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Red-cell glycophorin A-band 3 interactions associated with the movement of band 3 to the cell surface.
Young MT
,
Beckmann R
,
Toye AM
,
Tanner MJ
.
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We have examined the mechanism by which glycophorin A (GPA) facilitates the movement of the human red-cell anion exchanger (band 3, AE1) to the cell surface. GPA itself forms stable dimers in membranes and detergent solution. Four mutants of human GPA with impaired dimerization were prepared (L75I, I76A, G79L and G83L). All four GPA mutants enhanced band 3 translocation to the Xenopus oocyteplasma membrane in the same way as wild-type GPA, showing that the GPA monomer is sufficient to mediate this process. Cell-surface expression of the natural band 3 mutant G701D has an absolute requirement for GPA. GPA monomers also rescued the cell-surface expression of this mutant band 3. Taking into account other evidence, we infer that the site of GPA interaction with band 3 is located outside the GPA dimerization interface but within the GPA transmembrane span. The results of examination of the cell-surface expression of GPA and band 3 in different K562 erythroleukaemia cell clones stably transfected with band 3 are consistent with the movement of GPA and band 3 to the cell surface together. We discuss the pathways by which band 3 moves to the cell surface in the presence and the absence of GPA, concluding that GPA has a role in enhancing the folding and maturation of band 3. We propose that GPA functions in erythroid cells to assist with the incorporation of large amounts of properly folded band 3 into the membrane within a limited time span during erythroid maturation.
Alder,
A kinetic and clonal analysis of heterogeneity in K562 cells.
1984, Pubmed
Alder,
A kinetic and clonal analysis of heterogeneity in K562 cells.
1984,
Pubmed
Andersson,
Glycophorin A as a cell surface marker of early erythroid differentiation in acute leukemia.
1979,
Pubmed
Anstee,
Monoclonal antibodies to human erythrocytes.
1982,
Pubmed
Anstee,
Blood group-active surface molecules of the human red blood cell.
1990,
Pubmed
Beckmann,
Functional cell surface expression of band 3, the human red blood cell anion exchange protein (AE1), in K562 erythroleukemia cells: band 3 enhances the cell surface reactivity of Rh antigens.
1998,
Pubmed
Brosig,
The dimerization motif of the glycophorin A transmembrane segment in membranes: importance of glycine residues.
1998,
Pubmed
Bruce,
Band 3 mutations, renal tubular acidosis and South-East Asian ovalocytosis in Malaysia and Papua New Guinea: loss of up to 95% band 3 transport in red cells.
2000,
Pubmed
,
Xenbase
Bruce,
Altered band 3 structure and function in glycophorin A- and B-deficient (MkMk) red blood cells.
1994,
Pubmed
Bruce,
Changes in the blood group Wright antigens are associated with a mutation at amino acid 658 in human erythrocyte band 3: a site of interaction between band 3 and glycophorin A under certain conditions.
1995,
Pubmed
Cartron,
Human erythrocyte glycophorins: protein and gene structure analyses.
1992,
Pubmed
Casey,
Analysis of the oligomeric state of Band 3, the anion transport protein of the human erythrocyte membrane, by size exclusion high performance liquid chromatography. Oligomeric stability and origin of heterogeneity.
1991,
Pubmed
Casey,
The role of cysteine residues in the erythrocyte plasma membrane anion exchange protein, AE1.
1995,
Pubmed
Che,
Loss of rotational mobility of band 3 proteins in human erythrocyte membranes induced by antibodies to glycophorin A.
1995,
Pubmed
Dahr,
Heterogeneity of human cell membrane sialoglycoproteins.
1976,
Pubmed
Dahr,
The Dantu erythrocyte phenotype of the NE variety. I. Dodecylsulfate polyacrylamide gel electrophoretic studies.
1987,
Pubmed
Duk,
Isolation and characterization of glycophorin from nucleated (chicken) erythrocytes.
2000,
Pubmed
Fleming,
The effect of point mutations on the free energy of transmembrane alpha-helix dimerization.
1997,
Pubmed
Gahmberg,
Absence of the major sialoglycoprotein in the membrane of human En(a--) erythrocytes and increased glycosylation of band 3.
1976,
Pubmed
Ghosh,
Chicken erythroid AE1 anion exchangers associate with the cytoskeleton during recycling to the Golgi.
1999,
Pubmed
Grinstein,
Anion transport in relation to proteolytic dissection of band 3 protein.
1978,
Pubmed
Groves,
Glycophorin A facilitates the expression of human band 3-mediated anion transport in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Groves,
Role of N-glycosylation in the expression of human band 3-mediated anion transport.
1994,
Pubmed
,
Xenbase
Groves,
The effects of glycophorin A on the expression of the human red cell anion transporter (band 3) in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Groves,
Co-expressed complementary fragments of the human red cell anion exchanger (band 3, AE1) generate stilbene disulfonate-sensitive anion transport.
1995,
Pubmed
,
Xenbase
Groves,
Heterologous expression of the red-cell anion exchanger (band 3; AE1).
1999,
Pubmed
,
Xenbase
Hassoun,
Complete deficiency of glycophorin A in red blood cells from mice with targeted inactivation of the band 3 (AE1) gene.
1998,
Pubmed
Jennings,
Structure and function of the red blood cell anion transport protein.
1989,
Pubmed
Kollert-Jöns,
Anion exchanger 1 in human kidney and oncocytoma differs from erythroid AE1 in its NH2 terminus.
1993,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Langosch,
Dimerisation of the glycophorin A transmembrane segment in membranes probed with the ToxR transcription activator.
1996,
Pubmed
Lemmon,
Sequence specificity in the dimerization of transmembrane alpha-helices.
1992,
Pubmed
Lepke,
Effects of incorporated trypsin on anion exchange and membrane proteins in human red blood cell ghosts.
1976,
Pubmed
Lepke,
Mediation of inorganic anion transport by the hydrophobic domain of mouse erythroid band 3 protein expressed in oocytes of Xenopus laevis.
1992,
Pubmed
,
Xenbase
Low,
Structure and function of the cytoplasmic domain of band 3: center of erythrocyte membrane-peripheral protein interactions.
1986,
Pubmed
MacKenzie,
A transmembrane helix dimer: structure and implications.
1997,
Pubmed
Marton,
Subunit structure of the major human erythrocytes glycoprotein: depolymerization by heating ghosts with sodium dodecyl sulfate.
1973,
Pubmed
Mingarro,
Helix-helix packing in a membrane-like environment.
1997,
Pubmed
Morgenstern,
Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.
1990,
Pubmed
Nigg,
Band 3-glycophorin A association in erythrocyte membrane demonstrated by combining protein diffusion measurements with antibody-induced cross-linking.
1980,
Pubmed
Padgett,
Creating seamless junctions independent of restriction sites in PCR cloning.
1996,
Pubmed
Ridgwell,
The Wrb antigen, a receptor for Plasmodium falciparum malaria, is located on a helical region of the major membrane sialoglycoprotein of human red blood cells.
1983,
Pubmed
Ring,
Comparative immunochemical analysis of Wra and Wrb red cell antigens.
1994,
Pubmed
Russ,
TOXCAT: a measure of transmembrane helix association in a biological membrane.
1999,
Pubmed
Schägger,
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
1987,
Pubmed
Smythe,
Monoclonal antibodies recognizing epitopes on the extracellular face and intracellular N-terminus of the human erythrocyte anion transporter (band 3) and their application to the analysis of South East Asian ovalocytes.
1995,
Pubmed
Southcott,
The expression of human blood group antigens during erythropoiesis in a cell culture system.
1999,
Pubmed
Steck,
The band 3 protein of the human red cell membrane: a review.
1978,
Pubmed
Tanner,
Abnormal carbohydrate composition of the major penetrating membrane protein of En(a-) human erythrocytes.
1976,
Pubmed
Tanner,
Molecular and cellular biology of the erythrocyte anion exchanger (AE1).
1993,
Pubmed
Tanner,
The structure and function of band 3 (AE1): recent developments (review).
1997,
Pubmed
Tanphaichitr,
Novel AE1 mutations in recessive distal renal tubular acidosis. Loss-of-function is rescued by glycophorin A.
1998,
Pubmed
,
Xenbase
Telen,
Relationship of the human erythrocyte Wrb antigen to an interaction between glycophorin A and band 3.
1990,
Pubmed
Timmer,
Inducible expression of erythrocyte band 3 protein.
1999,
Pubmed
Tomita,
Amino-acid sequence and oligosaccharide attachment sites of human erythrocyte glycophorin.
1975,
Pubmed
Wagner,
Immunochemical characterization of a band 3-like anion exchanger in collecting duct of human kidney.
1987,
Pubmed
