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Fibrillarin binds directly and specifically to U16 box C/D snoRNA.
Fatica A
,
Galardi S
,
Altieri F
,
Bozzoni I
.
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Eukaryotic nucleoli contain a large family of box C/D small nucleolar ribonucleoprotein complexes (snoRNPs) that are involved in processing and site-specific methylation of pre-rRNA. Several proteins have been reported to be common factors of box C/D snoRNPs in lower and higher eukaryotes; nevertheless none of them has been clearly shown to directly interact with RNA. We previously identified in Xenopus laevis, by means of UV crosslinking in vivo, two proteins associated with box C/D snoRNAs, fibrillarin and p68. Here we show that fibrillarin interacts directly and specifically with the U16 box C/D snoRNA in a X. laevis oocyte nuclear extract and that it does not require p68 for binding. Specific binding is also obtained with a recombinant fibrillarin demonstrating that the protein is able to bind directly and specifically to U16 snoRNA by itself.
Bagni,
Gar1p binds to the small nucleolar RNAs snR10 and snR30 in vitro through a nontypical RNA binding element.
1998, Pubmed
Bagni,
Gar1p binds to the small nucleolar RNAs snR10 and snR30 in vitro through a nontypical RNA binding element.
1998,
Pubmed
Balakin,
The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions.
1996,
Pubmed
Baserga,
An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs.
1991,
Pubmed
Bortolin,
Elements essential for accumulation and function of small nucleolar RNAs directing site-specific pseudouridylation of ribosomal RNAs.
1999,
Pubmed
Caffarelli,
In vitro study of processing of the intron-encoded U16 small nucleolar RNA in Xenopus laevis.
1994,
Pubmed
,
Xenbase
Caffarelli,
In vivo identification of nuclear factors interacting with the conserved elements of box C/D small nucleolar RNAs.
1998,
Pubmed
,
Xenbase
Caffarelli,
Processing of the intron-encoded U16 and U18 snoRNAs: the conserved C and D boxes control both the processing reaction and the stability of the mature snoRNA.
1996,
Pubmed
,
Xenbase
Cavaillé,
Targeted ribose methylation of RNA in vivo directed by tailored antisense RNA guides.
1996,
Pubmed
Fragapane,
A novel small nucleolar RNA (U16) is encoded inside a ribosomal protein intron and originates by processing of the pre-mRNA.
1993,
Pubmed
,
Xenbase
Ganot,
Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs.
1997,
Pubmed
Ganot,
The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation.
1997,
Pubmed
Gary,
RNA and protein interactions modulated by protein arginine methylation.
1998,
Pubmed
Gautier,
Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis.
1997,
Pubmed
Girard,
GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast.
1992,
Pubmed
Henras,
Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs.
1998,
Pubmed
Jiang,
An essential yeast protein, CBF5p, binds in vitro to centromeres and microtubules.
1993,
Pubmed
Kenan,
RNA recognition: towards identifying determinants of specificity.
1991,
Pubmed
Kiss-László,
Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA.
1998,
Pubmed
Kiss-László,
Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs.
1996,
Pubmed
Lafontaine,
Birth of the snoRNPs: the evolution of the modification-guide snoRNAs.
1998,
Pubmed
Lafontaine,
Nop58p is a common component of the box C+D snoRNPs that is required for snoRNA stability.
1999,
Pubmed
Lafontaine,
The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase.
1998,
Pubmed
Lapeyre,
Molecular cloning of Xenopus fibrillarin, a conserved U3 small nuclear ribonucleoprotein recognized by antisera from humans with autoimmune disease.
1990,
Pubmed
,
Xenbase
Lischwe,
Purification and partial characterization of a nucleolar scleroderma antigen (Mr = 34,000; pI, 8.5) rich in NG,NG-dimethylarginine.
1985,
Pubmed
Maxwell,
The small nucleolar RNAs.
1995,
Pubmed
Meier,
NAP57, a mammalian nucleolar protein with a putative homolog in yeast and bacteria.
1994,
Pubmed
Min,
The generally expressed hnRNP F is involved in a neural-specific pre-mRNA splicing event.
1995,
Pubmed
Ni,
Small nucleolar RNAs direct site-specific synthesis of pseudouridine in ribosomal RNA.
1997,
Pubmed
Nicoloso,
Intron-encoded, antisense small nucleolar RNAs: the characterization of nine novel species points to their direct role as guides for the 2'-O-ribose methylation of rRNAs.
1996,
Pubmed
Niewmierzycka,
S-Adenosylmethionine-dependent methylation in Saccharomyces cerevisiae. Identification of a novel protein arginine methyltransferase.
1999,
Pubmed
Schimmang,
A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability.
1989,
Pubmed
Smith,
Sno storm in the nucleolus: new roles for myriad small RNPs.
1997,
Pubmed
Tollervey,
The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast.
1991,
Pubmed
Tollervey,
Function and synthesis of small nucleolar RNAs.
1997,
Pubmed
Tyc,
U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
1989,
Pubmed
Watkins,
Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure.
1998,
Pubmed
Watkins,
In vitro assembly of the mouse U14 snoRNP core complex and identification of a 65-kDa box C/D-binding protein.
1998,
Pubmed
,
Xenbase
Watkins,
Elements essential for processing intronic U14 snoRNA are located at the termini of the mature snoRNA sequence and include conserved nucleotide boxes C and D.
1996,
Pubmed
,
Xenbase
Wu,
Nop5p is a small nucleolar ribonucleoprotein component required for pre-18 S rRNA processing in yeast.
1998,
Pubmed