 1 to high mobility group proteins.
1991,
Pubmed
Ropp,
Characterization of a new DNA polymerase from Schizosaccharomyces pombe: a probable homologue of the Saccharomyces cerevisiae DNA polymerase gamma.
1995,
Pubmed
Ropp,
Cloning and characterization of the human mitochondrial DNA polymerase, DNA polymerase gamma.
1996,
Pubmed
Sprinzl,
Site of aminoacylation of tRNAs from Escherichia coli with respect to the 2'- or 3'-hydroxyl group of the terminal adenosine.
1975,
Pubmed
Tabor,
Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7.
1987,
Pubmed
Van Dyck,
A single-stranded DNA binding protein required for mitochondrial DNA replication in S. cerevisiae is homologous to E. coli SSB.
1992,
Pubmed
,
Xenbase
Wang,
Cellular adherence elicits ligand-independent activation of the Met cell-surface receptor.
1996,
Pubmed
Wang,
Accessory subunit of mitochondrial DNA polymerase from Drosophila embryos. Cloning, molecular analysis, and association in the native enzyme.
1997,
Pubmed
Ye,
The gamma subfamily of DNA polymerases: cloning of a developmentally regulated cDNA encoding Xenopus laevis mitochondrial DNA polymerase gamma.
1996,
Pubmed
,
Xenbase