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Role of cationic amino acids in the Na+/dicarboxylate co-transporter NaDC-1.
Pajor AM
,
Kahn ES
,
Gangula R
.
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The role of cationic amino acids in the Na(+)/dicarboxylate co-transporter NaDC-1 was investigated by site-directed mutagenesis and subsequent expression of mutant transporters in Xenopus oocytes. Of the ten residues chosen for mutagenesis, eight (Lys-34, Lys-107, Arg-108, Lys-333, Lys-390, Arg-368, Lys-414 and Arg-541) were found to be non-essential for function or targeting. Only two conserved residues, Lys-84 (at the cytoplasmic end of helix 3) and Arg-349 (at the extracellular end of helix 7), were found to be important for transport. Both mutant transporters were expressed at the plasma membrane. The mutation of Lys-84 to Ala resulted in an increased K(m) for succinate of 1.8 mM, compared with 0.3 mM in the wild-type NaDC-1. The R349A mutant had Na(+) and citrate kinetics that were similar to those of the wild type. However, succinate handling in the R349A mutant was altered, with evidence of inhibition at high succinate concentrations. In conclusion, charge neutralization of Lys-84 and Arg-349 in NaDC-1 affects succinate handling, suggesting that these residues might have roles in substrate binding.
Bai,
Expression cloning of NaDC-2, an intestinal Na(+)- or Li(+)-dependent dicarboxylate transporter.
1997, Pubmed,
Xenbase
Bai,
Expression cloning of NaDC-2, an intestinal Na(+)- or Li(+)-dependent dicarboxylate transporter.
1997,
Pubmed
,
Xenbase
Chang,
Removal of multiple arginine-framed trafficking signals overcomes misprocessing of delta F508 CFTR present in most patients with cystic fibrosis.
1999,
Pubmed
Chen,
Molecular and functional analysis of SDCT2, a novel rat sodium-dependent dicarboxylate transporter.
1999,
Pubmed
,
Xenbase
Conradt,
Functional analysis of the high affinity, Na(+)-dependent glutamate transporter GLAST-1 by site-directed mutagenesis.
1995,
Pubmed
,
Xenbase
Frillingos,
Cys-scanning mutagenesis: a novel approach to structure function relationships in polytopic membrane proteins.
1998,
Pubmed
Girard,
Molecular cloning and functional analysis of SUT-1, a sulfate transporter from human high endothelial venules.
1999,
Pubmed
,
Xenbase
Gouaux,
Three-dimensional structure of carbamoyl phosphate and succinate bound to aspartate carbamoyltransferase.
1988,
Pubmed
Griffith,
Acidic residues involved in cation and substrate interactions in the Na+/dicarboxylate cotransporter, NaDC-1.
1999,
Pubmed
,
Xenbase
Kahn,
Determinants of substrate and cation affinities in the Na+/dicarboxylate cotransporter.
1999,
Pubmed
Kekuda,
Primary structure and functional characteristics of a mammalian sodium-coupled high affinity dicarboxylate transporter.
1999,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Ledvina,
Negative electrostatic surface potential of protein sites specific for anionic ligands.
1996,
Pubmed
Lostao,
Arginine-427 in the Na+/glucose cotransporter (SGLT1) is involved in trafficking to the plasma membrane.
1995,
Pubmed
,
Xenbase
Markovich,
Expression cloning of rat renal Na+/SO4(2-) cotransport.
1993,
Pubmed
,
Xenbase
Pajor,
Mutational analysis of histidine residues in the rabbit Na+/dicarboxylate co-transporter NaDC-1.
1998,
Pubmed
,
Xenbase
Pajor,
The substrate recognition domain in the Na+/dicarboxylate and Na+/sulfate cotransporters is located in the carboxy-terminal portion of the protein.
1998,
Pubmed
,
Xenbase
Pajor,
Sodium and lithium interactions with the Na+/Dicarboxylate cotransporter.
1998,
Pubmed
,
Xenbase
Pajor,
Sodium-coupled transporters for Krebs cycle intermediates.
1999,
Pubmed
,
Xenbase
Pajor,
Sequence and functional characterization of a renal sodium/dicarboxylate cotransporter.
1995,
Pubmed
,
Xenbase
Pajor,
Molecular cloning and functional expression of a sodium-dicarboxylate cotransporter from human kidney.
1996,
Pubmed
,
Xenbase
Pajor,
Functional differences between rabbit and human Na(+)-dicarboxylate cotransporters, NaDC-1 and hNaDC-1.
1996,
Pubmed
,
Xenbase
Parent,
Electrogenic properties of the cloned Na+/glucose cotransporter: II. A transport model under nonrapid equilibrium conditions.
1992,
Pubmed
Quiocho,
Atomic basis of the exquisite specificity of phosphate and sulfate transport receptors.
1996,
Pubmed
Rüdiger,
Specific arginine and threonine residues control anion binding and transport in the light-driven chloride pump halorhodopsin.
1997,
Pubmed
Sekine,
Cloning, functional characterization, and localization of a rat renal Na+-dicarboxylate transporter.
1998,
Pubmed
,
Xenbase
Steffgen,
Expression cloning and characterization of a novel sodium-dicarboxylate cotransporter from winter flounder kidney.
1999,
Pubmed
,
Xenbase
Teasdale,
Signal-mediated sorting of membrane proteins between the endoplasmic reticulum and the golgi apparatus.
1996,
Pubmed
Wright,
Kinetics of sodium succinate cotransport across renal brush-border membranes.
1983,
Pubmed
Zerangue,
A new ER trafficking signal regulates the subunit stoichiometry of plasma membrane K(ATP) channels.
1999,
Pubmed
,
Xenbase
