Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Biotechnol
2000 Jun 01;152:167-84. doi: 10.1385/MB:15:2:167.
Show Gene links
Show Anatomy links
Visualization of DNA and RNA molecules, and protein-DNA complexes for electron microscopy.
Hajibagheri MA
.
???displayArticle.abstract???
This article provides step-by step instructions for the preparation of double- and single-stranded DNA and RNA molecules and protein-DNA complexes for electron microscopy (EM). Absorption, spreading, staining, dark-field imaging, and metal shadowing techniques are described in detail. A number of examples are illustrated on analysis of DNA replication, DNA repair and DNA recombination to demonstrate the usefulness of the technique for EM visualisation. Application of immunogold labeling of specific protein in DNA-protein complexes is also covered.
Banerjee,
Quick and easy spreading technique for electron microscopy of DNA.
1995, Pubmed
Banerjee,
Quick and easy spreading technique for electron microscopy of DNA.
1995,
Pubmed
Bazett-Jones,
DNA organization in nucleosomes.
1982,
Pubmed
Dubochet,
A new preparation method for dark-field electron microscopy of biomacromolecules.
1971,
Pubmed
Glass,
Different base per unit length ratios exist in single-stranded RNA and single-stranded DNA.
1980,
Pubmed
Griffith,
Electron microscope visualization of chromatin and other DNA-protein complexes.
1978,
Pubmed
Griffith,
DNA structure: evidence from electron microscopy.
1978,
Pubmed
Hewlett,
Circular forms of Uukuniemi virion RNA: an electron microscopic study.
1977,
Pubmed
Katsani,
Co-operative DNA binding by GAGA transcription factor requires the conserved BTB/POZ domain and reorganizes promoter topology.
1999,
Pubmed
Lang,
Simplified quantitative electron microscopy of biopolymers.
1970,
Pubmed
Masson,
The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.
1999,
Pubmed
Parsons,
Structure of a multisubunit complex that promotes DNA branch migration.
1995,
Pubmed
Poon,
Fine structure of mononucleosomes derived by bright- and dark-field electron microscopy.
1980,
Pubmed
Preiser,
Recombination associated with replication of malarial mitochondrial DNA.
1996,
Pubmed
Revet,
Electron microscopic measurement of chain flexibility of poly(dG-dC).poly(dG-dC) modified by cis-diamminedichloroplatinum(II).
1984,
Pubmed
Ruben,
Relaxed circular SV40 DNA as cleavage intermediate of two restriction endonucleases.
1977,
Pubmed
Stasiak,
Structure and function of RecA-DNA complexes.
1994,
Pubmed
Szymkowski,
Electron microscopy of DNA excision repair patches produced by human cell extracts.
1993,
Pubmed
Van Dyck,
Visualisation of human rad52 protein and its complexes with hRad51 and DNA.
1998,
Pubmed
Vollenweider,
A routine method for protein-free spreading of double- and single-stranded nucleic acid molecules.
1975,
Pubmed
Wessel,
The interaction of SV40 large T antigen with unspecific double-stranded DNA: an electron microscopic study.
1992,
Pubmed
Wessel,
Electron microscopy of DNA.helicase-I complexes in the act of strand separation.
1990,
Pubmed
de Murcia,
Poly(ADP-ribose) polymerase auto-modification and interaction with DNA: electron microscopic visualization.
1983,
Pubmed
van Gool,
Assembly of the Escherichia coli RuvABC resolvasome directs the orientation of holliday junction resolution.
1999,
Pubmed
