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Proc Biol Sci
1999 Jun 22;2661425:1207-13. doi: 10.1098/rspb.1999.0764.
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Low expression of the ClC-2 chloride channel during postnatal development: a mechanism for the paradoxical depolarizing action of GABA and glycine in the hippocampus.
Mladini M
,
Becchetti A
,
Didelon F
,
Bradbury A
,
Cherubini E
.
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In early postnatal development, during the period of synapse formation, gamma-aminobutyric acid (GABA) and glycine, the main inhibitory transmitters in the adult brain, paradoxically excite and depolarize neuronal membranes by an outward flux of chloride. The mechanisms of chloride homeostasis are not fully understood. It is known that in adult neurons intracellular chloride accumulation is prevented by a particular type of chloride channel, the ClC-2. This channel strongly rectifies in the inward direction at potentials negative to ECl thus ensuring chloride efflux. We have tested the hypothesis that in the developing hippocampus, a differential expression or regulation of ClC-2 channels may contribute to the depolarizing action of GABA and glycine. We have cloned a truncated form of ClC-2 (ClC-2nh) from the neonatal hippocampus which lacks the 157 bp corresponding to exon 2. In situ hybridization experiments show that ClC-2nh is the predominant form of ClC-2 mRNA in the neonatal brain. ClC-2nh mRNA is unable to encode a full-length protein due to a frameshift, consequently it does not induce any currents upon injection into Xenopus oocytes. Low expression of the full-length ClC-2 channel, could alter chloride homeostasis, lead to accumulation of [Cl-]i and thereby contribute to the depolarizing action of GABA and glycine during early development.
Ben-Ari,
Giant synaptic potentials in immature rat CA3 hippocampal neurones.
1989, Pubmed
Ben-Ari,
Giant synaptic potentials in immature rat CA3 hippocampal neurones.
1989,
Pubmed
Cherubini,
GABA: an excitatory transmitter in early postnatal life.
1991,
Pubmed
Chu,
Alternative mRNA splice variants of the rat ClC-2 chloride channel gene are expressed in lung: genomic sequence and organization of ClC-2.
1997,
Pubmed
Daniels,
Detection of a novel splice variant of the hypoxanthine-guanine phosphoribosyl transferase gene in human oocytes and preimplantation embryos: implications for a RT-PCR-based preimplantation diagnosis of Lesch-Nyhan syndrome.
1998,
Pubmed
Gegelashvili,
High affinity glutamate transporters: regulation of expression and activity.
1997,
Pubmed
Goodman,
Developmental mechanisms that generate precise patterns of neuronal connectivity.
1993,
Pubmed
Gründer,
Regions involved in the opening of CIC-2 chloride channel by voltage and cell volume.
,
Pubmed
,
Xenbase
Ito,
Strychnine-sensitive glycine responses of neonatal rat hippocampal neurones.
1991,
Pubmed
Jentsch,
Chloride channels: a molecular perspective.
1996,
Pubmed
,
Xenbase
Jentsch,
Chloride channels: an emerging molecular picture.
1997,
Pubmed
Jin,
Developmentally regulated muscle type-specific alternative splicing of the COOH-terminal variable region of fast skeletal muscle troponin T and an aberrant splicing pathway to encode a mutant COOH-terminus.
1998,
Pubmed
Jordt,
Molecular dissection of gating in the ClC-2 chloride channel.
1997,
Pubmed
,
Xenbase
Kaila,
The role of bicarbonate in GABAA receptor-mediated IPSPs of rat neocortical neurones.
1993,
Pubmed
Kaneda,
Whole-cell and single-channel currents activated by GABA and glycine in granule cells of the rat cerebellum.
1995,
Pubmed
Misgeld,
The role of chloride transport in postsynaptic inhibition of hippocampal neurons.
1986,
Pubmed
Ohno,
Targeted disruption of the CD3 eta locus causes high lethality in mice: modulation of Oct-1 transcription on the opposite strand.
1994,
Pubmed
Owens,
Excitatory GABA responses in embryonic and neonatal cortical slices demonstrated by gramicidin perforated-patch recordings and calcium imaging.
1996,
Pubmed
Payne,
Functional characterization of the neuronal-specific K-Cl cotransporter: implications for [K+]o regulation.
1997,
Pubmed
Reichling,
Mechanisms of GABA and glycine depolarization-induced calcium transients in rat dorsal horn neurons.
1994,
Pubmed
Rivera,
The K+/Cl- co-transporter KCC2 renders GABA hyperpolarizing during neuronal maturation.
1999,
Pubmed
Smith,
Differential expression of an inwardly rectifying chloride conductance in rat brain neurons: a potential mechanism for cell-specific modulation of postsynaptic inhibition.
1995,
Pubmed
Staley,
The role of an inwardly rectifying chloride conductance in postsynaptic inhibition.
1994,
Pubmed
Staley,
Ionic mechanisms of neuronal excitation by inhibitory GABAA receptors.
1995,
Pubmed
Staley,
Alteration of GABAA receptor function following gene transfer of the CLC-2 chloride channel.
1996,
Pubmed
Thiemann,
A chloride channel widely expressed in epithelial and non-epithelial cells.
1992,
Pubmed
,
Xenbase
Wu,
Early development of glycine- and GABA-mediated synapses in rat spinal cord.
1992,
Pubmed
