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Genesis
2007 Nov 01;4511:667-78. doi: 10.1002/dvg.20340.
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Small heat shock protein Hsp27 is required for proper heart tube formation.
Brown DD
,
Christine KS
,
Showell C
,
Conlon FL
.
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The small heat shock protein Hsp27 has been shown to be involved in a diverse array of cellular processes, including cellular stress response, protein chaperone activity, regulation of cellular glutathione levels, apoptotic signaling, and regulation of actin polymerization and stability. Furthermore, mutation within Hsp27 has been associated with the human congenital neuropathy Charcot-Marie Tooth (CMT) disease. Hsp27 is known to be expressed in developing embryonic tissues; however, little has been done to determine the endogenous requirement for Hsp27 in developing embryos. In this study, we show that depletion of XHSP27 protein results in a failure of cardiac progenitor fusion resulting in cardia bifida. Furthermore, we demonstrate a concomitant disorganization of actin filament organization and defects in myofibril assembly. Moreover, these defects are not associated with alterations in specification or differentiation. We have thus demonstrated a critical requirement for XHSP27 in developing cardiac and skeletal muscle tissues.
FIG. 2. XHsp27 is expressed in
the gastrula, and developing skeletal
and cardiac muscle. Whole
mount in situ hybridization of X.
laevis embryos using an antisense
probe specific for XHsp27 at the
indicated stages. (a) Dorsal is to
the top. (b�i) Anterior is to the left.
(j�m) Transverse sections through
somitic (j, k) and cardiac (l, m)
regions. Dorsal is to the top. b,
body muscle; br, brain; h, heart; j,
jaw muscle, m, myotome; mc,
myocardium.
Alexander,
casanova plays an early and essential role in endoderm formation in zebrafish.
1999, Pubmed,
Xenbase
Alexander,
casanova plays an early and essential role in endoderm formation in zebrafish.
1999,
Pubmed
,
Xenbase
Arrigo,
Hsp27: novel regulator of intracellular redox state.
2001,
Pubmed
Baek,
Role of small heat shock protein HSP25 in radioresistance and glutathione-redox cycle.
2000,
Pubmed
Beiman,
Heartless, a Drosophila FGF receptor homolog, is essential for cell migration and establishment of several mesodermal lineages.
1996,
Pubmed
Benndorf,
Shocking degeneration.
2004,
Pubmed
Benndorf,
Phosphorylation and supramolecular organization of murine small heat shock protein HSP25 abolish its actin polymerization-inhibiting activity.
1994,
Pubmed
Bonham,
Hsp27, Hsp70, and metallothionein in MDCK and LLC-PK1 renal epithelial cells: effects of prolonged exposure to cadmium.
2003,
Pubmed
Brown,
Developmental expression of the Xenopus laevis Tbx20 orthologue.
2003,
Pubmed
,
Xenbase
Brown,
Tbx5 and Tbx20 act synergistically to control vertebrate heart morphogenesis.
2005,
Pubmed
,
Xenbase
Brown,
Xtn3 is a developmentally expressed cardiac and skeletal muscle-specific novex-3 titin isoform.
2006,
Pubmed
,
Xenbase
Bruey,
Hsp27 negatively regulates cell death by interacting with cytochrome c.
2000,
Pubmed
Brundel,
Induction of heat shock response protects the heart against atrial fibrillation.
2006,
Pubmed
Ciruna,
Expression of the T-box gene Eomesodermin during early mouse development.
1999,
Pubmed
,
Xenbase
DEHAAN,
Migration patterns of the precardiac mesoderm in the early chick embrvo.
1963,
Pubmed
Dalle-Donne,
The actin cytoskeleton response to oxidants: from small heat shock protein phosphorylation to changes in the redox state of actin itself.
2001,
Pubmed
David,
Perinatal expression of heat-shock protein 27 in brain regions and nonneural tissues of the piglet.
2000,
Pubmed
Escobedo,
HSP25 protects skeletal muscle cells against oxidative stress.
2004,
Pubmed
Evgrafov,
Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.
2004,
Pubmed
Feil,
A novel quaternary structure of the dimeric alpha-crystallin domain with chaperone-like activity.
2001,
Pubmed
Ferns,
Heat shock protein 27: its potential role in vascular disease.
2006,
Pubmed
George,
Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin.
1993,
Pubmed
George,
Fibronectins are essential for heart and blood vessel morphogenesis but are dispensable for initial specification of precursor cells.
1997,
Pubmed
Gernold,
Development and tissue-specific distribution of mouse small heat shock protein hsp25.
1993,
Pubmed
Gisselbrecht,
heartless encodes a fibroblast growth factor receptor (DFR1/DFGF-R2) involved in the directional migration of early mesodermal cells in the Drosophila embryo.
1996,
Pubmed
Goetz,
TBX5 is required for embryonic cardiac cell cycle progression.
2006,
Pubmed
,
Xenbase
Goetz,
Cardiac progenitors and the embryonic cell cycle.
2007,
Pubmed
Harland,
In situ hybridization: an improved whole-mount method for Xenopus embryos.
1991,
Pubmed
,
Xenbase
Hollander,
Overexpression of wild-type heat shock protein 27 and a nonphosphorylatable heat shock protein 27 mutant protects against ischemia/reperfusion injury in a transgenic mouse model.
2004,
Pubmed
Huot,
HSP27 phosphorylation-mediated resistance against actin fragmentation and cell death induced by oxidative stress.
1996,
Pubmed
Jakob,
Small heat shock proteins are molecular chaperones.
1993,
Pubmed
Jiang,
The Xenopus GATA-4/5/6 genes are associated with cardiac specification and can regulate cardiac-specific transcription during embryogenesis.
1996,
Pubmed
,
Xenbase
Kikuchi,
The zebrafish bonnie and clyde gene encodes a Mix family homeodomain protein that regulates the generation of endodermal precursors.
2000,
Pubmed
,
Xenbase
Knowlton,
Differential expression of heat shock proteins in normal and failing human hearts.
1998,
Pubmed
Kolker,
Confocal imaging of early heart development in Xenopus laevis.
2000,
Pubmed
,
Xenbase
Komatsuda,
Overexpression of the human 72 kDa heat shock protein in renal tubular cells confers resistance against oxidative injury and cisplatin toxicity.
1999,
Pubmed
Kuo,
GATA4 transcription factor is required for ventral morphogenesis and heart tube formation.
1997,
Pubmed
Kupperman,
A sphingosine-1-phosphate receptor regulates cell migration during vertebrate heart development.
2000,
Pubmed
Langdon,
SHP-2 is required for the maintenance of cardiac progenitors.
2007,
Pubmed
,
Xenbase
Lavoie,
Induction of Chinese hamster HSP27 gene expression in mouse cells confers resistance to heat shock. HSP27 stabilization of the microfilament organization.
1993,
Pubmed
Lavoie,
Modulation of actin microfilament dynamics and fluid phase pinocytosis by phosphorylation of heat shock protein 27.
1993,
Pubmed
Lavoie,
Modulation of cellular thermoresistance and actin filament stability accompanies phosphorylation-induced changes in the oligomeric structure of heat shock protein 27.
1995,
Pubmed
Leal,
Lead-stimulated p38MAPK-dependent Hsp27 phosphorylation.
2002,
Pubmed
Liu,
Over-expression of heat shock protein 27 attenuates doxorubicin-induced cardiac dysfunction in mice.
2007,
Pubmed
Lough,
Endoderm and heart development.
2000,
Pubmed
Mao,
Cloning, characterization, and heat stress-induced redistribution of a protein homologous to human hsp27 in the zebrafish Danio rerio.
2005,
Pubmed
Mao,
Developmentally regulated gene expression of the small heat shock protein Hsp27 in zebrafish embryos.
2006,
Pubmed
Mehlen,
Intracellular reactive oxygen species as apparent modulators of heat-shock protein 27 (hsp27) structural organization and phosphorylation in basal and tumour necrosis factor alpha-treated T47D human carcinoma cells.
1995,
Pubmed
Mehlen,
hsp27 as a switch between differentiation and apoptosis in murine embryonic stem cells.
1997,
Pubmed
Miron,
A 25-kD inhibitor of actin polymerization is a low molecular mass heat shock protein.
1991,
Pubmed
Mohun,
The origins of cardiac tissue in the amphibian, Xenopus laevis.
2003,
Pubmed
,
Xenbase
Paul,
Hsp27 as a negative regulator of cytochrome C release.
2002,
Pubmed
Quertermous,
Apelin and its g protein-coupled receptor regulate cardiac development as well as cardiac function.
2007,
Pubmed
Rahman,
The Saccharomyces cerevisiae small heat shock protein Hsp26 inhibits actin polymerisation.
1995,
Pubmed
Reiter,
Gata5 is required for the development of the heart and endoderm in zebrafish.
1999,
Pubmed
,
Xenbase
Reynolds,
A review of heat shock protein induction following cerebellar injury.
2003,
Pubmed
Scheler,
Comparison of two-dimensional electrophoresis patterns of heat shock protein Hsp27 species in normal and cardiomyopathic hearts.
1999,
Pubmed
Schier,
The one-eyed pinhead gene functions in mesoderm and endoderm formation in zebrafish and interacts with no tail.
1997,
Pubmed
Scott,
The g protein-coupled receptor agtrl1b regulates early development of myocardial progenitors.
2007,
Pubmed
,
Xenbase
Shama,
Transient up-regulation of myotonic dystrophy protein kinase-binding protein, MKBP, and HSP27 in the neonatal myocardium.
1999,
Pubmed
Shelden,
Heat shock protein 27 associates with basolateral cell boundaries in heat-shocked and ATP-depleted epithelial cells.
2002,
Pubmed
Skre,
Genetic and clinical aspects of Charcot-Marie-Tooth's disease.
1974,
Pubmed
Somji,
Heat shock protein 27 expression in human proximal tubule cells exposed to lethal and sublethal concentrations of CdCl2.
1999,
Pubmed
Stainier,
Zebrafish genetics and vertebrate heart formation.
2001,
Pubmed
Sun,
Targeted disruption of Fgf8 causes failure of cell migration in the gastrulating mouse embryo.
1999,
Pubmed
Trinh,
Fibronectin regulates epithelial organization during myocardial migration in zebrafish.
2004,
Pubmed
Tuttle,
Analysis of the expression and function of the small heat shock protein gene, hsp27, in Xenopus laevis embryos.
2007,
Pubmed
,
Xenbase
Vander Heide,
Increased expression of HSP27 protects canine myocytes from simulated ischemia-reperfusion injury.
2002,
Pubmed
Wilson,
Induction of epidermis and inhibition of neural fate by Bmp-4.
1995,
Pubmed
,
Xenbase
Yelon,
The bHLH transcription factor hand2 plays parallel roles in zebrafish heart and pectoral fin development.
2000,
Pubmed
Zeng,
Apelin and its receptor control heart field formation during zebrafish gastrulation.
2007,
Pubmed
,
Xenbase
