Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Differentiation 2008 Apr 01;764:392-403. doi: 10.1111/j.1432-0436.2007.00229.x.
Show Gene links Show Anatomy links

The efficiency of Xenopus primordial germ cell migration depends on the germplasm mRNA encoding the PDZ domain protein Grip2.

Kirilenko P , Weierud FK , Zorn AM , Woodland HR .

A microarray analysis of vegetal pole sequences in the egg and early Xenopus laevis embryo identified Unigene Xl.14891 as a vegetally localized RNA. Analysis of the Xenopus tropicalis genome showed this Unigene to be localized near the 3' end of the Grip2 (glutamate receptor interacting protein 2) transcription unit. RACE showed that the Unigene represented the 3' UTR of Grip2 mRNA. Grip2 mRNA is present in the mitochondrial cloud of late pre-vitellogenic oocytes and then in the germplasm through oogenesis and early development until tailbud tadpole stages. Interference with Grip2 mRNA translation using two antisense morpholino oligos (MOs) impairs primordial germ cell (PGC) migration to the germinal ridges. Both MOs also inhibit swimming movements of the tailbud tadpole, known to involve glutamate receptors. We conclude that Grip2 has several functions in the embryo, including enabling efficient PGC migration.

PubMed ID: 17924960
Article link: Differentiation
Grant support: [+]

Species referenced: Xenopus tropicalis Xenopus laevis
Genes referenced: cyp26a1 grip1 grip2 myc pgat pgc
Antibodies: Myc Ab3 Tubg1 Ab2
Morpholinos: grip2 MO3 grip2 MO4

Article Images: [+] show captions