Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Neurosci
2005 Nov 16;2546:10617-26. doi: 10.1523/JNEUROSCI.2015-05.2005.
Show Gene links
Show Anatomy links
Identification of molluscan nicotinic acetylcholine receptor (nAChR) subunits involved in formation of cation- and anion-selective nAChRs.
van Nierop P
,
Keramidas A
,
Bertrand S
,
van Minnen J
,
Gouwenberg Y
,
Bertrand D
,
Smit AB
.
???displayArticle.abstract???
Acetylcholine (ACh) is a neurotransmitter commonly found in all animal species. It was shown to mediate fast excitatory and inhibitory neurotransmission in the molluscan CNS. Since early intracellular recordings, it was shown that the receptors mediating these currents belong to the family of neuronal nicotinic acetylcholine receptors and that they can be distinguished on the basis of their pharmacology. We previously identified 12 Lymnaea cDNAs that were predicted to encode ion channel subunits of the family of the neuronal nicotinic acetylcholine receptors. These Lymnaea nAChRs can be subdivided in groups according to the residues supposedly contributing to the selectivity of ion conductance. Functional analysis in Xenopus oocytes revealed that two types of subunits with predicted distinct ion selectivities form homopentameric nicotinic ACh receptor (nAChR) subtypes conducting either cations or anions. Phylogenetic analysis of the nAChR gene sequences suggests that molluscan anionic nAChRs probably evolved from cationic ancestors through amino acid substitutions in the ion channel pore, a mechanism different from acetylcholine-gated channels in other invertebrates.
Alkondon,
Blockade of nicotinic currents in hippocampal neurons defines methyllycaconitine as a potent and specific receptor antagonist.
1992, Pubmed
Alkondon,
Blockade of nicotinic currents in hippocampal neurons defines methyllycaconitine as a potent and specific receptor antagonist.
1992,
Pubmed
Alkondon,
Choline is a selective agonist of alpha7 nicotinic acetylcholine receptors in the rat brain neurons.
1997,
Pubmed
Barry,
JPCalc, a software package for calculating liquid junction potential corrections in patch-clamp, intracellular, epithelial and bilayer measurements and for correcting junction potential measurements.
1994,
Pubmed
Beg,
EXP-1 is an excitatory GABA-gated cation channel.
2003,
Pubmed
,
Xenbase
Bertrand,
Activation and blocking of neuronal nicotinic acetylcholine receptor reconstituted in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Bhandal,
Channel gating in the absence of agonist by a homo-oligomeric molluscan GABA receptor expressed in Xenopus oocytes from a cloned cDNA.
1995,
Pubmed
,
Xenbase
Bouzat,
Coupling of agonist binding to channel gating in an ACh-binding protein linked to an ion channel.
2004,
Pubmed
Buisson,
Chronic exposure to nicotine upregulates the human (alpha)4((beta)2 nicotinic acetylcholine receptor function.
2001,
Pubmed
Chemeris,
Inhibition of acetylcholine responses by intracellular calcium in Lymnaea stagnalis neurones.
1982,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Corringer,
Mutational analysis of the charge selectivity filter of the alpha7 nicotinic acetylcholine receptor.
1999,
Pubmed
,
Xenbase
Costa,
Determination of ionic permeability coefficients of the plasma membrane of Xenopus laevis oocytes under voltage clamp.
1989,
Pubmed
,
Xenbase
Couturier,
A neuronal nicotinic acetylcholine receptor subunit (alpha 7) is developmentally regulated and forms a homo-oligomeric channel blocked by alpha-BTX.
1990,
Pubmed
,
Xenbase
Covernton,
Multiple components in the agonist concentration-response relationships of neuronal nicotinic acetylcholine receptors.
2000,
Pubmed
,
Xenbase
Cully,
Cloning of an avermectin-sensitive glutamate-gated chloride channel from Caenorhabditis elegans.
1994,
Pubmed
,
Xenbase
Cully,
Identification of a Drosophila melanogaster glutamate-gated chloride channel sensitive to the antiparasitic agent avermectin.
1996,
Pubmed
,
Xenbase
Davies,
A novel class of ligand-gated ion channel is activated by Zn2+.
2003,
Pubmed
Eiselé,
Chimaeric nicotinic-serotonergic receptor combines distinct ligand binding and channel specificities.
1993,
Pubmed
,
Xenbase
Elgoyhen,
alpha10: a determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells.
2001,
Pubmed
,
Xenbase
Elgoyhen,
Alpha 9: an acetylcholine receptor with novel pharmacological properties expressed in rat cochlear hair cells.
1994,
Pubmed
,
Xenbase
Galzi,
Mutations in the channel domain of a neuronal nicotinic receptor convert ion selectivity from cationic to anionic.
1992,
Pubmed
,
Xenbase
Gerzanich,
Homomers of alpha 8 and alpha 7 subunits of nicotinic receptors exhibit similar channel but contrasting binding site properties.
1994,
Pubmed
,
Xenbase
Gisselmann,
Two cDNAs coding for histamine-gated ion channels in D. melanogaster.
2002,
Pubmed
,
Xenbase
Gisselmann,
Unusual functional properties of homo- and heteromultimeric histamine-gated chloride channels of Drosophila melanogaster: spontaneous currents and dual gating by GABA and histamine.
2004,
Pubmed
,
Xenbase
Gunthorpe,
Conversion of the ion selectivity of the 5-HT(3a) receptor from cationic to anionic reveals a conserved feature of the ligand-gated ion channel superfamily.
2001,
Pubmed
Horoszok,
GLC-3: a novel fipronil and BIDN-sensitive, but picrotoxinin-insensitive, L-glutamate-gated chloride channel subunit from Caenorhabditis elegans.
2001,
Pubmed
,
Xenbase
Jensen,
The beta subunit determines the ion selectivity of the GABAA receptor.
2002,
Pubmed
Jensen,
Charge selectivity of the Cys-loop family of ligand-gated ion channels.
2005,
Pubmed
Jensen,
Mutational studies using a cation-conducting GABAA receptor reveal the selectivity determinants of the Cys-loop family of ligand-gated ion channels.
2005,
Pubmed
Johnson,
alpha-Conotoxin ImI exhibits subtype-specific nicotinic acetylcholine receptor blockade: preferential inhibition of homomeric alpha 7 and alpha 9 receptors.
1995,
Pubmed
,
Xenbase
Kehoe,
Effects of alpha-toxins from Bungarus multicinctus and Bungarus caeruleus on cholinergic responses in Aplysia neurons.
1976,
Pubmed
Kehoe,
Ionic mechanisms of a two-component cholinergic inhibition in Aplysia neurones.
1972,
Pubmed
Kehoe,
Three acetylcholine receptors in Aplysia neurones.
1972,
Pubmed
Kehoe,
Two distinct nicotinic receptors, one pharmacologically similar to the vertebrate alpha7-containing receptor, mediate Cl currents in aplysia neurons.
1998,
Pubmed
Keramidas,
M2 pore mutations convert the glycine receptor channel from being anion- to cation-selective.
2000,
Pubmed
Keramidas,
Cation-selective mutations in the M2 domain of the inhibitory glycine receptor channel reveal determinants of ion-charge selectivity.
2002,
Pubmed
Keramidas,
Ligand-gated ion channels: mechanisms underlying ion selectivity.
2004,
Pubmed
Koert,
Functional implications of neurotransmitter expression during axonal regeneration: serotonin, but not peptides, auto-regulate axon growth of an identified central neuron.
2001,
Pubmed
Le Novère,
Molecular evolution of the nicotinic acetylcholine receptor: an example of multigene family in excitable cells.
1995,
Pubmed
Orr-Urtreger,
Mice deficient in the alpha7 neuronal nicotinic acetylcholine receptor lack alpha-bungarotoxin binding sites and hippocampal fast nicotinic currents.
1997,
Pubmed
Ortells,
Evolutionary history of the ligand-gated ion-channel superfamily of receptors.
1995,
Pubmed
Palma,
Neuronal nicotinic alpha 7 receptor expressed in Xenopus oocytes presents five putative binding sites for methyllycaconitine.
1996,
Pubmed
,
Xenbase
Papke,
An evaluation of neuronal nicotinic acetylcholine receptor activation by quaternary nitrogen compounds indicates that choline is selective for the alpha 7 subtype.
1996,
Pubmed
,
Xenbase
Putrenko,
A family of acetylcholine-gated chloride channel subunits in Caenorhabditis elegans.
2005,
Pubmed
Ranganathan,
MOD-1 is a serotonin-gated chloride channel that modulates locomotory behaviour in C. elegans.
2000,
Pubmed
,
Xenbase
Schoepfer,
Brain alpha-bungarotoxin binding protein cDNAs and MAbs reveal subtypes of this branch of the ligand-gated ion channel gene superfamily.
1990,
Pubmed
Syed,
In vitro reconstruction of the respiratory central pattern generator of the mollusk Lymnaea.
1990,
Pubmed
TAUC,
A cholinergic mechanism of inhibitory synaptic transmission in a molluscan nervous system.
1962,
Pubmed
Thompson,
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
1997,
Pubmed
Verbitsky,
Mixed nicotinic-muscarinic properties of the alpha9 nicotinic cholinergic receptor.
2000,
Pubmed
,
Xenbase
Vulfius,
Diversity of nicotinic receptors mediating Cl- current in Lymnaea neurons distinguished with specific agonists and antagonist.
2005,
Pubmed
Vulfius,
Arrangement of cholinoreceptors on the neuronal membrane of two pulmonate gastropods.
1967,
Pubmed
Wachtel,
Conversion of synaptic excitation to inhibition at a dual chemical synapse.
1971,
Pubmed
Ward,
Methyllycaconitine: a selective probe for neuronal alpha-bungarotoxin binding sites.
1990,
Pubmed
Weber,
Ion currents of Xenopus laevis oocytes: state of the art.
1999,
Pubmed
,
Xenbase
Woodin,
Trophic factor-induced excitatory synaptogenesis involves postsynaptic modulation of nicotinic acetylcholine receptors.
2002,
Pubmed
Wotring,
Mutations at the GABA receptor selectivity filter: a possible role for effective charges.
2003,
Pubmed
,
Xenbase
Yeoman,
A cholinergic modulatory interneuron in the feeding system of the snail, Lymnaea.
1993,
Pubmed