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XB-ART-12062
Biochim Biophys Acta 1999 Oct 28;14472-3:334-40.
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Sequence and expression of the monkey homologue of the ER-golgi intermediate compartment lectin, ERGIC-53.

Sarnataro S , Caporaso MG , Bonatti S , Remondelli P .


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We obtained the cDNA sequence of the monkey homologue of the intermediate compartment protein ERGIC-53 by both cDNA library screening and RT-PCR amplification. The final sequence of 2422 nts of the monkey ERGIC-53 cDNA is 96.2% identical to the human ERGIC-53 cDNA and 87% and 67% identical to the rat and amphibian cDNA, respectively. The translated CV1 ERGIC-53 protein is 96.47% identical to the human ERGIC-53, 87% identical to the rat p58 and 66. 98% to the Xenopus laevis protein. Southern blot analysis of multiple genomic DNAs shows the presence of sequences similar to ERGIC-53 in different species. ERGIC-53 is expressed as a major transcript of about 5.5 kb in either monkey CV1 or in human CaCo2. A shorter transcript of 2.3 kb was detected in both cell lines and in mRNAs derived from human pancreas and placenta.

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Species referenced: Xenopus laevis
Genes referenced: lman1 nts prim2