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Dev Dyn 2006 Oct 01;23510:2811-6. doi: 10.1002/dvdy.20909.
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Enhanced sensitivity and stability in two-color in situ hybridization by means of a novel chromagenic substrate combination.

Hurtado R , Mikawa T .

Double in situ hybridization analysis is a fundamental technique for studying the expression of two genes with high temporal and spatial resolution. However, due to the lack of sensitivity in current detection methods, this approach is powerful only when at least one transcript is abundantly expressed. Here, we report a new enzyme/chromagenic substrate combination that provides sufficient sensitivity for detecting two less abundant transcripts and stability for subsequent paraffin sectioning.

PubMed ID: 16894600
Article link: Dev Dyn

Species referenced: Xenopus
Genes referenced: fgf8 pax2 shh tbx5 tubb2b

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