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J Bioenerg Biomembr
1999 Dec 01;316:559-67. doi: 10.1023/a:1005417011436.
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Stimulation of mitochondrial gene expression and proliferation of mitochondria following impairment of cellular energy transfer by inhibition of the phosphocreatine circuit in rat hearts.
Wiesner RJ
,
Hornung TV
,
Garman JD
,
Clayton DA
,
O'Gorman E
,
Wallimann T
.
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Mitochondria proliferate when cellular energy demand increases. However, the pathways leading to enhanced expression of mitochondrial genes are largely unknown. We tested the hypothesis that an altered flux through energy metabolism is the key regulatory event by decreasing mitochondrial energy supply to rat heart cells by creatine depletion. Electron microscopy showed that the density of mitochondria increased by 75% in such hearts (p < 0.01). Levels of representative mRNAs encoded on mitochondrial DNA (mtDNA) or on nuclear chromosomes were elevated 1.5 to 2-fold (p < 0.05), while the mtDNA content was unchanged. The mRNA for the nuclear encoded mitochondrial transcription factor A (mtTFA) was increased after GPA feeding (p < 0.05). Thus, we have shown that an impairment of mitochondrial energy supply causes stimulation of gene expression resulting in mitochondrial proliferation, probably as a compensatory mechanism. The observed activation of the mtTFA gene corroborates the important function of this protein in nuclear-mitochondrial communication.
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976, Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
COOPERSTEIN,
A microspectrophotometric method for the determination of cytochrome oxidase.
1951,
Pubmed
Cantatore,
Identification by in Organello footprinting of protein contact sites and of single-stranded DNA sequences in the regulatory region of rat mitochondrial DNA. Protein binding sites and single-stranded DNA regions in isolated rat liver mitochondria.
1995,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Fisher,
Flexible recognition of rapidly evolving promoter sequences by mitochondrial transcription factor 1.
1989,
Pubmed
Garstka,
Stoichiometry of mitochondrial transcripts and regulation of gene expression by mitochondrial transcription factor A.
1994,
Pubmed
,
Xenbase
Ghivizzani,
In organello footprint analysis of human mitochondrial DNA: human mitochondrial transcription factor A interactions at the origin of replication.
1994,
Pubmed
,
Xenbase
Graham,
A mouse model for mitochondrial myopathy and cardiomyopathy resulting from a deficiency in the heart/muscle isoform of the adenine nucleotide translocator.
1997,
Pubmed
Green,
Metabolite patterns related to exhaustion, recovery and transformation of chronically stimulated rabbit fast-twitch muscle.
1992,
Pubmed
Harting,
Quantification of transcript-to-template ratios as a measure of gene expression using RT-PCR.
1997,
Pubmed
Holtzman,
In vivo brain phosphocreatine and ATP regulation in mice fed a creatine analog.
1997,
Pubmed
Ismail-Beigi,
Thyroid hormone regulation of Na,K-ATPase expression.
1993,
Pubmed
Izquierdo,
Changing patterns of transcriptional and post-transcriptional control of beta-F1-ATPase gene expression during mitochondrial biogenesis in liver.
1995,
Pubmed
Kemp,
Dealing with energy demand: the AMP-activated protein kinase.
1999,
Pubmed
Klingenspor,
Biogenesis of thermogenic mitochondria in brown adipose tissue of Djungarian hamsters during cold adaptation.
1996,
Pubmed
Kubis,
Adult fast myosin pattern and Ca2+-induced slow myosin pattern in primary skeletal muscle culture.
1997,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Larsson,
Mitochondrial transcription factor A is necessary for mtDNA maintenance and embryogenesis in mice.
1998,
Pubmed
Mekhfi,
Myocardial adaptation to creatine deficiency in rats fed with beta-guanidinopropionic acid, a creatine analogue.
1990,
Pubmed
Montoya,
Regulation of mitochondrial transcription by mitochondrial transcription factor A.
1997,
Pubmed
,
Xenbase
O'Gorman,
Differential effects of creatine depletion on the regulation of enzyme activities and on creatine-stimulated mitochondrial respiration in skeletal muscle, heart, and brain.
1996,
Pubmed
O'Gorman,
Mitochondrial intermembrane inclusion bodies: the common denominator between human mitochondrial myopathies and creatine depletion, due to impairment of cellular energetics.
1997,
Pubmed
Pette,
Adaptation of mammalian skeletal muscle fibers to chronic electrical stimulation.
1992,
Pubmed
Ponticos,
Dual regulation of the AMP-activated protein kinase provides a novel mechanism for the control of creatine kinase in skeletal muscle.
1998,
Pubmed
Robin,
Mitochondrial DNA molecules and virtual number of mitochondria per cell in mammalian cells.
1988,
Pubmed
Rolfe,
Cellular energy utilization and molecular origin of standard metabolic rate in mammals.
1997,
Pubmed
Shadel,
Mitochondrial DNA maintenance in vertebrates.
1997,
Pubmed
Stadhouders,
Mitochondrial creatine kinase: a major constituent of pathological inclusions seen in mitochondrial myopathies.
1994,
Pubmed
Steeghs,
Altered Ca2+ responses in muscles with combined mitochondrial and cytosolic creatine kinase deficiencies.
1997,
Pubmed
Van Itallie,
Dexamethasone treatment increases mitochondrial RNA synthesis in a rat hepatoma cell line.
1992,
Pubmed
Virbasius,
The rat cytochrome c oxidase subunit IV gene family: tissue-specific and hormonal differences in subunit IV and cytochrome c mRNA expression.
1990,
Pubmed
Wallimann,
Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands: the 'phosphocreatine circuit' for cellular energy homeostasis.
1992,
Pubmed
Wallimann,
Some new aspects of creatine kinase (CK): compartmentation, structure, function and regulation for cellular and mitochondrial bioenergetics and physiology.
1998,
Pubmed
Weber,
A new mtDNA mutation showing accumulation with time and restriction to skeletal muscle.
1997,
Pubmed
Wiesner,
Regulation of mitochondrial gene expression: transcription versus replication.
1992,
Pubmed
Wiesner,
Regulation by thyroid hormone of nuclear and mitochondrial genes encoding subunits of cytochrome-c oxidase in rat liver and skeletal muscle.
1992,
Pubmed
Wiesner,
Counting target molecules by exponential polymerase chain reaction: copy number of mitochondrial DNA in rat tissues.
1992,
Pubmed
Wyss,
Creatine metabolism and the consequences of creatine depletion in muscle.
1994,
Pubmed
