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Genes Dev
1999 May 15;1310:1289-96. doi: 10.1101/gad.13.10.1289.
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Assembly of functionally active Drosophila origin recognition complex from recombinant proteins.
Chesnokov I
,
Gossen M
,
Remus D
,
Botchan M
.
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In eukaryotes the sites for the initiation of chromosomal DNA replication are believed to be determined in part by the binding of a heteromeric origin recognition complex (ORC) to DNA. We have cloned the genes encoding the subunits of the Drosophila ORC. Each of the genes is unique and can be mapped to discrete chromosomal locations implying that the pattern and developmental regulation of origin usage in Drosophila is not regulated solely by a large family of different ORC proteins. The six-subunit ORC can be reconstituted with recombinant proteins into a complex that restores DNA replication in ORC-depleted Drosophila or Xenopus egg extracts.
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10346817
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Aladjem,
Genetic dissection of a mammalian replicator in the human beta-globin locus.
1998, Pubmed
Aladjem,
Genetic dissection of a mammalian replicator in the human beta-globin locus.
1998,
Pubmed
Aparicio,
Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
1997,
Pubmed
Bell,
ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex.
1992,
Pubmed
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Blow,
A role for the nuclear envelope in controlling DNA replication within the cell cycle.
1988,
Pubmed
,
Xenbase
Brown,
Functional cDNA libraries from Drosophila embryos.
1988,
Pubmed
Carpenter,
Role for a Xenopus Orc2-related protein in controlling DNA replication.
1996,
Pubmed
,
Xenbase
Carpenter,
Identification of a novel 81-kDa component of the Xenopus origin recognition complex.
1998,
Pubmed
,
Xenbase
Crevel,
DNA replication in cell-free extracts from Drosophila melanogaster.
1991,
Pubmed
,
Xenbase
Diffley,
Two steps in the assembly of complexes at yeast replication origins in vivo.
1994,
Pubmed
,
Xenbase
Diffley,
Once and only once upon a time: specifying and regulating origins of DNA replication in eukaryotic cells.
1996,
Pubmed
Dutta,
Initiation of DNA replication in eukaryotic cells.
1997,
Pubmed
Gavin,
Conserved initiator proteins in eukaryotes.
1995,
Pubmed
Gilbert,
Replication origins in yeast versus metazoa: separation of the haves and the have nots.
1998,
Pubmed
,
Xenbase
Gossen,
A Drosophila homolog of the yeast origin recognition complex.
1995,
Pubmed
Grallert,
The ORC1 homolog orp1 in fission yeast plays a key role in regulating onset of S phase.
1996,
Pubmed
Heck,
Multiple replication origins are used during Drosophila chorion gene amplification.
1990,
Pubmed
Hua,
Identification of a preinitiation step in DNA replication that is independent of origin recognition complex and cdc6, but dependent on cdk2.
1998,
Pubmed
,
Xenbase
Ishiai,
Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22.
1997,
Pubmed
Klemm,
Coordinate binding of ATP and origin DNA regulates the ATPase activity of the origin recognition complex.
1997,
Pubmed
Landis,
The k43 gene, required for chorion gene amplification and diploid cell chromosome replication, encodes the Drosophila homolog of yeast origin recognition complex subunit 2.
1997,
Pubmed
Leatherwood,
Interaction of Cdc2 and Cdc18 with a fission yeast ORC2-like protein.
1996,
Pubmed
Lee,
Architecture of the yeast origin recognition complex bound to origins of DNA replication.
1997,
Pubmed
Muzi-Falconi,
Orp1, a member of the Cdc18/Cdc6 family of S-phase regulators, is homologous to a component of the origin recognition complex.
1995,
Pubmed
Newlon,
Putting it all together: building a prereplicative complex.
1997,
Pubmed
,
Xenbase
Ohtani,
Expression of the HsOrc1 gene, a human ORC1 homolog, is regulated by cell proliferation via the E2F transcription factor.
1996,
Pubmed
Pak,
Association of the origin recognition complex with heterochromatin and HP1 in higher eukaryotes.
1997,
Pubmed
,
Xenbase
Quintana,
Identification of HsORC4, a member of the human origin of replication recognition complex.
1997,
Pubmed
Quintana,
ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases.
1998,
Pubmed
Romanowski,
The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin.
1996,
Pubmed
,
Xenbase
Rowles,
Interaction between the origin recognition complex and the replication licensing system in Xenopus.
1996,
Pubmed
,
Xenbase
Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Stillman,
Cell cycle control of DNA replication.
1996,
Pubmed
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Tugal,
The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p.
1998,
Pubmed
,
Xenbase
Zou,
Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin.
1998,
Pubmed
