Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The origin of the rRNA precursor from Xenopus borealis, analysed in vivo and in vitro.
McStay B
,
Bird A
.
???displayArticle.abstract???
We have determined the origin of the major transcript of Xenopus borealis rDNA by the use of an SI nuclease protection assay. The DNA surrounding the origin of this transcript was sequenced, and the region upstream of the origin was shown to have strong sequence homology with that region from X.laevis rDNA. We have also demonstrated faithful transcription from this origin using cloned X.borealis rDNA in an extract derived from X. laevis culture cells. This in vitro transcription was insensitive to 100 micrograms/ml alpha-amanatin, suggesting that it was mediated by RNA polymerase 1.
Bach,
Sequence organization of the spacer in the ribosomal genes of Xenopus clivii and Xenopus borealis.
1981, Pubmed,
Xenbase
Bach,
Sequence organization of the spacer in the ribosomal genes of Xenopus clivii and Xenopus borealis.
1981,
Pubmed
,
Xenbase
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Boseley,
Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Brown,
A comparison of the ribosomal DNA's of Xenopus laevis and Xenopus mulleri: the evolution of tandem genes.
1972,
Pubmed
,
Xenbase
Coen,
Multiple Pol I initiation sequences in rDNA spacers of Drosophila melanogaster.
1982,
Pubmed
,
Xenbase
Forsheit,
An electron microscope heteroduplex study of the ribosomal DNAs of Xenopus laevis and Xenopus mulleri.
1974,
Pubmed
,
Xenbase
Garoff,
Improvements of DNA sequencing gels.
1981,
Pubmed
Grummt,
Specific transcription of mouse ribosomal DNA in a cell-free system that mimics control in vivo.
1981,
Pubmed
Grummt,
Ribosomal RNA transcription in vitro is species specific.
1982,
Pubmed
Honjo,
Preferential transcription of Xenopus laevis ribosomal RNA in interspecies hybrids between Xenopus laevis and Xenopus mulleri.
1973,
Pubmed
,
Xenbase
Kohorn,
Accurate transcription of truncated ribosomal DNA templates in a Drosophila cell-free system.
1982,
Pubmed
Kohorn,
Nontranscribed spacer sequences promote in vitro transcription of Drosophila ribosomal DNA.
1982,
Pubmed
La Volpe,
DNaseI-hypersensitive sites at promoter-like sequences in the spacer of Xenopus laevis and Xenopus borealis ribosomal DNA.
1983,
Pubmed
,
Xenbase
Macleod,
DNAase I sensitivity and methylation of active versus inactive rRNA genes in xenopus species hybrids.
1982,
Pubmed
,
Xenbase
Marshall,
Synthesis of ribosomal RNA in synkaryons and heterokaryons formed between human and rodent cells.
1975,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Miesfeld,
Identification of the in vivo and in vitro origin of transcription in human rDNA.
1982,
Pubmed
Miller,
Kinetics of accumulation and processing of simian virus 40 RNA in Xenopus laevis oocytes injected with simian virus 40 DNA.
1982,
Pubmed
,
Xenbase
Miller,
Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells.
1976,
Pubmed
Miller,
Expression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids.
1976,
Pubmed
Miller,
Transcription of mouse rRNA genes by RNA polymerase I: in vitro and in vivo initiation and processing sites.
1981,
Pubmed
Miller,
Transcription of the 'non-transcribed' spacer of Drosophila melanogaster rDNA.
1983,
Pubmed
Moss,
The putative promoter of a Xenopus laevis ribosomal gene is reduplicated.
1979,
Pubmed
,
Xenbase
Moss,
More ribosomal spacer sequences from Xenopus laevis.
1980,
Pubmed
,
Xenbase
Moss,
Transcription of cloned Xenopus laevis ribosomal DNA microinjected into Xenopus oocytes, and the identification of an RNA polymerase I promoter.
1982,
Pubmed
,
Xenbase
Moss,
A transcriptional function for the repetitive ribosomal spacer in Xenopus laevis.
,
Pubmed
,
Xenbase
Perry,
Selective suppression of the transcription of ribosomal genes in mouse-human hybrid cells.
1979,
Pubmed
Reeder,
Evidence for two functional regions in the Xenopus laevis RNA polymerase I promoter.
1983,
Pubmed
,
Xenbase
Rüther,
pUR222, a vector for cloning and rapid chemical sequencing of DNA.
1981,
Pubmed
Simeone,
Sequence organization of the ribosomal spacer of D.melanogaster.
1982,
Pubmed
Sollner-Webb,
The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
1979,
Pubmed
,
Xenbase
Sollner-Webb,
Accurate transcription of cloned Xenopus rRNA genes by RNA polymerase I: demonstration by S1 nuclease mapping.
1982,
Pubmed
,
Xenbase
Vieira,
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
1982,
Pubmed
