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Influence of inorganic phosphate and pH on sarcoplasmic reticular ATPase in skinned muscle fibres of Xenopus laevis.
Stienen GJ
,
Papp Z
,
Zaremba R
.
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1. The influence of 30 mM inorganic phosphate (Pi) and pH (6.2-7.4) on the rate of ATP utilization was determined in mechanically skinned bundles of myofibrils from the iliofibularismuscle of Xenopus laevis at approximately 5 C. 2. BDM (2,3-butanedione monoxime; 10 mM) depressed isometric force production and actomyosin (AM) ATPase activity equally. Therefore sarcoplasmic reticular (SR) ATPase activity could be determined by extrapolation of the total ATPase activity to zero force. 3. The SR ATPase activity without added Pi at pH 7.1 was 42 +/- 2 % of the total ATPase activity. Addition of 30 mM Pi reduced SR ATPase activity slightly, by 9 +/- 5 %, and depressed force by 62 +/- 2 % and AM ATPase activity by 21 +/- 6 %. 4. At pH 6.2, force, SR ATPase activity and AM ATPase activity were reduced by 21 +/- 5, 61 +/- 5 and 10 +/- 4 % of their respective values at pH 7.1. 5. The SR ATPase activity at 30 mM Pi and pH 6.2 was reduced markedly to 20 +/- 6 % of the value under control conditions, suggesting that the maximum rate of Ca2+ uptake during muscle fatigue was strongly depressed. This reduction was larger than expected on the basis of the effects of Pi and pH alone.
Allen,
Intracellular calcium and tension during fatigue in isolated single muscle fibres from Xenopus laevis.
1989, Pubmed,
Xenbase
Allen,
Intracellular calcium and tension during fatigue in isolated single muscle fibres from Xenopus laevis.
1989,
Pubmed
,
Xenbase
Allen,
Muscle cell function during prolonged activity: cellular mechanisms of fatigue.
1995,
Pubmed
Barclay,
Changes in crossbridge and non-crossbridge energetics during moderate fatigue of frog muscle fibres.
1993,
Pubmed
Buschman,
Force-dependent and force-independent heat production in single slow- and fast-twitch muscle fibres from Xenopus laevis.
1996,
Pubmed
,
Xenbase
Byrd,
Altered sarcoplasmic reticulum function after high-intensity exercise.
1989,
Pubmed
Chase,
Effects of pH on contraction of rabbit fast and slow skeletal muscle fibers.
1988,
Pubmed
Curtin,
Effects of carbon dioxide and tetanus duration on relaxation of frog skeletal muscle.
1986,
Pubmed
Dawson,
Mechanical relaxation rate and metabolism studied in fatiguing muscle by phosphorus nuclear magnetic resonance.
1980,
Pubmed
Ebus,
Influence of phosphate and pH on myofibrillar ATPase activity and force in skinned cardiac trabeculae from rat.
1994,
Pubmed
Edman,
Changes in force and stiffness induced by fatigue and intracellular acidification in frog muscle fibres.
1990,
Pubmed
Elzinga,
Stable maintenance heat rate and contractile properties of different single muscle fibres from Xenopus laevis at 20 degrees C.
1987,
Pubmed
,
Xenbase
Fabiato,
Effects of pH on the myofilaments and the sarcoplasmic reticulum of skinned cells from cardiace and skeletal muscles.
1978,
Pubmed
Fabiato,
Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells.
1979,
Pubmed
Fruen,
Regulation of the sarcoplasmic reticulum ryanodine receptor by inorganic phosphate.
1994,
Pubmed
Fryer,
Effects of creatine phosphate and P(i) on Ca2+ movements and tension development in rat skinned skeletal muscle fibres.
1995,
Pubmed
Godt,
Changes of intracellular milieu with fatigue or hypoxia depress contraction of skinned rabbit skeletal and cardiac muscle.
1989,
Pubmed
Goldman,
Control of sarcomere length in skinned muscle fibres of Rana temporaria during mechanical transients.
1984,
Pubmed
Kawai,
The effect of inorganic phosphate on the ATP hydrolysis rate and the tension transients in chemically skinned rabbit psoas fibers.
1987,
Pubmed
Kentish,
The effects of inorganic phosphate and creatine phosphate on force production in skinned muscles from rat ventricle.
1986,
Pubmed
Lamb,
Effect of myoplasmic pH on excitation-contraction coupling in skeletal muscle fibres of the toad.
1992,
Pubmed
MacLennan,
The mechanism of Ca2+ transport by sarco(endo)plasmic reticulum Ca2+-ATPases.
1997,
Pubmed
MacLennan,
Purification and properties of an adenosine triphosphatase from sarcoplasmic reticulum.
1970,
Pubmed
Nagesser,
ATP formation and ATP hydrolysis during fatiguing, intermittent stimulation of different types of single muscle fibres from Xenopus laevis.
1993,
Pubmed
,
Xenbase
Pate,
Reduced effect of pH on skinned rabbit psoas muscle mechanics at high temperatures: implications for fatigue.
1995,
Pubmed
Potma,
Effects of pH on myofibrillar ATPase activity in fast and slow skeletal muscle fibers of the rabbit.
1994,
Pubmed
Potma,
Influence of inorganic phosphate and pH on ATP utilization in fast and slow skeletal muscle fibers.
1995,
Pubmed
Rousseau,
pH modulates conducting and gating behaviour of single calcium release channels.
1990,
Pubmed
Shigekawa,
Reaction mechanism of Ca2+-dependent ATP hydrolysis by skeletal muscle sarcoplasmic reticulum in the absence of added alkali metal salts. I. Characterization of steady state ATP hydrolysis and comparison with that in the presence of KCl.
1978,
Pubmed
Smith,
Inorganic phosphate decreases the Ca2+ content of the sarcoplasmic reticulum in saponin-treated rat cardiac trabeculae.
1992,
Pubmed
Stienen,
Depression of force by phosphate in skinned skeletal muscle fibers of the frog.
1990,
Pubmed
Stienen,
Uptake and caffeine-induced release of calcium in fast muscle fibers of Xenopus laevis: effects of MgATP and P(i).
1993,
Pubmed
,
Xenbase
Stienen,
ATP utilization for calcium uptake and force production in skinned muscle fibres of Xenopus laevis.
1995,
Pubmed
,
Xenbase
Stienen,
Myofibrillar ATPase activity in skinned human skeletal muscle fibres: fibre type and temperature dependence.
1996,
Pubmed
Westerblad,
Slowed relaxation in fatigued skeletal muscle fibers of Xenopus and Mouse. Contribution of [Ca2+]i and cross-bridges.
1997,
Pubmed
,
Xenbase
Westerblad,
The relation between force and intracellular pH in fatigued, single Xenopus muscle fibres.
1988,
Pubmed
,
Xenbase
Wolosker,
pH-dependent inhibitory effects of Ca2+, Mg2+, and K+ on Ca2+ efflux mediated by sarcoplasmic reticulum ATPase.
1994,
Pubmed
Wolosker,
Sarco/endoplasmic reticulum Ca2+-ATPase isoforms: diverse responses to acidosis.
1997,
Pubmed
Zhu,
Intracellular milieu changes associated with hypoxia impair sarcoplasmic reticulum Ca2+ transport in cardiac muscle.
1991,
Pubmed
