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Dev Biol 2012 Mar 01;3631:155-65. doi: 10.1016/j.ydbio.2011.12.029.
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RFX2 is broadly required for ciliogenesis during vertebrate development.

Chung MI , Peyrot SM , LeBoeuf S , Park TJ , McGary KL , Marcotte EM , Wallingford JB .

In Caenorhabditis elegans, the RFX (Daf19) transcription factor is a major regulator of ciliogenesis, controlling the expression of the many essential genes required for making cilia. In vertebrates, however, seven RFX genes have been identified. Bioinformatic analysis suggests that Rfx2 is among the closest homologues of Daf19. We therefore hypothesize that Rfx2 broadly controls ciliogenesis during vertebrate development. Indeed, here we show that Rfx2 in Xenopus is expressed preferentially in ciliated tissues, including neural tube, gastrocoel roof plate, epidermal multi-ciliated cells, otic vesicles, and kidneys. Knockdown of Rfx2 results in cilia-defective embryonic phenotypes and fewer or truncated cilia are observed in Rfx2 morphants. These results indicate that Rfx2 is broadly required for ciliogenesis in vertebrates. Furthermore, we show that Rfx2 is essential for expression of several ciliogenic genes, including TTC25, which we show here is required for ciliogenesis, HH signaling, and left-right patterning.

PubMed ID: 22227339
PMC ID: PMC3640985
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: arl13b cdh1 foxj1 lefty nkx2-2 odad4 pitx2 rfx1 rfx2 rfx3 rfx4 rfx5 shh tuba8 tubal3 vax1
Antibodies: Arl13b Ab1 Tuba4b Ab3
Morpholinos: odad4 MO1 rfx2 MO1

Article Images: [+] show captions
References [+] :
Aftab, Identification and characterization of novel human tissue-specific RFX transcription factors. 2008, Pubmed