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Association of human immunodeficiency virus type 1 Vif with RNA and its role in reverse transcription.
Dettenhofer M
,
Cen S
,
Carlson BA
,
Kleiman L
,
Yu XF
.
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The vif gene of human immunodeficiency virus type 1 (HIV-1) is essential for viral replication, although the functional target of Vif remains elusive. HIV-1 vif mutant virions derived from nonpermissive H9 cells displayed no significant differences in the amount, ratio, or integrity of their protein composition relative to an isogenic wild-type virion. The amounts of the virion-associated viral genomic RNA and tRNA(3)(Lys) were additionally present at normal levels in vif mutant virions. We demonstrate that Vif associates with RNA in vitro as well as with viral genomic RNA in virus-infected cells. A functionally conserved lentivirus Vif motif was found in the double-stranded RNA binding domain of Xenopus laevis, Xlrbpa. The natural intravirion reverse transcriptase products were markedly reduced in vif mutant virions. Moreover, purified vif mutant genomic RNA-primer tRNA complexes displayed severe defects in the initiation of reverse transcription with recombinant reverse transcriptase. These data point to a novel role for Vif in the regulation of efficient reverse transcription through modulation of the virion nucleic acid components.
Bouyac,
Phenotypically Vif- human immunodeficiency virus type 1 is produced by chronically infected restrictive cells.
1997, Pubmed
Bouyac,
Phenotypically Vif- human immunodeficiency virus type 1 is produced by chronically infected restrictive cells.
1997,
Pubmed
Burd,
Conserved structures and diversity of functions of RNA-binding proteins.
1994,
Pubmed
Cen,
The role of Pr55(gag) in the annealing of tRNA3Lys to human immunodeficiency virus type 1 genomic RNA.
1999,
Pubmed
Chatterji,
Feline immunodeficiency virus Vif localizes to the nucleus.
2000,
Pubmed
Cobrinik,
A retroviral RNA secondary structure required for efficient initiation of reverse transcription.
1988,
Pubmed
Courcoul,
Peripheral blood mononuclear cells produce normal amounts of defective Vif- human immunodeficiency virus type 1 particles which are restricted for the preretrotranscription steps.
1995,
Pubmed
Dettenhofer,
Highly purified human immunodeficiency virus type 1 reveals a virtual absence of Vif in virions.
1999,
Pubmed
Dornadula,
Partial rescue of the Vif-negative phenotype of mutant human immunodeficiency virus type 1 strains from nonpermissive cells by intravirion reverse transcription.
2000,
Pubmed
Draper,
Protein-RNA recognition.
1995,
Pubmed
Eckmann,
Xlrbpa, a double-stranded RNA-binding protein associated with ribosomes and heterogeneous nuclear RNPs.
1997,
Pubmed
,
Xenbase
Fouchier,
Human immunodeficiency virus type 1 Vif does not influence expression or virion incorporation of gag-, pol-, and env-encoded proteins.
1996,
Pubmed
Goncalves,
Role of Vif in human immunodeficiency virus type 1 reverse transcription.
1996,
Pubmed
Goncalves,
Subcellular localization of the Vif protein of human immunodeficiency virus type 1.
1994,
Pubmed
Harmache,
Requirement of caprine arthritis encephalitis virus vif gene for in vivo replication.
1996,
Pubmed
Hatfield,
Chromatographic analysis of the aminoacyl-tRNAs which are required for translation of codons at and around the ribosomal frameshift sites of HIV, HTLV-1, and BLV.
1989,
Pubmed
Huang,
Effects of modifying the tRNA(3Lys) anticodon on the initiation of human immunodeficiency virus type 1 reverse transcription.
1996,
Pubmed
Huang,
Primer tRNA3Lys on the viral genome exists in unextended and two-base extended forms within mature human immunodeficiency virus type 1.
1997,
Pubmed
Huang,
The role of nucleocapsid and U5 stem/A-rich loop sequences in tRNA(3Lys) genomic placement and initiation of reverse transcription in human immunodeficiency virus type 1.
1998,
Pubmed
Inoshima,
Roles of the auxiliary genes and AP-1 binding site in the long terminal repeat of feline immunodeficiency virus in the early stage of infection in cats.
1996,
Pubmed
Isel,
Specific initiation and switch to elongation of human immunodeficiency virus type 1 reverse transcription require the post-transcriptional modifications of primer tRNA3Lys.
1996,
Pubmed
Isel,
Modified nucleotides of tRNA(3Lys) modulate primer/template loop-loop interaction in the initiation complex of HIV-1 reverse transcription.
1993,
Pubmed
Kan,
Identification of HTLV-III/LAV sor gene product and detection of antibodies in human sera.
1986,
Pubmed
Lee,
A new HTLV-III/LAV protein encoded by a gene found in cytopathic retroviruses.
1986,
Pubmed
Li,
Multiple forms of tRNA(Lys3) in HIV-1.
1996,
Pubmed
Lori,
Viral DNA carried by human immunodeficiency virus type 1 virions.
1992,
Pubmed
Madani,
An endogenous inhibitor of human immunodeficiency virus in human lymphocytes is overcome by the viral Vif protein.
1998,
Pubmed
Mak,
Primer tRNAs for reverse transcription.
1997,
Pubmed
Marquet,
tRNAs as primer of reverse transcriptases.
1995,
Pubmed
Miller,
Multiple biological roles associated with the Rous sarcoma virus 5' untranslated RNA U5-IR stem and loop.
1997,
Pubmed
Nascimbeni,
The replicative impairment of Vif- mutants of human immunodeficiency virus type 1 correlates with an overall defect in viral DNA synthesis.
1998,
Pubmed
Oberste,
Conservation of amino-acid sequence motifs in lentivirus Vif proteins.
1992,
Pubmed
Ochsenbauer,
Analysis of vif-defective human immunodeficiency virus type 1 (HIV-1) virions synthesized in 'non-permissive' T lymphoid cells stably infected with selectable HIV-1.
1997,
Pubmed
Park,
Effects of vif mutations on cell-free infectivity and replication of simian immunodeficiency virus.
1994,
Pubmed
Ryter,
Molecular basis of double-stranded RNA-protein interactions: structure of a dsRNA-binding domain complexed with dsRNA.
1998,
Pubmed
,
Xenbase
Simon,
The human immunodeficiency virus type 1 Vif protein modulates the postpenetration stability of viral nucleoprotein complexes.
1996,
Pubmed
Simon,
The Vif and Gag proteins of human immunodeficiency virus type 1 colocalize in infected human T cells.
1997,
Pubmed
Simon,
Evidence for a newly discovered cellular anti-HIV-1 phenotype.
1998,
Pubmed
Simon,
Vif and the p55(Gag) polyprotein of human immunodeficiency virus type 1 are present in colocalizing membrane-free cytoplasmic complexes.
1999,
Pubmed
Sodroski,
Replicative and cytopathic potential of HTLV-III/LAV with sor gene deletions.
1986,
Pubmed
Sova,
Efficiency of viral DNA synthesis during infection of permissive and nonpermissive cells with vif-negative human immunodeficiency virus type 1.
1993,
Pubmed
St Johnston,
A conserved double-stranded RNA-binding domain.
1992,
Pubmed
,
Xenbase
Tomonaga,
Identification of a feline immunodeficiency virus gene which is essential for cell-free virus infectivity.
1992,
Pubmed
Trono,
Partial reverse transcripts in virions from human immunodeficiency and murine leukemia viruses.
1992,
Pubmed
von Schwedler,
Vif is crucial for human immunodeficiency virus type 1 proviral DNA synthesis in infected cells.
1993,
Pubmed
