Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-15107
Science 1998 Apr 10;2805361:284-6.
Show Gene links Show Anatomy links

Conservation of substrate recognition mechanisms by tRNA splicing endonucleases.

Fabbri S , Fruscoloni P , Bufardeci E , Di Nicola Negri E , Baldi MI , Attardi DG , Mattoccia E , Tocchini-Valentini GP .


???displayArticle.abstract???
Accuracy in transfer RNA (tRNA) splicing is essential for the formation of functional tRNAs, and hence for gene expression, in both Eukaryotes and Archaea. The specificity for recognition of the tRNA precursor (pre-tRNA) resides in the endonuclease, which removes the intron by making two independent endonucleolytic cleavages. Although the eukaryal and archaeal enzymes appear to use different features of pre-tRNAs to determine the sites of cleavage, analysis of hybrid pre-tRNA substrates containing eukaryal and archaeal sequences, described here, reveals that the eukaryal enzyme retains the ability to use the archaeal recognition signals. This result indicates that there may be a common ancestral mechanism for recognition of pre-tRNA by proteins.

???displayArticle.pubmedLink??? 9535657
???displayArticle.link??? Science


Genes referenced: mt-tr trna