Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biochem
2000 May 01;2081-2:99-109. doi: 10.1023/a:1007098122583.
Show Gene links
Show Anatomy links
Evolutionary conservation of post-transcriptional 3' end adenylation of small RNAs: S. cerevisiae signal recognition particle RNA and U2 small nuclear RNA are post-transcriptionally adenylated.
Perumal K
,
Gu J
,
Reddy R
.
???displayArticle.abstract???
The 3' terminal nucleotide of several human small RNAs, including Signal Recognition Particle (SRP) RNA, 7SK RNA, U2 small nuclear RNA and ribosomal 5S RNA was previously characterized and a fraction of these RNAs was found to contain a single post-transcriptionally added adenylic acid residue on their 3' ends. Here we report the development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for determining and quantifying the extent of post-transcriptional adenylation of RNAs from different species. Using this assay, we found that a fraction of S. cerevisiae U2 small nuclear RNA and S. cerevisiae SRP RNA contain a post-transcriptionally added adenylic acid residue on their 3' ends. Sequencing analysis confirmed this adenylation to be post-transcriptional. Corresponding small RNAs in Xenopus oocytes also contained this post-transcriptional adenylation on their 3' ends. These data show that post-transcriptional adenylation on the 3' end of several small RNA molecules is conserved through evolution. Xenopus SRP RNA from both cytoplasmic and nuclear compartments contained post-transcriptionally added adenylic acid residue on its 3' end. In addition, the Alu portion of SRP RNA was adenylated, when injected into the cytoplasm of frog oocytes. These data show that this novel adenylating machinery, capable of specifically adding a single adenylic acid to the 3' end of some RNA molecules, is present and functional in both nucleus and cytoplasm.
Abou Elela,
Depletion of yeast RNase III blocks correct U2 3' end formation and results in polyadenylated but functional U2 snRNA.
1998, Pubmed
Abou Elela,
Depletion of yeast RNase III blocks correct U2 3' end formation and results in polyadenylated but functional U2 snRNA.
1998,
Pubmed
Ares,
U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs.
1986,
Pubmed
Ballantyne,
Poly (A) polymerases in the nucleus and cytoplasm of frog oocytes: dynamic changes during oocyte maturation and early development.
1995,
Pubmed
,
Xenbase
Bausch,
Terminal adenylation in the synthesis of RNA by Q beta replicase.
1983,
Pubmed
Birse,
The crystal structure of the signal recognition particle Alu RNA binding heterodimer, SRP9/14.
1997,
Pubmed
Black,
U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing.
1985,
Pubmed
Blum,
Guide RNAs in kinetoplastid mitochondria have a nonencoded 3' oligo(U) tail involved in recognition of the preedited region.
1990,
Pubmed
Byrne,
RNA-protein interactions in the ribonucleoprotein T-complexes in a mitochondrial extract from Leishmania tarentolae.
1995,
Pubmed
Chapon,
Polyadenylation of telomerase RNA in budding yeast.
1997,
Pubmed
Chen,
Accurate 3' end processing and adenylation of human signal recognition particle RNA and alu RNA in vitro.
1998,
Pubmed
Deutscher,
Ribonucleases, tRNA nucleotidyltransferase, and the 3' processing of tRNA.
1990,
Pubmed
Edmonds,
Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship.
1971,
Pubmed
England,
Specific labeling of 3' termini of RNA with T4 RNA ligase.
1980,
Pubmed
Felici,
The most abundant small cytoplasmic RNA of Saccharomyces cerevisiae has an important function required for normal cell growth.
1989,
Pubmed
He,
Nuclear export of signal recognition particle RNA is a facilitated process that involves the Alu sequence domain.
1994,
Pubmed
,
Xenbase
Jacobson,
Localization of signal recognition particle RNA in the nucleolus of mammalian cells.
1998,
Pubmed
Keller,
No end yet to messenger RNA 3' processing!
1995,
Pubmed
Kramerov,
Nucleotide sequence of small polyadenylated B2 RNA.
1985,
Pubmed
Lund,
Proofreading and aminoacylation of tRNAs before export from the nucleus.
1998,
Pubmed
,
Xenbase
Manley,
A complex protein assembly catalyzes polyadenylation of mRNA precursors.
1995,
Pubmed
McGrew,
Translational control by cytoplasmic polyadenylation during Xenopus oocyte maturation: characterization of cis and trans elements and regulation by cyclin/MPF.
1990,
Pubmed
,
Xenbase
Nakazato,
Polyadenylic acid sequences in E. coli messenger RNA.
1975,
Pubmed
O'Brien,
A possible role for the 60-kD Ro autoantigen in a discard pathway for defective 5S rRNA precursors.
1994,
Pubmed
,
Xenbase
Parker,
Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA.
1987,
Pubmed
Piper,
Altered maturation of sequences at the 3' terminus of 5S gene transcripts in a Saccharomyces cerevisiae mutant that lacks a RNA processing endonuclease.
1983,
Pubmed
Reddy,
Characterization and subcellular localization of 7-8 S RNAs of Novikoff hepatoma.
1981,
Pubmed
Rinke,
Association of the lupus antigen La with a subset of U6 snRNA molecules.
1985,
Pubmed
Sachs,
Poly(A) tail metabolism and function in eucaryotes.
1993,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sinha,
Adenylation of small RNAs in human cells. Development of a cell-free system for accurate adenylation on the 3'-end of human signal recognition particle RNA.
1998,
Pubmed
Terns,
Nuclear transport of RNAs in microinjected Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Tessier,
Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase.
1986,
Pubmed
Trippe,
A highly specific terminal uridylyl transferase modifies the 3'-end of U6 small nuclear RNA.
1998,
Pubmed
Ullu,
Human genes and pseudogenes for the 7SL RNA component of signal recognition particle.
1984,
Pubmed
Wahle,
The biochemistry of 3'-end cleavage and polyadenylation of messenger RNA precursors.
1992,
Pubmed
Walter,
Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum.
1982,
Pubmed
Walter,
Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane.
1994,
Pubmed
Weichenrieder,
Identification of a minimal Alu RNA folding domain that specifically binds SRP9/14.
1997,
Pubmed
Xu,
RNA degradation in Escherichia coli regulated by 3' adenylation and 5' phosphorylation.
1995,
Pubmed
Yue,
CCA-adding enzymes and poly(A) polymerases are all members of the same nucleotidyltransferase superfamily: characterization of the CCA-adding enzyme from the archaeal hyperthermophile Sulfolobus shibatae.
1996,
Pubmed
Zaug,
Method for determining RNA 3' ends and application to human telomerase RNA.
1996,
Pubmed