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Molecular cloning and functional characterization of the mouse organic-anion-transporting polypeptide 1 (Oatp1) and mapping of the gene to chromosome X.
Hagenbuch B
,
Adler ID
,
Schmid TE
.
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We have cloned a murine member of the organic-anion-transporting polypeptide (Oatp) family of membrane-transport proteins from mouse liver. The cloned cDNA insert of 2783 bp with an open reading frame of 2011 bp codes for a 12-transmembrane 670-amino-acid protein with highest amino acid identity with the rat Oatp1. When expressed in Xenopus laevis oocytes, the mouse Oatp exhibited the same substrate specificity as the rat Oatp1. Besides the common Oatp substrates bromosulphophthalein, taurocholate, oestrone 3-sulphate and ouabain, the new mouse Oatp also mediates transport of the Oatp1-specific magnetic-resonance-imaging agent gadoxetate. The Oatp2-specific cardiac glycoside digoxin, however, is not transported. Kinetic analyses performed for taurocholate and oestrone 3-sulphate revealed apparent K(m) values of 12 microM and 5 microM respectively. Northern-blot analysis demonstrated a predominant expression in the liver with an additional moderate expression in the kidney. Taken together, the amino acid identity, the functional characteristics and the tissue distribution suggest that we have isolated the murine orthologue of the rat Oatp1, and consequently the identified protein will be called Oatp1. Using fluorescence in situ hybridization, the murine Oatp1 gene was mapped to chromosome XA3-A5.
Abe,
Molecular characterization and tissue distribution of a new organic anion transporter subtype (oatp3) that transports thyroid hormones and taurocholate and comparison with oatp2.
1998, Pubmed
Abe,
Molecular characterization and tissue distribution of a new organic anion transporter subtype (oatp3) that transports thyroid hormones and taurocholate and comparison with oatp2.
1998,
Pubmed
Abe,
Identification of a novel gene family encoding human liver-specific organic anion transporter LST-1.
1999,
Pubmed
Adler,
Spontaneous rates of sex chromosomal aneuploidies in sperm and offspring of mice: a validation of the detection of aneuploid sperm by fluorescence in situ hybridization.
1996,
Pubmed
Angeletti,
The choroid plexus epithelium is the site of the organic anion transport protein in the brain.
1997,
Pubmed
Bergwerk,
Immunologic distribution of an organic anion transport protein in rat liver and kidney.
1996,
Pubmed
,
Xenbase
Bossuyt,
Polyspecific drug and steroid clearance by an organic anion transporter of mammalian liver.
1996,
Pubmed
,
Xenbase
Bossuyt,
Multispecific amphipathic substrate transport by an organic anion transporter of human liver.
1996,
Pubmed
,
Xenbase
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Eckhardt,
Polyspecific substrate uptake by the hepatic organic anion transporter Oatp1 in stably transfected CHO cells.
1999,
Pubmed
,
Xenbase
Eckhardt,
The peptide-based thrombin inhibitor CRC 220 is a new substrate of the basolateral rat liver organic anion-transporting polypeptide.
1996,
Pubmed
,
Xenbase
Friesema,
Identification of thyroid hormone transporters.
1999,
Pubmed
,
Xenbase
Gao,
Localization of the organic anion transporting polypeptide 2 (Oatp2) in capillary endothelium and choroid plexus epithelium of rat brain.
1999,
Pubmed
Hagenbuch,
Functional expression cloning and characterization of the hepatocyte Na+/bile acid cotransport system.
1991,
Pubmed
,
Xenbase
Hagenbuch,
Effect of antisense oligonucleotides on the expression of hepatocellular bile acid and organic anion uptake systems in Xenopus laevis oocytes.
1996,
Pubmed
,
Xenbase
Hagenbuch,
Molecular properties of hepatic uptake systems for bile acids and organic anions.
1997,
Pubmed
Hagenbuch,
Expression of the hepatocyte Na+/bile acid cotransporter in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Ishizuka,
Transport of temocaprilat into rat hepatocytes: role of organic anion transporting polypeptide.
1998,
Pubmed
Jacquemin,
Expression cloning of a rat liver Na(+)-independent organic anion transporter.
1994,
Pubmed
,
Xenbase
Kakyo,
Immunohistochemical distribution and functional characterization of an organic anion transporting polypeptide 2 (oatp2).
1999,
Pubmed
,
Xenbase
Kanai,
Transient expression of oatp organic anion transporter in mammalian cells: identification of candidate substrates.
1996,
Pubmed
,
Xenbase
Kullak-Ublick,
Assignment of the human organic anion transporting polypeptide (OATP) gene to chromosome 12p12 by fluorescence in situ hybridization.
1996,
Pubmed
Kullak-Ublick,
Molecular and functional characterization of an organic anion transporting polypeptide cloned from human liver.
1995,
Pubmed
,
Xenbase
Kurisu,
Preparation of [35S]sulfobromophthalein of high specific activity.
1989,
Pubmed
Meier,
Substrate specificity of sinusoidal bile acid and organic anion uptake systems in rat and human liver.
1997,
Pubmed
Nagase,
Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.
1998,
Pubmed
Noé,
Isolation of a multispecific organic anion and cardiac glycoside transporter from rat brain.
1997,
Pubmed
,
Xenbase
Pang,
The modified dipeptide, enalapril, an angiotensin-converting enzyme inhibitor, is transported by the rat liver organic anion transport protein.
1998,
Pubmed
Reichel,
Localization and function of the organic anion-transporting polypeptide Oatp2 in rat liver.
1999,
Pubmed
,
Xenbase
Saito,
Cloning and functional characterization of a novel rat organic anion transporter mediating basolateral uptake of methotrexate in the kidney.
1996,
Pubmed
van Montfoort,
Polyspecific organic anion transporting polypeptides mediate hepatic uptake of amphipathic type II organic cations.
1999,
Pubmed
,
Xenbase
von Heijne,
Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule.
1992,
Pubmed